Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904408094> ?p ?o ?g. }
- W2904408094 abstract "Abstract Multicellular organisms interact with resident microbes in important ways, and a better understanding of host-microbe interactions is aided by tools such as high-throughput 16S sequencing. However, rigorous evaluation of the veracity of these tools in a different context from which they were developed has often lagged behind. Our goal was to perform one such critical test by examining how variation in tissue preparation and DNA isolation could affect inferences about gut microbiome variation between two genetically divergent lines of threespine stickleback fish maintained in the same lab environment. Using careful experimental design and intensive sampling of individuals, we addressed technical and biological sources of variation in 16S-based estimates of microbial diversity. After employing a two-tiered bead beating approach consisting of tissue homogenization followed by microbial lysis in subsamples, we found an extremely minor effect of DNA isolation protocol relative to among-host microbial diversity differences. Individual abundance estimates for rare OTUs, however, showed much lower reproducibility. We found that the stickleback gut microbiome was highly variable, even among siblings housed together, but that an effect of host genotype (stickleback lineage) was detectable for some microbial taxa. Our findings demonstrate the importance of appropriately quantifying biological and technical variance components when attempting to understand major influences on high-throughput microbiome data." @default.
- W2904408094 created "2018-12-22" @default.
- W2904408094 creator A5014073911 @default.
- W2904408094 creator A5031237279 @default.
- W2904408094 creator A5037820992 @default.
- W2904408094 creator A5078920198 @default.
- W2904408094 creator A5091798934 @default.
- W2904408094 date "2018-12-17" @default.
- W2904408094 modified "2023-10-18" @default.
- W2904408094 title "Highly reproducible 16S sequencing facilitates measurement of host genetic influences on the stickleback gut microbiome" @default.
- W2904408094 cites W1951724000 @default.
- W2904408094 cites W1968423560 @default.
- W2904408094 cites W1970777915 @default.
- W2904408094 cites W1988925586 @default.
- W2904408094 cites W1989981486 @default.
- W2904408094 cites W2012133321 @default.
- W2904408094 cites W2012139809 @default.
- W2904408094 cites W2013103271 @default.
- W2904408094 cites W2015303573 @default.
- W2904408094 cites W2017917281 @default.
- W2904408094 cites W2023902482 @default.
- W2904408094 cites W2029781810 @default.
- W2904408094 cites W2038802458 @default.
- W2904408094 cites W2048619570 @default.
- W2904408094 cites W2055615060 @default.
- W2904408094 cites W2071841602 @default.
- W2904408094 cites W2072970694 @default.
- W2904408094 cites W2080283023 @default.
- W2904408094 cites W2090110425 @default.
- W2904408094 cites W2092696502 @default.
- W2904408094 cites W2098762803 @default.
- W2904408094 cites W2104318549 @default.
- W2904408094 cites W2108477241 @default.
- W2904408094 cites W2111609787 @default.
- W2904408094 cites W2124351063 @default.
- W2904408094 cites W2128236594 @default.
- W2904408094 cites W2128769815 @default.
- W2904408094 cites W2145104573 @default.
- W2904408094 cites W2145664556 @default.
- W2904408094 cites W2148422863 @default.
- W2904408094 cites W2153402526 @default.
- W2904408094 cites W2160292265 @default.
- W2904408094 cites W2161377204 @default.
- W2904408094 cites W2162737864 @default.
- W2904408094 cites W2166655850 @default.
- W2904408094 cites W2170024099 @default.
- W2904408094 cites W2170245265 @default.
- W2904408094 cites W2170486072 @default.
- W2904408094 cites W2178518272 @default.
- W2904408094 cites W2267013337 @default.
- W2904408094 cites W2299616256 @default.
- W2904408094 cites W2316149929 @default.
- W2904408094 cites W2343323301 @default.
- W2904408094 cites W2401404581 @default.
- W2904408094 cites W2490800126 @default.
- W2904408094 cites W2520725961 @default.
- W2904408094 cites W2559088919 @default.
- W2904408094 cites W2587230861 @default.
- W2904408094 cites W2593905635 @default.
- W2904408094 cites W2728822570 @default.
- W2904408094 cites W2731562184 @default.
- W2904408094 cites W2757289041 @default.
- W2904408094 cites W2761370067 @default.
- W2904408094 cites W2762425175 @default.
- W2904408094 cites W2766981973 @default.
- W2904408094 cites W2776695687 @default.
- W2904408094 cites W2783764624 @default.
- W2904408094 cites W2787902098 @default.
- W2904408094 cites W2803457956 @default.
- W2904408094 cites W2805009792 @default.
- W2904408094 cites W2806007080 @default.
- W2904408094 cites W4242702784 @default.
- W2904408094 doi "https://doi.org/10.1101/497792" @default.
- W2904408094 hasPublicationYear "2018" @default.
- W2904408094 type Work @default.
- W2904408094 sameAs 2904408094 @default.
- W2904408094 citedByCount "1" @default.
- W2904408094 countsByYear W29044080942019 @default.
- W2904408094 crossrefType "posted-content" @default.
- W2904408094 hasAuthorship W2904408094A5014073911 @default.
- W2904408094 hasAuthorship W2904408094A5031237279 @default.
- W2904408094 hasAuthorship W2904408094A5037820992 @default.
- W2904408094 hasAuthorship W2904408094A5078920198 @default.
- W2904408094 hasAuthorship W2904408094A5091798934 @default.
- W2904408094 hasBestOaLocation W29044080941 @default.
- W2904408094 hasConcept C105795698 @default.
- W2904408094 hasConcept C126831891 @default.
- W2904408094 hasConcept C130217890 @default.
- W2904408094 hasConcept C143121216 @default.
- W2904408094 hasConcept C151730666 @default.
- W2904408094 hasConcept C18903297 @default.
- W2904408094 hasConcept C2778938320 @default.
- W2904408094 hasConcept C2779343474 @default.
- W2904408094 hasConcept C2779969263 @default.
- W2904408094 hasConcept C2780558290 @default.
- W2904408094 hasConcept C2781162219 @default.
- W2904408094 hasConcept C2909208804 @default.
- W2904408094 hasConcept C33923547 @default.
- W2904408094 hasConcept C505870484 @default.
- W2904408094 hasConcept C51679486 @default.