Matches in SemOpenAlex for { <https://semopenalex.org/work/W290440813> ?p ?o ?g. }
- W290440813 endingPage "112" @default.
- W290440813 startingPage "89" @default.
- W290440813 abstract "Transglutaminase (TGase) is able to catalyse the acyl transfer reaction between the γ-carboxamide group of a protein-bound glutamine (Gln) residue and an amino-derivative of poly(ethylene glycol) (PEG-NH2), thus leading to a PEGylated protein. Several proteins of therapeutic interest have been PEGylated by means of TGase, among them interleukin-2, granulocyte colony-stimulating factor, human growth hormone and erythropoietin. Surprisingly, PEGylation occurred at specific Gln residue(s), despite the fact that these proteins contained several Gln residues. An analysis of the TGase-mediated reactions in terms of structure and dynamics of protein substrates revealed a correlation between sites of TGase attack and chain regions of enhanced backbone flexibility, as detected by the crystallographic profile of the B-factor along the protein polypeptide chain. Moreover, the TGasemediated reactions often occurred at chain regions characterized by missing electron density, indicating that these regions are disordered. In particular, it was noted that in a number of cases the sites of TGase attack occurred at the same chain regions prone to limited proteolysis phenomena. Since chain flexibility or local unfolding was earlier found to dictate the sites of limited proteolysis of proteins, it is concluded that both TGase and a protease require an unfolded polypeptide substrate in an extended conformation for the site-specific enzymatic attack." @default.
- W290440813 created "2016-06-24" @default.
- W290440813 creator A5015439157 @default.
- W290440813 creator A5020606836 @default.
- W290440813 creator A5022652502 @default.
- W290440813 creator A5080110814 @default.
- W290440813 date "2009-01-01" @default.
- W290440813 modified "2023-09-27" @default.
- W290440813 title "The site-specific TGase-mediated PEGylation of proteins occurs at flexible sites" @default.
- W290440813 cites W1067939983 @default.
- W290440813 cites W127277662 @default.
- W290440813 cites W137241693 @default.
- W290440813 cites W1494258879 @default.
- W290440813 cites W1500201678 @default.
- W290440813 cites W1502809559 @default.
- W290440813 cites W1507155150 @default.
- W290440813 cites W1560546800 @default.
- W290440813 cites W1572633452 @default.
- W290440813 cites W1578857975 @default.
- W290440813 cites W1709580310 @default.
- W290440813 cites W1964944514 @default.
- W290440813 cites W1965413803 @default.
- W290440813 cites W1965880798 @default.
- W290440813 cites W1970266799 @default.
- W290440813 cites W1970898345 @default.
- W290440813 cites W1971086299 @default.
- W290440813 cites W1973429118 @default.
- W290440813 cites W1974877787 @default.
- W290440813 cites W1979721017 @default.
- W290440813 cites W1980694228 @default.
- W290440813 cites W1982597750 @default.
- W290440813 cites W1982978980 @default.
- W290440813 cites W1983411104 @default.
- W290440813 cites W1984349476 @default.
- W290440813 cites W1984965754 @default.
- W290440813 cites W1985469117 @default.
- W290440813 cites W1987754109 @default.
- W290440813 cites W1989067119 @default.
- W290440813 cites W1997814933 @default.
- W290440813 cites W1999616324 @default.
- W290440813 cites W1999778444 @default.
- W290440813 cites W2005416074 @default.
- W290440813 cites W2005458331 @default.
- W290440813 cites W2007829086 @default.
- W290440813 cites W2010528256 @default.
- W290440813 cites W2013270869 @default.
- W290440813 cites W2014576677 @default.
- W290440813 cites W2017482945 @default.
- W290440813 cites W2019575728 @default.
- W290440813 cites W202042235 @default.
- W290440813 cites W2021646539 @default.
- W290440813 cites W2022234371 @default.
- W290440813 cites W2023147041 @default.
- W290440813 cites W2023670848 @default.
- W290440813 cites W2024847531 @default.
- W290440813 cites W2024854170 @default.
- W290440813 cites W2025864476 @default.
- W290440813 cites W2027896020 @default.
- W290440813 cites W2030661378 @default.
- W290440813 cites W2030855909 @default.
- W290440813 cites W2031061484 @default.
- W290440813 cites W2031218469 @default.
- W290440813 cites W2034590798 @default.
- W290440813 cites W2035690849 @default.
- W290440813 cites W2038727363 @default.
- W290440813 cites W2039680648 @default.
- W290440813 cites W2044199954 @default.
- W290440813 cites W2047066026 @default.
- W290440813 cites W2048283280 @default.
- W290440813 cites W2049355382 @default.
- W290440813 cites W2049470926 @default.
- W290440813 cites W2051788484 @default.
- W290440813 cites W2051879599 @default.
- W290440813 cites W2052011238 @default.
- W290440813 cites W2052606462 @default.
- W290440813 cites W2054634611 @default.
- W290440813 cites W2055805017 @default.
- W290440813 cites W2056903291 @default.
- W290440813 cites W2056975293 @default.
- W290440813 cites W2057641915 @default.
- W290440813 cites W2057971165 @default.
- W290440813 cites W2058930880 @default.
- W290440813 cites W2059432632 @default.
- W290440813 cites W2059977761 @default.
- W290440813 cites W2062482701 @default.
- W290440813 cites W2062915105 @default.
- W290440813 cites W2063067093 @default.
- W290440813 cites W2063425971 @default.
- W290440813 cites W2065120981 @default.
- W290440813 cites W2067403820 @default.
- W290440813 cites W2068274053 @default.
- W290440813 cites W2069280006 @default.
- W290440813 cites W2072969500 @default.
- W290440813 cites W2073443024 @default.
- W290440813 cites W2073745520 @default.
- W290440813 cites W2074113996 @default.
- W290440813 cites W2078160959 @default.
- W290440813 cites W2078414857 @default.