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- W2904452573 abstract "The recent development of tools to decipher the intricacies of neural networks has improved our understanding of brain function. Optogenetics allows one to assess the direct outcome of activating a genetically-distinct population of neurons. Neurons are tagged with light-sensitive channels followed by photo-activation with an appropriate wavelength of light to functionally activate or silence them, resulting in quantifiable changes in the periphery. Capturing and manipulating activated neuron ensembles, is a recently-designed technique to permanently label activated neurons responsible for a physiological function and manipulate them. On the other hand, neurons can be transfected with genetically-encoded Ca2+ indicators to capture the interplay between them that modulates autonomic end-points or somatic behavior. These techniques work with millisecond temporal precision. In addition, neurons can be manipulated chronically to simulate physiological aberrations by transfecting designer G-protein-coupled receptors exclusively activated by designer drugs. In this review, we elaborate on the fundamental concepts and applications of these techniques in research." @default.
- W2904452573 created "2018-12-22" @default.
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- W2904452573 creator A5050387959 @default.
- W2904452573 date "2018-12-17" @default.
- W2904452573 modified "2023-09-25" @default.
- W2904452573 title "Next-Generation Tools to Study Autonomic Regulation In Vivo" @default.
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- W2904452573 doi "https://doi.org/10.1007/s12264-018-0319-2" @default.
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