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• W2904608049 abstract "Single-cell analysis is a rapidly evolving to characterize molecular information at the individual cell level. Here, we present a new approach with the potential to overcome several key challenges facing the currently available techniques. The approach is based on the identification of volatile organic compounds (VOCs), viz. organic compounds having relatively high vapor pressure, emitted to the cell's headspace. This concept is demonstrated using lung cancer cells with various p53 genetic status and normal lung cells. The VOCs were analyzed by gas chromatography combined with mass spectrometry. Among hundreds of detected compounds, 18 VOCs showed significant changes in their concentration levels in tumor cells versus control. The composition of these VOCs was found to depend, also, on the sub-molecular structure of the p53 genetic status. Analyzing the VOCs offers a complementary way of querying the molecular mechanisms of cancer as well as of developing new generation(s) of biomedical approaches for personalized screening and diagnosis." @default.
• W2904608049 created "2018-12-22" @default.
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• W2904608049 date "2019-01-01" @default.
• W2904608049 modified "2023-10-11" @default.
• W2904608049 title "Profiling Single Cancer Cells with Volatolomics Approach" @default.
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• W2904608049 doi "https://doi.org/10.1016/j.isci.2018.12.008" @default.
• W2904608049 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6319329" @default.
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