FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904670295> ?p ?o ?g. }

• W2904670295 endingPage "e0208976" @default.
• W2904670295 startingPage "e0208976" @default.
• W2904670295 abstract "Carbapenemase-producing Enterobacteriaceae (CPE) are a global concern because these bacteria are resistant to almost all β-lactams. Horizontal interspecies gene transfer via plasmid conjugation has increased the global dissemination of CPE. Recently, an Enterobacteriaceae strain positive for carbapenemase gene but showing a carbapenem-susceptible phenotype was identified, suggesting that these susceptible strains may be challenging to detect solely via antimicrobial susceptibility tests without molecular analysis. Here, we isolated a blaIMP-6 carbapenemase-gene positive but imipenem- and meropenem-susceptible Escherichia coli (ISMS-E) strain A56-1S (imipenem and meropenem minimum inhibitory concentration, ≤ 0.125 mg/L), from a human urine specimen in Japan. A56-1S was carbapenemase negative by the Carba NP test, suggesting that IMP-6 production was low or undetectable. Thus, to characterize the mechanism of this phenotype, a meropenem-resistant E. coli A56-1R strain was obtained using meropenem-selection. A56-1R was positive for carbapenemase production by the Carba NP test, and blaIMP-6 transcription in A56-1R was 53-fold higher than in A56-1S, indicating that blaIMP-6 in A56-1S is negatively regulated at the transcriptional level. Comparative genomic analysis between the two strains revealed that the alleviation of restriction of DNA (ardK) gene encoding a putative transcription factor is disrupted by the IS26 insertion in A56-1R. A cotransformation assay of ardK and the regulatory element upstream of blaIMP-6 showed repression of blaIMP-6 transcription, indicating that ArdK negatively modulates blaIMP-6 transcription. ArdK binding and affinity assays demonstrated that ArdK directly binds to the regulatory element upstream of blaIMP-6 with dissociation constant values comparable to those of general transcription factors. The IMP-6 carbapenemase showed low hydrolytic activity against imipenem, resulting in an imipenem-susceptible and meropenem-resistant (ISMR) phenotype (previously reported as a stealth phenotype). However, the low expression of IMP-6 in the A56-1S strain could be a typical characteristic of ISMS-E due to gene repression, indicating that conventional antimicrobial susceptibility tests might be unable to detect such strains even when using both imipenem and meropenem. Bacteria that exhibit the ISMS phenotype could play a potential role as undetectable reservoirs and might facilitate gene transfer to other organisms while avoiding detection." @default.
• W2904670295 created "2018-12-22" @default.
• W2904670295 creator A5002785073 @default.
• W2904670295 creator A5026878477 @default.
• W2904670295 creator A5031470659 @default.
• W2904670295 creator A5053108028 @default.
• W2904670295 creator A5062642490 @default.
• W2904670295 creator A5079291451 @default.
• W2904670295 date "2018-12-11" @default.
• W2904670295 modified "2023-10-16" @default.
• W2904670295 title "The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene blaIMP-6, leading to a carbapenem-susceptible phenotype in the blaIMP-6-positive Escherichia coli strain A56-1S" @default.
• W2904670295 cites W1524357749 @default.
• W2904670295 cites W1584524384 @default.
• W2904670295 cites W1763400538 @default.
• W2904670295 cites W1798266128 @default.
• W2904670295 cites W1860032350 @default.
• W2904670295 cites W1983403167 @default.
• W2904670295 cites W2007748295 @default.
• W2904670295 cites W2019891171 @default.
• W2904670295 cites W2060057324 @default.
• W2904670295 cites W2065337857 @default.
• W2904670295 cites W2065380874 @default.
• W2904670295 cites W2070302799 @default.
• W2904670295 cites W2102722490 @default.
• W2904670295 cites W2105161608 @default.
• W2904670295 cites W2107572188 @default.
• W2904670295 cites W2114307066 @default.
• W2904670295 cites W2118701853 @default.
• W2904670295 cites W2139580695 @default.
• W2904670295 cites W2145037511 @default.
• W2904670295 cites W2145041871 @default.
• W2904670295 cites W2146502343 @default.
• W2904670295 cites W2146535783 @default.
• W2904670295 cites W2205496271 @default.
• W2904670295 cites W2294356061 @default.
• W2904670295 cites W2332271404 @default.
• W2904670295 cites W2565160152 @default.
• W2904670295 doi "https://doi.org/10.1371/journal.pone.0208976" @default.
• W2904670295 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6289460" @default.
• W2904670295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30533034" @default.
• W2904670295 hasPublicationYear "2018" @default.
• W2904670295 type Work @default.
• W2904670295 sameAs 2904670295 @default.
• W2904670295 citedByCount "8" @default.
• W2904670295 countsByYear W29046702952020 @default.
• W2904670295 countsByYear W29046702952021 @default.
• W2904670295 countsByYear W29046702952022 @default.
• W2904670295 countsByYear W29046702952023 @default.
• W2904670295 crossrefType "journal-article" @default.
• W2904670295 hasAuthorship W2904670295A5002785073 @default.
• W2904670295 hasAuthorship W2904670295A5026878477 @default.
• W2904670295 hasAuthorship W2904670295A5031470659 @default.
• W2904670295 hasAuthorship W2904670295A5053108028 @default.
• W2904670295 hasAuthorship W2904670295A5062642490 @default.
• W2904670295 hasAuthorship W2904670295A5079291451 @default.
• W2904670295 hasBestOaLocation W29046702951 @default.
• W2904670295 hasConcept C104317684 @default.
• W2904670295 hasConcept C127716648 @default.
• W2904670295 hasConcept C22744801 @default.
• W2904670295 hasConcept C2776685102 @default.
• W2904670295 hasConcept C2778910516 @default.
• W2904670295 hasConcept C2779375183 @default.
• W2904670295 hasConcept C2779631663 @default.
• W2904670295 hasConcept C501593827 @default.
• W2904670295 hasConcept C523546767 @default.
• W2904670295 hasConcept C54355233 @default.
• W2904670295 hasConcept C547475151 @default.
• W2904670295 hasConcept C86339819 @default.
• W2904670295 hasConcept C86803240 @default.
• W2904670295 hasConcept C89423630 @default.
• W2904670295 hasConcept C94665300 @default.
• W2904670295 hasConceptScore W2904670295C104317684 @default.
• W2904670295 hasConceptScore W2904670295C127716648 @default.
• W2904670295 hasConceptScore W2904670295C22744801 @default.
• W2904670295 hasConceptScore W2904670295C2776685102 @default.
• W2904670295 hasConceptScore W2904670295C2778910516 @default.
• W2904670295 hasConceptScore W2904670295C2779375183 @default.
• W2904670295 hasConceptScore W2904670295C2779631663 @default.
• W2904670295 hasConceptScore W2904670295C501593827 @default.
• W2904670295 hasConceptScore W2904670295C523546767 @default.
• W2904670295 hasConceptScore W2904670295C54355233 @default.
• W2904670295 hasConceptScore W2904670295C547475151 @default.
• W2904670295 hasConceptScore W2904670295C86339819 @default.
• W2904670295 hasConceptScore W2904670295C86803240 @default.
• W2904670295 hasConceptScore W2904670295C89423630 @default.
• W2904670295 hasConceptScore W2904670295C94665300 @default.
• W2904670295 hasFunder F4320311405 @default.
• W2904670295 hasIssue "12" @default.
• W2904670295 hasLocation W29046702951 @default.
• W2904670295 hasLocation W29046702952 @default.
• W2904670295 hasLocation W29046702953 @default.
• W2904670295 hasLocation W29046702954 @default.
• W2904670295 hasLocation W29046702955 @default.
• W2904670295 hasOpenAccess W2904670295 @default.
• W2904670295 hasPrimaryLocation W29046702951 @default.
• W2904670295 hasRelatedWork W1970948691 @default.
• W2904670295 hasRelatedWork W2006661458 @default.
• W2904670295 hasRelatedWork W2040402235 @default.

• next