Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904672337> ?p ?o ?g. }
- W2904672337 endingPage "928" @default.
- W2904672337 startingPage "911" @default.
- W2904672337 abstract "Many nascent long non-coding RNAs (lncRNAs) undergo the same maturation steps as pre-mRNAs of protein-coding genes (PCGs), but they are often poorly spliced. To identify the underlying mechanisms for this phenomenon, we searched for putative splicing inhibitory sequences using the ncRNA-a2 as a model. Genome-wide analyses of intergenic lncRNAs (lincRNAs) revealed that lincRNA splicing efficiency positively correlates with 5'ss strength while no such correlation was identified for PCGs. In addition, efficiently spliced lincRNAs have higher thymidine content in the polypyrimidine tract (PPT) compared to efficiently spliced PCGs. Using model lincRNAs, we provide experimental evidence that strengthening the 5'ss and increasing the T content in PPT significantly enhances lincRNA splicing. We further showed that lincRNA exons contain less putative binding sites for SR proteins. To map binding of SR proteins to lincRNAs, we performed iCLIP with SRSF2, SRSF5 and SRSF6 and analyzed eCLIP data for SRSF1, SRSF7 and SRSF9. All examined SR proteins bind lincRNA exons to a much lower extent than expression-matched PCGs. We propose that lincRNAs lack the cooperative interaction network that enhances splicing, which renders their splicing outcome more dependent on the optimality of splice sites." @default.
- W2904672337 created "2018-12-22" @default.
- W2904672337 creator A5000159179 @default.
- W2904672337 creator A5000512157 @default.
- W2904672337 creator A5007915098 @default.
- W2904672337 creator A5012957405 @default.
- W2904672337 creator A5023141985 @default.
- W2904672337 creator A5049780559 @default.
- W2904672337 creator A5055213139 @default.
- W2904672337 date "2018-11-16" @default.
- W2904672337 modified "2023-10-15" @default.
- W2904672337 title "Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5′ splice-site sequences due to weak interactions with SR proteins" @default.
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