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- W2904691429 abstract "The HIPPO signaling pathway plays an early and essential role in mammalian embryogenesis. The earliest known roles for HIPPO signaling during mouse development include segregating fetal and extraembryonic lineages and establishing the pluripotent progenitors of embryonic stem (ES) cells. In the mouse early embryo, HIPPO signaling responds to multiple cell biological inputs, including cell polarization, cytoskeleton, and cell environment, to influence gene expression and the first cell fate decisions in development. Methods to monitor and manipulate HIPPO signaling in the mouse early embryo are fundamental to discovering mechanisms regulating pluripotency in vivo, but properties of the early embryo, such as small cell number and spherical architecture, pose unique challenges for signaling pathway analysis. Here, we share approaches for visualizing HIPPO signaling in mouse early embryos. In addition, these methods can be applied to visualize HIPPO signaling in other spherical or cystic structures comprised of relatively few cells, such as organoids, or for the examination of other signaling pathways in these contexts." @default.
- W2904691429 created "2018-12-22" @default.
- W2904691429 creator A5010531486 @default.
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- W2904691429 date "2018-12-19" @default.
- W2904691429 modified "2023-09-29" @default.
- W2904691429 title "Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development" @default.
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- W2904691429 doi "https://doi.org/10.1007/978-1-4939-8910-2_25" @default.
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