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- W2904702013 abstract "The effects of various metabolic inhibitors on genetic recombination of bacteriophage T4 were studied. Treatment of mixedly-infected cells with 8 × 10−3 M -KCN increased the frequency of recombination between two rII mutants in progeny formed after release from inhibition. During a prolonged treatment with these inhibitors the infected cells produced progeny particles which included recombinants in high proportions. A similar effect was observed by treatment with 8 × 10−3 M -As205, 2 × 10−3 M -NaN3 or 10−2 M -2,4-dinitrophenol. Keeping the infected cells in buffer also increased the frequency of recombinants in progeny subsequently formed after addition of nutrients. During treatment of infected cells with KCN very little DNA synthesis took place. After treatment of cells infected with 32P-labelled phage particles with KCN, infective centres did not change in sensitivity to decay of incorporated 32P. It is therefore concluded that phage chromosomes tend to recombine rather than to grow during the treatment. These inhibitors could be used to separate recombination from replication of phage chromosomes" @default.
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- W2904702013 date "1964-04-01" @default.
- W2904702013 modified "2023-09-27" @default.
- W2904702013 title "Molecular mechanisms of genetic recombination in bacteriophage" @default.
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- W2904702013 doi "https://doi.org/10.1016/s0022-2836(64)80008-5" @default.
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