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- W2904703576 abstract "Objective: The aim of this study was to develop and validate analytical methods for determining methamphetamine in saliva using gas chromatographytandemmass spectrometry (MS).Methods: The chromatography conditions were DB MS-5 capillary columns with a length of 30 m, inner diameter of 0.25 mm, mobile phase of Heliumgas 99.999%, flow rate of 0.8 mL/min, detection of MS at m/z values of 58.00 and 91.00, respectively, and ephedrine HCl as the internal standard.Results: The validation of analytical methods for methamphetamine satisfies the validation criteria by the EMEA Guidelines 2011. Bioanalyticalmethods obtained were linear in the concentration range from 15.0 to 300.0 ng/mL with r>0.9999. Sample preparation was done using liquid–liquidmicroextraction with cyclohexane, supernatant residue was dried and reconstituted with approximately 100 μL of methanol.Conclusion: The method was successfully applied to saliva samples of methamphetamine users with levels in the range of test." @default.
- W2904703576 created "2018-12-22" @default.
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- W2904703576 date "2018-12-20" @default.
- W2904703576 modified "2023-09-24" @default.
- W2904703576 title "DEVELOPMENT AND VALIDATION OF METHAMPHETAMINE ANALYSIS IN SALIVA USING GAS CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY" @default.
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- W2904703576 doi "https://doi.org/10.22159/ijap.2018.v10s1.20" @default.
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