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- W290476640 abstract "We have developed an improved Agrobacterium-mediated protocol in plum (Prunus domestica L.) through the addition of 2,4-D to the regeneration media. This method has increased the regeneration efficiency of independent transgenic plants up to 10 fold over previous reports. DNA blot analysis of putative transgenic shoots revealed transformation efficiencies of up to 42% with an average of 25% over all trials. The method as described produces self-rooted transgenic plants in approximately 6 months. The high transformation rates coupled with the rapid plant establishment methodology makes it possible to utilize plum transformation both for the introduction of agronomically useful genes into this species, and as a model plant for functional genomics studies in Prunus spp., rosaceaous species, and woody plants in general." @default.
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- W290476640 date "2009-07-01" @default.
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- W290476640 title "A HIGH-THROUGHPUT TRANSFORMATION SYSTEM IN PLUM (PRUNUS DOMESTICA L.) USEFUL FOR FUNCTIONAL GENOMICS IN ROSACEAE" @default.
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- W290476640 doi "https://doi.org/10.17660/actahortic.2009.839.48" @default.
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