Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904805680> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2904805680 endingPage "388" @default.
- W2904805680 startingPage "381" @default.
- W2904805680 abstract "Abstract Breast cancer is the most frequent cancer among women worldwide. Ki67 can be used as an immunohistochemical pseudo marker for cell proliferation to determine how aggressive the cancer is and thereby the treatment of the patient. No standard Ki67 staining protocol exists, resulting in inter‐laboratory stain variability. Therefore, it is important to determine the quality control of a staining protocol to ensure correct diagnosis and treatment of patients. Currently, quality control is performed by the organization NordiQC that use an expert panel‐based qualitative assessment system. However, no objective method exists to determine the quality of a staining protocol. In this study, we propose an algorithm, to objectively assess staining quality from segmented cell nuclei structures extracted from cell lines. The cell nuclei were classified into either Ki67 positive or negative to determine the Ki67 proliferation index within the cell lines. A Ki67 stain quality model based on ordinal logistic regression was developed to determine the quality of a staining protocol from features extracted from the segmented cell nuclei in the cell lines. The algorithm was able to segment and classify Ki67 positive cell nuclei with a sensitivity and positive predictive value (PPV) of 0.90 and 0.94 and Ki67 negative cell nuclei with a sensitivity and PPV of 0.78 and 0.78. The mean difference between a manual and automatic Ki67 proliferation index was −0.003 with a standard deviation of 0.056. The ordinal logistic regression model found that the stain intensity for both the Ki67 positive and Ki67 negative cell nuclei were statistically significant as parameters determining the stain quality from the cell line cores. The framework shows great promise for using cell nuclei information from cell lines to predict the staining quality of staining protocols. © 2018 International Society for Advancement of Cytometry" @default.
- W2904805680 created "2018-12-22" @default.
- W2904805680 creator A5029886041 @default.
- W2904805680 creator A5051498477 @default.
- W2904805680 creator A5056959729 @default.
- W2904805680 creator A5060015430 @default.
- W2904805680 creator A5061115495 @default.
- W2904805680 creator A5081958083 @default.
- W2904805680 creator A5090327258 @default.
- W2904805680 date "2018-12-17" @default.
- W2904805680 modified "2023-10-16" @default.
- W2904805680 title "Quality assessment of Ki67 staining using cell line proliferation index and stain intensity features" @default.
- W2904805680 cites W1757407923 @default.
- W2904805680 cites W1956163040 @default.
- W2904805680 cites W2004369799 @default.
- W2904805680 cites W2016038678 @default.
- W2904805680 cites W2088603417 @default.
- W2904805680 cites W2104586902 @default.
- W2904805680 cites W2109722393 @default.
- W2904805680 cites W2123269393 @default.
- W2904805680 cites W2133059825 @default.
- W2904805680 cites W2137935883 @default.
- W2904805680 cites W2145023731 @default.
- W2904805680 cites W2416490606 @default.
- W2904805680 doi "https://doi.org/10.1002/cyto.a.23683" @default.
- W2904805680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30556331" @default.
- W2904805680 hasPublicationYear "2018" @default.
- W2904805680 type Work @default.
- W2904805680 sameAs 2904805680 @default.
- W2904805680 citedByCount "5" @default.
- W2904805680 countsByYear W29048056802020 @default.
- W2904805680 countsByYear W29048056802021 @default.
- W2904805680 countsByYear W29048056802022 @default.
- W2904805680 crossrefType "journal-article" @default.
- W2904805680 hasAuthorship W2904805680A5029886041 @default.
- W2904805680 hasAuthorship W2904805680A5051498477 @default.
- W2904805680 hasAuthorship W2904805680A5056959729 @default.
- W2904805680 hasAuthorship W2904805680A5060015430 @default.
- W2904805680 hasAuthorship W2904805680A5061115495 @default.
- W2904805680 hasAuthorship W2904805680A5081958083 @default.
- W2904805680 hasAuthorship W2904805680A5090327258 @default.
- W2904805680 hasBestOaLocation W29048056801 @default.
- W2904805680 hasConcept C126322002 @default.
- W2904805680 hasConcept C142724271 @default.
- W2904805680 hasConcept C151956035 @default.
- W2904805680 hasConcept C204232928 @default.
- W2904805680 hasConcept C2781294515 @default.
- W2904805680 hasConcept C2910938049 @default.
- W2904805680 hasConcept C54355233 @default.
- W2904805680 hasConcept C62112901 @default.
- W2904805680 hasConcept C71924100 @default.
- W2904805680 hasConcept C74864618 @default.
- W2904805680 hasConcept C86803240 @default.
- W2904805680 hasConceptScore W2904805680C126322002 @default.
- W2904805680 hasConceptScore W2904805680C142724271 @default.
- W2904805680 hasConceptScore W2904805680C151956035 @default.
- W2904805680 hasConceptScore W2904805680C204232928 @default.
- W2904805680 hasConceptScore W2904805680C2781294515 @default.
- W2904805680 hasConceptScore W2904805680C2910938049 @default.
- W2904805680 hasConceptScore W2904805680C54355233 @default.
- W2904805680 hasConceptScore W2904805680C62112901 @default.
- W2904805680 hasConceptScore W2904805680C71924100 @default.
- W2904805680 hasConceptScore W2904805680C74864618 @default.
- W2904805680 hasConceptScore W2904805680C86803240 @default.
- W2904805680 hasIssue "4" @default.
- W2904805680 hasLocation W29048056801 @default.
- W2904805680 hasLocation W29048056802 @default.
- W2904805680 hasOpenAccess W2904805680 @default.
- W2904805680 hasPrimaryLocation W29048056801 @default.
- W2904805680 hasRelatedWork W183829867 @default.
- W2904805680 hasRelatedWork W1992481203 @default.
- W2904805680 hasRelatedWork W2010086444 @default.
- W2904805680 hasRelatedWork W2013745628 @default.
- W2904805680 hasRelatedWork W2035464821 @default.
- W2904805680 hasRelatedWork W2070285969 @default.
- W2904805680 hasRelatedWork W2333235522 @default.
- W2904805680 hasRelatedWork W2365674884 @default.
- W2904805680 hasRelatedWork W3097795298 @default.
- W2904805680 hasRelatedWork W3214902097 @default.
- W2904805680 hasVolume "95" @default.
- W2904805680 isParatext "false" @default.
- W2904805680 isRetracted "false" @default.
- W2904805680 magId "2904805680" @default.
- W2904805680 workType "article" @default.