FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2904808051> ?p ?o ?g. }

• W2904808051 endingPage "3925" @default.
• W2904808051 startingPage "3925" @default.
• W2904808051 abstract "The CRISPR/Cas9 system has been widely used for targeted genome editing in numerous plant species. In Arabidopsis, constitutive promoters usually result in a low efficiency of heritable mutation in the T1 generation. In this work, CRISPR/Cas9 gene editing efficiencies using different promoters to drive Cas9 expression were evaluated. Expression of Cas9 under the constitutive CaMV 35S promoter resulted in a 2.3% mutation rate in T1 plants and failed to produce homozygous mutations in the T1 and T2 generations. In contrast, expression of Cas9 under two cell division-specific promoters, YAO and CDC45, produced mutation rates of 80.9% to 100% in the T1 generation with nonchimeric mutations in the T1 (4.4–10%) and T2 (32.5–46.1%) generations. The pCDC45 promoter was used to modify a previously reported multiplex CRISPR/Cas9 system, replacing the original constitutive ubiquitin promoter. The multi-pCDC45-Cas9 system produced higher mutation efficiencies than the multi-pUBQ-Cas9 system in the T1 generation (60.17% vs. 43.71%) as well as higher efficiency of heritable mutations (11.30% vs. 4.31%). Sextuple T2 homozygous mutants were identified from a construct targeting seven individual loci. Our results demonstrate the advantage of using cell division promoters for CRISPR/Cas9 gene editing applications in Arabidopsis, especially in multiplex applications." @default.
• W2904808051 created "2018-12-22" @default.
• W2904808051 creator A5005204434 @default.
• W2904808051 creator A5020563221 @default.
• W2904808051 creator A5049293054 @default.
• W2904808051 creator A5051930466 @default.
• W2904808051 creator A5066652500 @default.
• W2904808051 creator A5068002544 @default.
• W2904808051 creator A5086571248 @default.
• W2904808051 date "2018-12-07" @default.
• W2904808051 modified "2023-09-28" @default.
• W2904808051 title "A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in Arabidopsis" @default.
• W2904808051 cites W1869792624 @default.
• W2904808051 cites W1913575892 @default.
• W2904808051 cites W1937054661 @default.
• W2904808051 cites W1982802227 @default.
• W2904808051 cites W1982846895 @default.
• W2904808051 cites W1990379499 @default.
• W2904808051 cites W2001845502 @default.
• W2904808051 cites W2003467310 @default.
• W2904808051 cites W2024199022 @default.
• W2904808051 cites W2037623448 @default.
• W2904808051 cites W2043496004 @default.
• W2904808051 cites W2053427298 @default.
• W2904808051 cites W2064815984 @default.
• W2904808051 cites W2073412341 @default.
• W2904808051 cites W2081261052 @default.
• W2904808051 cites W2107494694 @default.
• W2904808051 cites W2112444085 @default.
• W2904808051 cites W2129086389 @default.
• W2904808051 cites W2136656609 @default.
• W2904808051 cites W2139342899 @default.
• W2904808051 cites W2140886363 @default.
• W2904808051 cites W2153344788 @default.
• W2904808051 cites W2165016943 @default.
• W2904808051 cites W2166671294 @default.
• W2904808051 cites W2168740468 @default.
• W2904808051 cites W2169244146 @default.
• W2904808051 cites W2170326957 @default.
• W2904808051 cites W2191974075 @default.
• W2904808051 cites W2196121581 @default.
• W2904808051 cites W2200172833 @default.
• W2904808051 cites W2210852389 @default.
• W2904808051 cites W2398269215 @default.
• W2904808051 cites W2416811494 @default.
• W2904808051 cites W2808821825 @default.
• W2904808051 doi "https://doi.org/10.3390/ijms19123925" @default.
• W2904808051 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6321140" @default.
• W2904808051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30544514" @default.
• W2904808051 hasPublicationYear "2018" @default.
• W2904808051 type Work @default.
• W2904808051 sameAs 2904808051 @default.
• W2904808051 citedByCount "38" @default.
• W2904808051 countsByYear W29048080512019 @default.
• W2904808051 countsByYear W29048080512020 @default.
• W2904808051 countsByYear W29048080512021 @default.
• W2904808051 countsByYear W29048080512022 @default.
• W2904808051 countsByYear W29048080512023 @default.
• W2904808051 crossrefType "journal-article" @default.
• W2904808051 hasAuthorship W2904808051A5005204434 @default.
• W2904808051 hasAuthorship W2904808051A5020563221 @default.
• W2904808051 hasAuthorship W2904808051A5049293054 @default.
• W2904808051 hasAuthorship W2904808051A5051930466 @default.
• W2904808051 hasAuthorship W2904808051A5066652500 @default.
• W2904808051 hasAuthorship W2904808051A5068002544 @default.
• W2904808051 hasAuthorship W2904808051A5086571248 @default.
• W2904808051 hasBestOaLocation W29048080511 @default.
• W2904808051 hasConcept C101762097 @default.
• W2904808051 hasConcept C104317684 @default.
• W2904808051 hasConcept C132455925 @default.
• W2904808051 hasConcept C143065580 @default.
• W2904808051 hasConcept C144501496 @default.
• W2904808051 hasConcept C150194340 @default.
• W2904808051 hasConcept C154055011 @default.
• W2904808051 hasConcept C2779491563 @default.
• W2904808051 hasConcept C501734568 @default.
• W2904808051 hasConcept C54355233 @default.
• W2904808051 hasConcept C70721500 @default.
• W2904808051 hasConcept C86803240 @default.
• W2904808051 hasConcept C98108389 @default.
• W2904808051 hasConceptScore W2904808051C101762097 @default.
• W2904808051 hasConceptScore W2904808051C104317684 @default.
• W2904808051 hasConceptScore W2904808051C132455925 @default.
• W2904808051 hasConceptScore W2904808051C143065580 @default.
• W2904808051 hasConceptScore W2904808051C144501496 @default.
• W2904808051 hasConceptScore W2904808051C150194340 @default.
• W2904808051 hasConceptScore W2904808051C154055011 @default.
• W2904808051 hasConceptScore W2904808051C2779491563 @default.
• W2904808051 hasConceptScore W2904808051C501734568 @default.
• W2904808051 hasConceptScore W2904808051C54355233 @default.
• W2904808051 hasConceptScore W2904808051C70721500 @default.
• W2904808051 hasConceptScore W2904808051C86803240 @default.
• W2904808051 hasConceptScore W2904808051C98108389 @default.
• W2904808051 hasIssue "12" @default.
• W2904808051 hasLocation W29048080511 @default.
• W2904808051 hasLocation W29048080512 @default.
• W2904808051 hasLocation W29048080513 @default.
• W2904808051 hasLocation W29048080514 @default.

• next