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• W2905013037 abstract "Mycobacterium manages to evade the host cell immune system, partially owing to its ability to survive redox stress after macrophage engulfment. Exposure to redox stress has been linked to later replication, persistence, and latent infection. In this work, mass spectrometry was used to elucidate the cell-wide changes that occur in response to sublethal doses of hydrogen peroxide and nitric oxide over time, with Mycobacterium smegmatis being used as a model organism. A total of 3135 proteins were confidently assigned, of which 1713, 1674, and 1713 were identified under NO, H2O2, and control conditions, respectively. Both treatment conditions resulted in changes of protein expression from the DosR regulon as well as those related to lipid metabolism. Complementary to the changes in the proteome, sublethal exposure to NO and H2O2 improved the survival of the bacteria after macrophage infection. Our data indicate that pre-exposure to sublethal doses of these redox stressors causes an alteration in the expression of proteins related to lipid metabolism, suggesting a link between altered lipid metabolism and enhanced survival in macrophages." @default.
• W2905013037 created "2018-12-22" @default.
• W2905013037 creator A5000499467 @default.
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• W2905013037 date "2018-12-01" @default.
• W2905013037 modified "2023-09-27" @default.
• W2905013037 title "Associating H₂O_2-and NO-related changes in the proteome of <i>Mycobacterium smegmatis</i> with enhanced survival in macrophage" @default.
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• W2905013037 doi "https://doi.org/10.1038/s41426-018-0210-2" @default.
• W2905013037 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6292918" @default.
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• W2905013037 hasPublicationYear "2018" @default.
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