FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2905089771> ?p ?o ?g. }

• W2905089771 endingPage "131" @default.
• W2905089771 startingPage "127" @default.
• W2905089771 abstract "The cellulolytic ability of fungal species is important to both natural and engineered biocycling of plant matter. One essential step is the conversion of cellobiose into glucose catalyzed by beta-glucosidases. Mutagenesis studies have implicated altering the substrate binding pocket to influence the pH-activity profile of this enzyme. However, structural understanding of the pH-affected substrate binding environment is lacking. Here we conducted molecular dynamics simulations of fully hydrated TrBgl2, a beta-glucosidase of Trichoderma reesei, equilibrated at its optimal pH (pH 6) and two unfavorable pHs (pH 5 and pH 7.5). We identified structural arrangement of specific residues that facilitated substrate escape from the catalytic site at pH 5 but locked the bound substrate in an unfavorable orientation at pH 7.5. For comparative analysis, we also performed simulations of a mutated TrBgl2 with previously demonstrated improved catalysis as a function of pH. We captured the responsible conformational changes in the engineered substrate binding pocket." @default.
• W2905089771 created "2018-12-22" @default.
• W2905089771 creator A5015600559 @default.
• W2905089771 creator A5030401347 @default.
• W2905089771 creator A5058083825 @default.
• W2905089771 date "2019-01-01" @default.
• W2905089771 modified "2023-09-26" @default.
• W2905089771 title "Substrate binding versus escape dynamics in a pH-affected fungal beta-glucosidase revealed by molecular dynamics simulations" @default.
• W2905089771 cites W1487346929 @default.
• W2905089771 cites W1922618753 @default.
• W2905089771 cites W1966078827 @default.
• W2905089771 cites W1969630046 @default.
• W2905089771 cites W1976499671 @default.
• W2905089771 cites W1987827372 @default.
• W2905089771 cites W2000597610 @default.
• W2905089771 cites W2000609901 @default.
• W2905089771 cites W2015642465 @default.
• W2905089771 cites W2018504228 @default.
• W2905089771 cites W2027687782 @default.
• W2905089771 cites W2029667189 @default.
• W2905089771 cites W2029890632 @default.
• W2905089771 cites W2035967016 @default.
• W2905089771 cites W2054447421 @default.
• W2905089771 cites W2057664120 @default.
• W2905089771 cites W2058001460 @default.
• W2905089771 cites W2064523054 @default.
• W2905089771 cites W2087377554 @default.
• W2905089771 cites W2103131593 @default.
• W2905089771 cites W2136038574 @default.
• W2905089771 cites W2148362226 @default.
• W2905089771 cites W2162166182 @default.
• W2905089771 cites W2169257298 @default.
• W2905089771 cites W2327345284 @default.
• W2905089771 cites W2740348174 @default.
• W2905089771 cites W967936445 @default.
• W2905089771 doi "https://doi.org/10.1016/j.carres.2018.12.007" @default.
• W2905089771 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30579119" @default.
• W2905089771 hasPublicationYear "2019" @default.
• W2905089771 type Work @default.
• W2905089771 sameAs 2905089771 @default.
• W2905089771 citedByCount "3" @default.
• W2905089771 countsByYear W29050897712020 @default.
• W2905089771 countsByYear W29050897712022 @default.
• W2905089771 countsByYear W29050897712023 @default.
• W2905089771 crossrefType "journal-article" @default.
• W2905089771 hasAuthorship W2905089771A5015600559 @default.
• W2905089771 hasAuthorship W2905089771A5030401347 @default.
• W2905089771 hasAuthorship W2905089771A5058083825 @default.
• W2905089771 hasBestOaLocation W29050897711 @default.
• W2905089771 hasConcept C104317684 @default.
• W2905089771 hasConcept C111368507 @default.
• W2905089771 hasConcept C12554922 @default.
• W2905089771 hasConcept C127313418 @default.
• W2905089771 hasConcept C141603559 @default.
• W2905089771 hasConcept C147597530 @default.
• W2905089771 hasConcept C161790260 @default.
• W2905089771 hasConcept C16318435 @default.
• W2905089771 hasConcept C181199279 @default.
• W2905089771 hasConcept C185592680 @default.
• W2905089771 hasConcept C2776125036 @default.
• W2905089771 hasConcept C2776519085 @default.
• W2905089771 hasConcept C2777289219 @default.
• W2905089771 hasConcept C2780120296 @default.
• W2905089771 hasConcept C2780412358 @default.
• W2905089771 hasConcept C41183919 @default.
• W2905089771 hasConcept C501734568 @default.
• W2905089771 hasConcept C55493867 @default.
• W2905089771 hasConcept C59593255 @default.
• W2905089771 hasConcept C71240020 @default.
• W2905089771 hasConcept C86803240 @default.
• W2905089771 hasConceptScore W2905089771C104317684 @default.
• W2905089771 hasConceptScore W2905089771C111368507 @default.
• W2905089771 hasConceptScore W2905089771C12554922 @default.
• W2905089771 hasConceptScore W2905089771C127313418 @default.
• W2905089771 hasConceptScore W2905089771C141603559 @default.
• W2905089771 hasConceptScore W2905089771C147597530 @default.
• W2905089771 hasConceptScore W2905089771C161790260 @default.
• W2905089771 hasConceptScore W2905089771C16318435 @default.
• W2905089771 hasConceptScore W2905089771C181199279 @default.
• W2905089771 hasConceptScore W2905089771C185592680 @default.
• W2905089771 hasConceptScore W2905089771C2776125036 @default.
• W2905089771 hasConceptScore W2905089771C2776519085 @default.
• W2905089771 hasConceptScore W2905089771C2777289219 @default.
• W2905089771 hasConceptScore W2905089771C2780120296 @default.
• W2905089771 hasConceptScore W2905089771C2780412358 @default.
• W2905089771 hasConceptScore W2905089771C41183919 @default.
• W2905089771 hasConceptScore W2905089771C501734568 @default.
• W2905089771 hasConceptScore W2905089771C55493867 @default.
• W2905089771 hasConceptScore W2905089771C59593255 @default.
• W2905089771 hasConceptScore W2905089771C71240020 @default.
• W2905089771 hasConceptScore W2905089771C86803240 @default.
• W2905089771 hasFunder F4320306076 @default.
• W2905089771 hasLocation W29050897711 @default.
• W2905089771 hasLocation W29050897712 @default.
• W2905089771 hasOpenAccess W2905089771 @default.
• W2905089771 hasPrimaryLocation W29050897711 @default.
• W2905089771 hasRelatedWork W1982946286 @default.
• W2905089771 hasRelatedWork W2014249415 @default.

• next