Matches in SemOpenAlex for { <https://semopenalex.org/work/W2905268953> ?p ?o ?g. }
- W2905268953 abstract "Deciphering biodistribution, biokinetics, and biological effects of nanoparticles (NPs) in entire organs with cellular resolution remains largely elusive due to the lack of effective imaging tools. Here, light sheet fluorescence microscopy in combination with optical tissue clearing was validated for concomitant three-dimensional mapping of lung morphology and NP biodistribution with cellular resolution in nondissected ex vivo murine lungs. Tissue autofluorescence allowed for label-free, quantitative morphometry of the entire bronchial tree, acinar structure, and blood vessels. Co-registration of fluorescent NPs with lung morphology revealed significant differences in pulmonary NP distribution depending on the means of application (intratracheal instillation and ventilator-assisted aerosol inhalation under anesthetized conditions). Inhalation exhibited a more homogeneous NP distribution in conducting airways and acini indicated by a central-to-peripheral (C/P) NP deposition ratio of unity (0.98 ± 0.13) as compared to a 2-fold enhanced central deposition (C/P = 1.98 ± 0.37) for instillation. After inhalation most NPs were observed in the proximal part of the acini as predicted by computational fluid dynamics simulations. At cellular resolution patchy NP deposition was visualized in bronchioles and acini, but more pronounced for instillation. Excellent linearity of the fluorescence intensity-dose response curve allowed for accurate NP dosimetry and revealed ca. 5% of the inhaled aerosol was deposited in the lungs. This single-modality imaging technique allows for quantitative co-registration of tissue architecture and NP biodistribution, which could accelerate elucidation of NP biokinetics and bioactivity within intact tissues, facilitating both nanotoxicology studies and the development of nanomedicines." @default.
- W2905268953 created "2018-12-22" @default.
- W2905268953 creator A5000400639 @default.
- W2905268953 creator A5037438061 @default.
- W2905268953 creator A5048287410 @default.
- W2905268953 creator A5052599627 @default.
- W2905268953 creator A5063307330 @default.
- W2905268953 creator A5066741040 @default.
- W2905268953 creator A5074211261 @default.
- W2905268953 creator A5077573086 @default.
- W2905268953 creator A5080424591 @default.
- W2905268953 creator A5082319905 @default.
- W2905268953 creator A5086970985 @default.
- W2905268953 creator A5089168114 @default.
- W2905268953 creator A5090579172 @default.
- W2905268953 date "2019-01-14" @default.
- W2905268953 modified "2023-09-29" @default.
- W2905268953 title "Three-Dimensional Quantitative Co-Mapping of Pulmonary Morphology and Nanoparticle Distribution with Cellular Resolution in Nondissected Murine Lungs" @default.
- W2905268953 cites W1963492502 @default.
- W2905268953 cites W1964157750 @default.
- W2905268953 cites W1969303395 @default.
- W2905268953 cites W1970884399 @default.
- W2905268953 cites W1977479596 @default.
- W2905268953 cites W1978811535 @default.
- W2905268953 cites W1994122881 @default.
- W2905268953 cites W1998222014 @default.
- W2905268953 cites W1999426707 @default.
- W2905268953 cites W2002261382 @default.
- W2905268953 cites W2008076337 @default.
- W2905268953 cites W2010956007 @default.
- W2905268953 cites W2031025318 @default.
- W2905268953 cites W2036934478 @default.
- W2905268953 cites W2040888382 @default.
- W2905268953 cites W2054874479 @default.
- W2905268953 cites W2068391384 @default.
- W2905268953 cites W2068628796 @default.
- W2905268953 cites W2069811863 @default.
- W2905268953 cites W2085714653 @default.
- W2905268953 cites W2093489232 @default.
- W2905268953 cites W2110194547 @default.
- W2905268953 cites W2110960563 @default.
- W2905268953 cites W2118088315 @default.
- W2905268953 cites W2120351270 @default.
- W2905268953 cites W2124397944 @default.
- W2905268953 cites W2127654256 @default.
- W2905268953 cites W2130166533 @default.
- W2905268953 cites W2138129903 @default.
- W2905268953 cites W2141970008 @default.
- W2905268953 cites W2146205672 @default.
- W2905268953 cites W2152440594 @default.
- W2905268953 cites W2157961970 @default.
- W2905268953 cites W2160027607 @default.
- W2905268953 cites W2165434098 @default.
- W2905268953 cites W2169862673 @default.
- W2905268953 cites W2189462190 @default.
- W2905268953 cites W2203529929 @default.
- W2905268953 cites W2257467030 @default.
- W2905268953 cites W2258599548 @default.
- W2905268953 cites W2300085948 @default.
- W2905268953 cites W2301340583 @default.
- W2905268953 cites W2302405003 @default.
- W2905268953 cites W2337208828 @default.
- W2905268953 cites W2343635104 @default.
- W2905268953 cites W2345941806 @default.
- W2905268953 cites W2425645630 @default.
- W2905268953 cites W2499712993 @default.
- W2905268953 cites W2529004273 @default.
- W2905268953 cites W2535387116 @default.
- W2905268953 cites W2547301282 @default.
- W2905268953 cites W2579972680 @default.
- W2905268953 cites W2587532232 @default.
- W2905268953 cites W2600897427 @default.
- W2905268953 cites W2602684234 @default.
- W2905268953 cites W2602815865 @default.
- W2905268953 cites W2609505052 @default.
- W2905268953 cites W2609730754 @default.
- W2905268953 cites W2623996050 @default.
- W2905268953 cites W2684817662 @default.
- W2905268953 cites W2684946613 @default.
- W2905268953 cites W2758338727 @default.
- W2905268953 cites W2775802685 @default.
- W2905268953 cites W2803998337 @default.
- W2905268953 cites W2885248577 @default.
- W2905268953 doi "https://doi.org/10.1021/acsnano.8b07524" @default.
- W2905268953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30566327" @default.
- W2905268953 hasPublicationYear "2019" @default.
- W2905268953 type Work @default.
- W2905268953 sameAs 2905268953 @default.
- W2905268953 citedByCount "24" @default.
- W2905268953 countsByYear W29052689532019 @default.
- W2905268953 countsByYear W29052689532020 @default.
- W2905268953 countsByYear W29052689532021 @default.
- W2905268953 countsByYear W29052689532022 @default.
- W2905268953 countsByYear W29052689532023 @default.
- W2905268953 crossrefType "journal-article" @default.
- W2905268953 hasAuthorship W2905268953A5000400639 @default.
- W2905268953 hasAuthorship W2905268953A5037438061 @default.
- W2905268953 hasAuthorship W2905268953A5048287410 @default.
- W2905268953 hasAuthorship W2905268953A5052599627 @default.
- W2905268953 hasAuthorship W2905268953A5063307330 @default.