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- W2905308045 abstract "The objectives of this project were to isolate Streptomyces from tropical soil samples inSabah; to screen for antimicrobial activities of Streptomyces against Escherichia coli0125, Vibrio parahaemolyticus and Salmonella typhi; to characterize the Streptomycespossessing antimicrobial activities and lastly to identify the Streptomyces by 16S rDNAsequencing. The materials and methods involved the sampling of tropical soil samples ofSST and FOR samples and were incubated at 31⁰C for four days after the addition ofCaCO₃ at 1: 1 ratio. The soil were diluted with sterile distilled water and plated ontoStreptomycetes agar (SA) containing cycloheximide at 30⁰C for several days. TheStreptomyces were then spot-inoculated onto a fresh SA with grid and incubated at 30°Cuntil sporulation. The plate was then being overlayed with soft agar containing targetpathogens as stated above and incubated overnight. After the formation of inhibitionzones, the Streptomyces was purified by restreaking the colony on fresh SA and wereGram-stained, KOH rapid test, and characterized. Then it was grown in broth and laterstored in 20% glycerol stock at -80°C. Its temperature, pH, and NaCl growth range werealso tested. The genotypic characterization involved the isolation of genomic DNA, theamplification of 16S rDNA gene using PCR the cloning of the gene using TOPO® TAcloning kit, restriction mapping of plasmid and sequencing of the gene by Sanger chain-terminationmethod. A total of 523 isolates were successfully purified (218 isolates fromSST and 305 isolates form FOR samples). Ten isolates exhibited antimicrobial activities(nine from SST samples and one from FOR samples). Four of these isolates wereidentified as Streptomyces, i.e. isolate SST2, No.2; SST4, No.11; SST5, No.14; andSST5, No.17. Further characterizations of the four showed they were different but stopshort at identifying the species of the Streptomyces. Further genotypic characterization ofisolate SST2, No.2 have shown that it has the 16S rDNA gene of 1576bp and similaritysearches lead to the Streptomyces sp. 10-3 and Streptomyces sp. 10-2. It can be concludedthat this project has achieved its main objectives while elucidating several new findingsfor future studies." @default.
- W2905308045 created "2018-12-22" @default.
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- W2905308045 date "2008-01-01" @default.
- W2905308045 modified "2023-09-24" @default.
- W2905308045 title "Isolation of streptomyces from Sabah and screening for their antimicrobial activities" @default.
- W2905308045 hasPublicationYear "2008" @default.
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