Matches in SemOpenAlex for { <https://semopenalex.org/work/W2905331359> ?p ?o ?g. }
- W2905331359 endingPage "339" @default.
- W2905331359 startingPage "324" @default.
- W2905331359 abstract "Granulosa cell (GC) proliferation is essential for follicular development. FSH is a key factor in GC proliferation, and a continuous supply of high levels of ATP is necessary for cell proliferation. However, genes encoding proteins of the glycolytic pathways are poorly expressed in GCs. Therefore, we hypothesized that mitochondrial gene expression and protein synthesis play a primary role in ATP production during GC proliferation. To test this hypothesis, we performed an in vivo study of GCs collected from 23-day-old mice ovaries with or without equine chorionic gonadotropin (eCG) priming. It was observed that mitochondrial activity with membrane potential, expression of protein-coding genes (Nd1-6, Cytb, Atpase6,8) and transcription-related genes (Polrmt, Tfam, Tfb2m), copy number of mitochondrial (mt-)DNA, and protein synthesis were increased in GCs after 24 hours of eCG injection and mostly maintained elevated up to 48 hours. Therefore, we performed in vitro culture of GCs in DMEM medium supplemented with FSH, testosterone, and serum and containing different glucose concentrations with or without d-chloramphenicol (CRP) for 24 hours. GC proliferation and ATP production were observed to be independent of glucose concentration. Furthermore, FSH-induced mitochondrial activity with membrane potential, ATP content, BrdU-incorporated cell proliferation, intensity of mt-ND1 and mt-ND6 proteins, and expressions of marker genes for proliferation and differentiation were significantly decreased by CRP treatment. These results revealed the crucial role of mitochondria in the supply of ATP and the necessity of mitochondrial gene expression and protein synthesis in not only the proliferation but also the differentiation of GCs during follicular development." @default.
- W2905331359 created "2018-12-22" @default.
- W2905331359 creator A5010372179 @default.
- W2905331359 creator A5012261150 @default.
- W2905331359 creator A5044990593 @default.
- W2905331359 creator A5090791363 @default.
- W2905331359 date "2018-12-10" @default.
- W2905331359 modified "2023-10-05" @default.
- W2905331359 title "Mitochondrial Protein Turnover Is Critical for Granulosa Cell Proliferation and Differentiation in Antral Follicles" @default.
- W2905331359 cites W111889214 @default.
- W2905331359 cites W1534478636 @default.
- W2905331359 cites W1751530372 @default.
- W2905331359 cites W1774855051 @default.
- W2905331359 cites W1804797980 @default.
- W2905331359 cites W1965105249 @default.
- W2905331359 cites W1966361056 @default.
- W2905331359 cites W1971932969 @default.
- W2905331359 cites W1985011732 @default.
- W2905331359 cites W1986050430 @default.
- W2905331359 cites W1997965154 @default.
- W2905331359 cites W2001830362 @default.
- W2905331359 cites W2014058353 @default.
- W2905331359 cites W2016738037 @default.
- W2905331359 cites W2016979866 @default.
- W2905331359 cites W2018504915 @default.
- W2905331359 cites W2023780628 @default.
- W2905331359 cites W2028092741 @default.
- W2905331359 cites W2036573675 @default.
- W2905331359 cites W2041067705 @default.
- W2905331359 cites W2046974635 @default.
- W2905331359 cites W2047028513 @default.
- W2905331359 cites W2052465225 @default.
- W2905331359 cites W2061320431 @default.
- W2905331359 cites W2065641902 @default.
- W2905331359 cites W2073883348 @default.
- W2905331359 cites W2076853816 @default.
- W2905331359 cites W2079717099 @default.
- W2905331359 cites W2089141103 @default.
- W2905331359 cites W2118796531 @default.
- W2905331359 cites W2119418077 @default.
- W2905331359 cites W2125287501 @default.
- W2905331359 cites W2128557475 @default.
- W2905331359 cites W2129482290 @default.
- W2905331359 cites W2130897208 @default.
- W2905331359 cites W2135104064 @default.
- W2905331359 cites W2142551110 @default.
- W2905331359 cites W2152684480 @default.
- W2905331359 cites W2157879044 @default.
- W2905331359 cites W2164225221 @default.
- W2905331359 cites W2173885591 @default.
- W2905331359 cites W2316494606 @default.
- W2905331359 cites W2559358005 @default.
- W2905331359 cites W2606123334 @default.
- W2905331359 cites W2747490774 @default.
- W2905331359 cites W2753268315 @default.
- W2905331359 cites W2767802610 @default.
- W2905331359 cites W2773927148 @default.
- W2905331359 cites W2789974331 @default.
- W2905331359 cites W2792406478 @default.
- W2905331359 cites W2794323391 @default.
- W2905331359 cites W2797133709 @default.
- W2905331359 doi "https://doi.org/10.1210/js.2018-00329" @default.
- W2905331359 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6330174" @default.
- W2905331359 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30652133" @default.
- W2905331359 hasPublicationYear "2018" @default.
- W2905331359 type Work @default.
- W2905331359 sameAs 2905331359 @default.
- W2905331359 citedByCount "21" @default.
- W2905331359 countsByYear W29053313592019 @default.
- W2905331359 countsByYear W29053313592020 @default.
- W2905331359 countsByYear W29053313592021 @default.
- W2905331359 countsByYear W29053313592022 @default.
- W2905331359 countsByYear W29053313592023 @default.
- W2905331359 crossrefType "journal-article" @default.
- W2905331359 hasAuthorship W2905331359A5010372179 @default.
- W2905331359 hasAuthorship W2905331359A5012261150 @default.
- W2905331359 hasAuthorship W2905331359A5044990593 @default.
- W2905331359 hasAuthorship W2905331359A5090791363 @default.
- W2905331359 hasBestOaLocation W29053313591 @default.
- W2905331359 hasConcept C104317684 @default.
- W2905331359 hasConcept C126322002 @default.
- W2905331359 hasConcept C134018914 @default.
- W2905331359 hasConcept C150194340 @default.
- W2905331359 hasConcept C153911025 @default.
- W2905331359 hasConcept C169415338 @default.
- W2905331359 hasConcept C187785154 @default.
- W2905331359 hasConcept C24586158 @default.
- W2905331359 hasConcept C2777203538 @default.
- W2905331359 hasConcept C2777229759 @default.
- W2905331359 hasConcept C28859421 @default.
- W2905331359 hasConcept C55493867 @default.
- W2905331359 hasConcept C62112901 @default.
- W2905331359 hasConcept C71924100 @default.
- W2905331359 hasConcept C75385678 @default.
- W2905331359 hasConcept C86803240 @default.
- W2905331359 hasConcept C95444343 @default.
- W2905331359 hasConceptScore W2905331359C104317684 @default.
- W2905331359 hasConceptScore W2905331359C126322002 @default.