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• W2905401980 abstract "The por1 gene encoding one of the mitochondrial porin channels in C. utilis CCTCC M 209298 was disrupted using a homologous recombination method. The co-production of S-adenosylmethionine (SAM) and glutathione (GSH) in the mutant C. utilis Δpor1 increased by 34.9% and 25.1%, respectively, during batch and fed-batch fermentation, relative to the parental strain. The average oxygen consumption rate, activities of key enzymes involved in SAM and GSH biosynthesis, levels of intracellular cofactors such as NADH and ATP, and carbon fluxes of key metabolites were compared between the parental strain and the Δpor1 mutant. The disruption of por1 gene increased the rate of mitochondrial respiration, increased the activities of both methionine adenosyltransferase and γ-glutamylcysteine synthetase, and enhanced the supply of energy and substrates for SAM and GSH biosynthesis, all of which favored the overproduction of SAM and GSH in the Δpor1 mutant." @default.
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• W2905401980 date "2019-01-01" @default.
• W2905401980 modified "2023-10-15" @default.
• W2905401980 title "Disruption of por1 gene in Candida utilis improves co-production of S-adenosylmethionine and glutathione" @default.
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• W2905401980 doi "https://doi.org/10.1016/j.jbiotec.2018.12.005" @default.
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