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• W2905402912 startingPage "1241" @default.
• W2905402912 abstract "The performance of photon-upconversion nanoparticles (UCNPs) as background-free luminescent labels in bioanalytical applications strongly depends on the preparation of well-defined and water-dispersible nanoconjugates. We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts of homogeneous UCNPs covered with carboxylated silica, biotin, or streptavidin with recovery rates of 30 to 50%. Clusters containing discrete numbers of UCNPs were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconversion microscopy, and additional complementary methods. The purified nanoconjugates improved conventional (analogue) bioaffinity assays and provided highly monodisperse conjugates for assays that rely on counting individual UCNPs (digital assays)." @default.
• W2905402912 created "2018-12-22" @default.
• W2905402912 creator A5020197475 @default.
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• W2905402912 date "2018-12-10" @default.
• W2905402912 modified "2023-10-09" @default.
• W2905402912 title "Large-Scale Purification of Photon-Upconversion Nanoparticles by Gel Electrophoresis for Analogue and Digital Bioassays" @default.
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• W2905402912 doi "https://doi.org/10.1021/acs.analchem.8b04488" @default.
• W2905402912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30525484" @default.
• W2905402912 hasPublicationYear "2018" @default.
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