Matches in SemOpenAlex for { <https://semopenalex.org/work/W2905606194> ?p ?o ?g. }
- W2905606194 endingPage "3366" @default.
- W2905606194 startingPage "3359" @default.
- W2905606194 abstract "Abstract BACKGROUND The salt tolerance of proteases secreted by Aspergillus oryzae 3.042 closely relates to the utilization of raw materials and the quality of soy sauce. However, little is known about the salt‐tolerant proteases and their salt‐tolerant mechanisms. RESULTS In this study, we isolated and identified a salt‐tolerant alkaline protease (AP, approximately 29 kDa) produced by A. oryzae 3.042. It was considered as a metal‐ion‐independent serine protease. The optimum and stable pH values were both pH 9.0 and the optimum temperature was 40 °C. Over 20% relative activity of AP remained in the presence of 3.0 mol L −1 NaCl after 7 days, but its K m and V max were only mildly influenced by the presence of 3.0 mol L −1 NaCl, indicating its outstanding salt tolerance. Furthermore, AP was more stable than non‐salt‐tolerant protease at high salinity. The salt‐tolerant mechanisms of AP could be due to more salt bridges, higher proportion of ordered secondary structures and stronger hydrophobic amino acid residues in the interior. CONCLUSIONS The above results are vital for maintaining, activating and/or modulating the activity of AP in high‐salt environments. They would also provide theoretical guidance for the modification of AP and the engineering of A. oryzae 3.042 so as to secrete more AP. © 2018 Society of Chemical Industry" @default.
- W2905606194 created "2019-01-01" @default.
- W2905606194 creator A5005027702 @default.
- W2905606194 creator A5016026704 @default.
- W2905606194 creator A5029916443 @default.
- W2905606194 creator A5062051935 @default.
- W2905606194 creator A5079080440 @default.
- W2905606194 creator A5080115371 @default.
- W2905606194 date "2019-02-19" @default.
- W2905606194 modified "2023-10-15" @default.
- W2905606194 title "Separation, biochemical characterization and salt‐tolerant mechanisms of alkaline protease from <i>Aspergillus oryzae</i>" @default.
- W2905606194 cites W1961156066 @default.
- W2905606194 cites W1998261106 @default.
- W2905606194 cites W2005110802 @default.
- W2905606194 cites W2029667189 @default.
- W2905606194 cites W2047605881 @default.
- W2905606194 cites W2047841570 @default.
- W2905606194 cites W2048631735 @default.
- W2905606194 cites W2053669884 @default.
- W2905606194 cites W2061138809 @default.
- W2905606194 cites W2074405611 @default.
- W2905606194 cites W2077953924 @default.
- W2905606194 cites W2086136843 @default.
- W2905606194 cites W2092057183 @default.
- W2905606194 cites W2121208681 @default.
- W2905606194 cites W2139119119 @default.
- W2905606194 cites W2150981663 @default.
- W2905606194 cites W2163553212 @default.
- W2905606194 cites W2304152817 @default.
- W2905606194 cites W2316253106 @default.
- W2905606194 cites W2410578887 @default.
- W2905606194 cites W2724306071 @default.
- W2905606194 cites W2736712539 @default.
- W2905606194 cites W2744639621 @default.
- W2905606194 cites W2757939760 @default.
- W2905606194 cites W2772295647 @default.
- W2905606194 cites W2806375834 @default.
- W2905606194 cites W4211254213 @default.
- W2905606194 cites W4293247451 @default.
- W2905606194 doi "https://doi.org/10.1002/jsfa.9553" @default.
- W2905606194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30584796" @default.
- W2905606194 hasPublicationYear "2019" @default.
- W2905606194 type Work @default.
- W2905606194 sameAs 2905606194 @default.
- W2905606194 citedByCount "26" @default.
- W2905606194 countsByYear W29056061942019 @default.
- W2905606194 countsByYear W29056061942020 @default.
- W2905606194 countsByYear W29056061942021 @default.
- W2905606194 countsByYear W29056061942022 @default.
- W2905606194 countsByYear W29056061942023 @default.
- W2905606194 crossrefType "journal-article" @default.
- W2905606194 hasAuthorship W2905606194A5005027702 @default.
- W2905606194 hasAuthorship W2905606194A5016026704 @default.
- W2905606194 hasAuthorship W2905606194A5029916443 @default.
- W2905606194 hasAuthorship W2905606194A5062051935 @default.
- W2905606194 hasAuthorship W2905606194A5079080440 @default.
- W2905606194 hasAuthorship W2905606194A5080115371 @default.
- W2905606194 hasConcept C129513315 @default.
- W2905606194 hasConcept C178790620 @default.
- W2905606194 hasConcept C181199279 @default.
- W2905606194 hasConcept C182220744 @default.
- W2905606194 hasConcept C185592680 @default.
- W2905606194 hasConcept C18903297 @default.
- W2905606194 hasConcept C2776371256 @default.
- W2905606194 hasConcept C2776714187 @default.
- W2905606194 hasConcept C2777807008 @default.
- W2905606194 hasConcept C2779175654 @default.
- W2905606194 hasConcept C2781204770 @default.
- W2905606194 hasConcept C2992246531 @default.
- W2905606194 hasConcept C31903555 @default.
- W2905606194 hasConcept C55493867 @default.
- W2905606194 hasConcept C59822182 @default.
- W2905606194 hasConcept C86803240 @default.
- W2905606194 hasConceptScore W2905606194C129513315 @default.
- W2905606194 hasConceptScore W2905606194C178790620 @default.
- W2905606194 hasConceptScore W2905606194C181199279 @default.
- W2905606194 hasConceptScore W2905606194C182220744 @default.
- W2905606194 hasConceptScore W2905606194C185592680 @default.
- W2905606194 hasConceptScore W2905606194C18903297 @default.
- W2905606194 hasConceptScore W2905606194C2776371256 @default.
- W2905606194 hasConceptScore W2905606194C2776714187 @default.
- W2905606194 hasConceptScore W2905606194C2777807008 @default.
- W2905606194 hasConceptScore W2905606194C2779175654 @default.
- W2905606194 hasConceptScore W2905606194C2781204770 @default.
- W2905606194 hasConceptScore W2905606194C2992246531 @default.
- W2905606194 hasConceptScore W2905606194C31903555 @default.
- W2905606194 hasConceptScore W2905606194C55493867 @default.
- W2905606194 hasConceptScore W2905606194C59822182 @default.
- W2905606194 hasConceptScore W2905606194C86803240 @default.
- W2905606194 hasFunder F4320321001 @default.
- W2905606194 hasFunder F4320321410 @default.
- W2905606194 hasFunder F4320321543 @default.
- W2905606194 hasFunder F4320325437 @default.
- W2905606194 hasFunder F4320326182 @default.
- W2905606194 hasIssue "7" @default.
- W2905606194 hasLocation W29056061941 @default.
- W2905606194 hasLocation W29056061942 @default.
- W2905606194 hasOpenAccess W2905606194 @default.