Matches in SemOpenAlex for { <https://semopenalex.org/work/W2905663423> ?p ?o ?g. }
- W2905663423 endingPage "e0209506" @default.
- W2905663423 startingPage "e0209506" @default.
- W2905663423 abstract "Microbial rhodopsins have become an important tool in the field of optogenetics. However, effective in vivo optogenetics is in many cases severely limited due to the strong absorption and scattering of visible light by biological tissues. Recently, a combination of opsin site-directed mutagenesis and analog retinal substitution has produced variants of proteorhodopsin which absorb maximally in the near-infrared (NIR). In this study, UV-Visible-NIR absorption and resonance Raman spectroscopy were used to study the double mutant, D212N/F234S, of green absorbing proteorhodopsin (GPR) regenerated with MMAR, a retinal analog containing a methylamino modified β-ionone ring. Four distinct subcomponent absorption bands with peak maxima near 560, 620, 710 and 780 nm are detected with the NIR bands dominant at pH <7.3, and the visible bands dominant at pH 9.5. FT-Raman using 1064-nm excitation reveal two strong ethylenic bands at 1482 and 1498 cm-1 corresponding to the NIR subcomponent absorption bands based on an extended linear correlation between λmax and γC = C. This spectrum exhibits two intense bands in the fingerprint and HOOP mode regions that are highly characteristic of the O640 photointermediate from the light-adapted bacteriorhodopsin photocycle. In contrast, 532-nm excitation enhances the 560-nm component, which exhibits bands very similar to light-adapted bacteriorhodopsin and/or the acid-purple form of bacteriorhodopsin. Native GPR and its mutant D97N when regenerated with MMAR also exhibit similar absorption and Raman bands but with weaker contributions from the NIR absorbing components. Based on these results it is proposed that the NIR absorption in GPR-D212N/F234S with MMAR arises from an O-like chromophore, where the Schiff base counterion D97 is protonated and the MMAR adopts an all-trans configuration with a non-planar geometry due to twists in the conjugated polyene segment. This configuration is characterized by extensive charge delocalization, most likely involving nitrogens atoms in the MMAR chromophore." @default.
- W2905663423 created "2019-01-01" @default.
- W2905663423 creator A5001534806 @default.
- W2905663423 creator A5005249262 @default.
- W2905663423 creator A5018343968 @default.
- W2905663423 creator A5041262815 @default.
- W2905663423 creator A5042428472 @default.
- W2905663423 creator A5049078993 @default.
- W2905663423 date "2018-12-26" @default.
- W2905663423 modified "2023-10-16" @default.
- W2905663423 title "Raman spectroscopy of a near infrared absorbing proteorhodopsin: Similarities to the bacteriorhodopsin O photointermediate" @default.
- W2905663423 cites W1487848365 @default.
- W2905663423 cites W1491956303 @default.
- W2905663423 cites W1497743473 @default.
- W2905663423 cites W1509410086 @default.
- W2905663423 cites W1510749468 @default.
- W2905663423 cites W1540030652 @default.
- W2905663423 cites W1604437134 @default.
- W2905663423 cites W1758338034 @default.
- W2905663423 cites W1824878861 @default.
- W2905663423 cites W1826482223 @default.
- W2905663423 cites W1963944233 @default.
- W2905663423 cites W1965282035 @default.
- W2905663423 cites W1965474683 @default.
- W2905663423 cites W1971950456 @default.
- W2905663423 cites W1973003055 @default.
- W2905663423 cites W1973221059 @default.
- W2905663423 cites W1973299216 @default.
- W2905663423 cites W1973334425 @default.
- W2905663423 cites W1979283946 @default.
- W2905663423 cites W1981322684 @default.
- W2905663423 cites W1986501700 @default.
- W2905663423 cites W1990622776 @default.
- W2905663423 cites W1991020048 @default.
- W2905663423 cites W1992496696 @default.
- W2905663423 cites W1994647098 @default.
- W2905663423 cites W1996468364 @default.
- W2905663423 cites W1997562615 @default.
- W2905663423 cites W2000485299 @default.
- W2905663423 cites W2005286485 @default.
- W2905663423 cites W2008401426 @default.
- W2905663423 cites W2010207349 @default.
- W2905663423 cites W2016716827 @default.
- W2905663423 cites W2017601133 @default.
- W2905663423 cites W2023178626 @default.
- W2905663423 cites W2023416900 @default.
- W2905663423 cites W2024672292 @default.
- W2905663423 cites W2028410153 @default.
- W2905663423 cites W2028723563 @default.
- W2905663423 cites W2031019230 @default.
- W2905663423 cites W2031210068 @default.
- W2905663423 cites W2031276816 @default.
- W2905663423 cites W2031391041 @default.
- W2905663423 cites W2036536328 @default.
- W2905663423 cites W2037170914 @default.
- W2905663423 cites W2040211368 @default.
- W2905663423 cites W2041605941 @default.
- W2905663423 cites W2054001378 @default.
- W2905663423 cites W2056534060 @default.
- W2905663423 cites W2058634232 @default.
- W2905663423 cites W2059997873 @default.
- W2905663423 cites W2060153272 @default.
- W2905663423 cites W2062423705 @default.
- W2905663423 cites W2062440132 @default.
- W2905663423 cites W2062704670 @default.
- W2905663423 cites W2066024697 @default.
- W2905663423 cites W2070042548 @default.
- W2905663423 cites W2072336276 @default.
- W2905663423 cites W2072590703 @default.
- W2905663423 cites W2073204622 @default.
- W2905663423 cites W2074337748 @default.
- W2905663423 cites W2085073403 @default.
- W2905663423 cites W2093562743 @default.
- W2905663423 cites W2094346915 @default.
- W2905663423 cites W2094968175 @default.
- W2905663423 cites W2096387004 @default.
- W2905663423 cites W2108410757 @default.
- W2905663423 cites W2115138779 @default.
- W2905663423 cites W2115357061 @default.
- W2905663423 cites W2136613480 @default.
- W2905663423 cites W2139197470 @default.
- W2905663423 cites W2145909957 @default.
- W2905663423 cites W2151300356 @default.
- W2905663423 cites W2157371027 @default.
- W2905663423 cites W2160564267 @default.
- W2905663423 cites W2160779028 @default.
- W2905663423 cites W2167495642 @default.
- W2905663423 cites W2256174486 @default.
- W2905663423 cites W2319875947 @default.
- W2905663423 cites W2321566341 @default.
- W2905663423 cites W2326312316 @default.
- W2905663423 cites W2556837770 @default.
- W2905663423 cites W2563293115 @default.
- W2905663423 cites W2574116968 @default.
- W2905663423 cites W2594189097 @default.
- W2905663423 cites W2605999716 @default.
- W2905663423 cites W2607026522 @default.
- W2905663423 cites W2610074214 @default.