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- W2905904433 abstract "Measuring functional opsonic antibodies against group A streptococci is an important component of the clinical development path for effective vaccines. Prior studies have used an assay developed over 60 years ago that relied on whole human blood as the source of phagocytes and complement, both of which are critical components of antibody-mediated killing assays. In this study, we adapted an assay that uses the HL-60 human promyelocytic leukemia cell line as phagocytic cells and baby rabbit serum as a source of complement for detection of opsonic antibodies against group A streptococci. On the basis of some of the known biological characteristics of the bacteria, we modified the assay conditions to support the evaluation of 21 epidemiologically important M types and demonstrated the utility and reproducibility of the assay for measurement of functional opsonic antibody levels." @default.
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- W2905904433 date "2018-12-26" @default.
- W2905904433 modified "2023-10-16" @default.
- W2905904433 title "Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against <i>Streptococcus pyogenes</i>" @default.
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- W2905904433 doi "https://doi.org/10.1128/msphere.00617-18" @default.
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