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- W2905928168 abstract "Introduction: In evaluation of undiagnosed pleural effusion, diagnosis of pleural tuberculosis remains a challenge because of low yield of conventional methods. Pleural biopsy is the gold standard for confirmation of diagnosis. Since mycobacterial peptide specific activated lymphocytes are likely to be present at the site of infection, we evaluated the performance of Enzyme-linked Immunospot (ELISPOT) in pleural fluid for the diagnosis of pleural tuberculosis in histopathologically confirmed cases. Methods: Forty two consecutive cases of undiagnosed pleural effusion were subjected to thoracoscopy guided pleural biopsy. Thirteen patients were confirmed to have tuberculosis, 27 had malignancy and 2 had normal pleura. A total of 1 x 103 pleural fluid mononuclear cells were cultured in the presence of early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) for 24 hours. The individual spots were then counted using an automated image analysis system ELISPOT reader. Result: The number of spots developed in pleural fluid were significantly higher in tubercular pleural effusions as compared to non-tubercular effusions (CFP-10: 154.76±14.61 vs 49.24±8.9, ESAT-6: 150.3±17.27 v/s 45.34±8.23, p Conclusion: ELISPOT can be a useful non-invasive test for evaluation of undiagnosed pleural effusion which can confirm or rule out tuberculosis with confidence." @default.
- W2905928168 created "2019-01-01" @default.
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- W2905928168 date "2018-09-15" @default.
- W2905928168 modified "2023-10-17" @default.
- W2905928168 title "Use of pleural fluid interferon-gamma enzyme-linked immunospot (ELISPOT) assay in the evaluation of undiagnosed pleural effusion" @default.
- W2905928168 doi "https://doi.org/10.1183/13993003.congress-2018.pa2760" @default.
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