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- W2905947577 abstract "The development of plant breeding in vitro is a solution to increase sugarcane production. In addition, cytology studies and the proliferation of embryogenic callus plays an important role in the success of the genetic transformation process. An important stage in the induction of somatic embryogenesis is to ensure that the resulting callus is completely embryogenic in that it can induce the formation of somatic embryos derived from a single cell. Therefore, cytological studies should be undertaken to see the development stage of embryogenic callus embryos produced. This study aims to examine the important stages of somatic embryogenesis from the process of single cell differentiation to formation of plantlet, so it is expected to facilitate the use and understanding of somatic embryo process and in the preparation of strategic research related to the process of genetic transformation. Microscopic observations showed that the resulting callus was a mixture of embryogenic and non-embryogenic cells. The resulting embryogenic cell group is considered a pre-embryogenic mass (PEM). This study found important mechanisms during the process of somatic embryogenesis that were asymmetric cell division and cell lengthening due to the use of 2,4-D and casein hydrolysate as a source of nitrogen." @default.
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- W2905947577 date "2018-06-29" @default.
- W2905947577 modified "2023-09-24" @default.
- W2905947577 title "Embryogenic Callus Cytology and Regeneration Condition for Genetic Transformation of Sugarcane" @default.
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