FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2906103281> ?p ?o ?g. }

• W2906103281 endingPage "523" @default.
• W2906103281 startingPage "506" @default.
• W2906103281 abstract "Activation of the epidermal growth factor (EGF) receptor (EGFR) at the cell surface initiates signaling through the RAS-RAF-MAPK/ERK1/2 pathway and receptor endocytosis. Whether this signaling continues from endosomes remains unclear, because RAS is predominantly located on the plasma membrane, and the localization of endogenous RAF kinases, downstream effectors of RAS, is not defined. To examine RAF localization, we labeled endogenous RAF1 with mVenus using gene editing. From 10 to 15% of RAF1-mVenus (<2000 molecules/cell), which was initially entirely cytosolic, transiently translocated to the plasma membrane after EGF stimulation. Following an early burst of translocation, the membrane-associated RAF1-mVenus was undetectable by microscopy or subcellular fractionation, and this pool was estimated to be <200 molecules per cell. In contrast, persistent EGF-dependent translocation of RAF1-mVenus to the plasma membrane was driven by the RAF inhibitor sorafenib, which increases the affinity of Ras-GTP:RAF1 interactions. RAF1-mVenus was not found in EGFR-containing endosomes under any conditions. Computational modeling of RAF1 dynamics revealed that RAF1 membrane abundance is controlled most prominently by association and dissociation rates from RAS-GTP and by RAS-GTP concentration. The model further suggested that the relatively protracted activation of the RAF-MEK1/2-ERK1/2 module, in comparison with RAF1 membrane localization, may involve multiple rounds of cytosolic RAF1 rebinding to active RAS at the membrane." @default.
• W2906103281 created "2019-01-01" @default.
• W2906103281 creator A5065267926 @default.
• W2906103281 creator A5069378198 @default.
• W2906103281 creator A5073726679 @default.
• W2906103281 creator A5075470291 @default.
• W2906103281 creator A5088924932 @default.
• W2906103281 creator A5089369638 @default.
• W2906103281 date "2019-02-15" @default.
• W2906103281 modified "2023-09-27" @default.
• W2906103281 title "Localization dynamics of endogenous fluorescently labeled RAF1 in EGF-stimulated cells" @default.
• W2906103281 cites W1535997186 @default.
• W2906103281 cites W1705959389 @default.
• W2906103281 cites W1896838955 @default.
• W2906103281 cites W19005219 @default.
• W2906103281 cites W1965458495 @default.
• W2906103281 cites W1972399884 @default.
• W2906103281 cites W1975206919 @default.
• W2906103281 cites W1977709885 @default.
• W2906103281 cites W1978760124 @default.
• W2906103281 cites W1984536659 @default.
• W2906103281 cites W1984906223 @default.
• W2906103281 cites W1985309852 @default.
• W2906103281 cites W1989875198 @default.
• W2906103281 cites W1995624041 @default.
• W2906103281 cites W2000581759 @default.
• W2906103281 cites W2004295816 @default.
• W2906103281 cites W2006098171 @default.
• W2906103281 cites W2006882600 @default.
• W2906103281 cites W2011780255 @default.
• W2906103281 cites W2033185023 @default.
• W2906103281 cites W2034036293 @default.
• W2906103281 cites W2035064405 @default.
• W2906103281 cites W2035087170 @default.
• W2906103281 cites W2050568471 @default.
• W2906103281 cites W2050573392 @default.
• W2906103281 cites W2051165598 @default.
• W2906103281 cites W2052811553 @default.
• W2906103281 cites W2054946171 @default.
• W2906103281 cites W2056787790 @default.
• W2906103281 cites W2064850685 @default.
• W2906103281 cites W2067005574 @default.
• W2906103281 cites W2074631079 @default.
• W2906103281 cites W2079398727 @default.
• W2906103281 cites W2080047102 @default.
• W2906103281 cites W2082507728 @default.
• W2906103281 cites W2096595244 @default.
• W2906103281 cites W2105467845 @default.
• W2906103281 cites W2107495112 @default.
• W2906103281 cites W2107578201 @default.
• W2906103281 cites W2107921791 @default.
• W2906103281 cites W2117669008 @default.
• W2906103281 cites W2123541095 @default.
• W2906103281 cites W2136209368 @default.
• W2906103281 cites W2137715268 @default.
• W2906103281 cites W2140475345 @default.
• W2906103281 cites W2140765540 @default.
• W2906103281 cites W2145950214 @default.
• W2906103281 cites W2149662085 @default.
• W2906103281 cites W2160711324 @default.
• W2906103281 cites W2165685835 @default.
• W2906103281 cites W2167197258 @default.
• W2906103281 cites W2168887892 @default.
• W2906103281 cites W2253412135 @default.
• W2906103281 cites W2465367340 @default.
• W2906103281 cites W2474410556 @default.
• W2906103281 cites W2594443977 @default.
• W2906103281 cites W2767191415 @default.
• W2906103281 cites W2950427239 @default.
• W2906103281 doi "https://doi.org/10.1091/mbc.e18-08-0512" @default.
• W2906103281 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6594441" @default.
• W2906103281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30586319" @default.
• W2906103281 hasPublicationYear "2019" @default.
• W2906103281 type Work @default.
• W2906103281 sameAs 2906103281 @default.
• W2906103281 citedByCount "10" @default.
• W2906103281 countsByYear W29061032812021 @default.
• W2906103281 countsByYear W29061032812022 @default.
• W2906103281 countsByYear W29061032812023 @default.
• W2906103281 crossrefType "journal-article" @default.
• W2906103281 hasAuthorship W2906103281A5065267926 @default.
• W2906103281 hasAuthorship W2906103281A5069378198 @default.
• W2906103281 hasAuthorship W2906103281A5073726679 @default.
• W2906103281 hasAuthorship W2906103281A5075470291 @default.
• W2906103281 hasAuthorship W2906103281A5088924932 @default.
• W2906103281 hasAuthorship W2906103281A5089369638 @default.
• W2906103281 hasBestOaLocation W29061032811 @default.
• W2906103281 hasConcept C102747710 @default.
• W2906103281 hasConcept C1491633281 @default.
• W2906103281 hasConcept C16613235 @default.
• W2906103281 hasConcept C170493617 @default.
• W2906103281 hasConcept C181199279 @default.
• W2906103281 hasConcept C27239569 @default.
• W2906103281 hasConcept C2776362946 @default.
• W2906103281 hasConcept C2778106830 @default.
• W2906103281 hasConcept C28005876 @default.
• W2906103281 hasConcept C41625074 @default.
• W2906103281 hasConcept C55493867 @default.

• next