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• W2906255712 abstract "A novel NH4+ voltammetric electrochemical biosensor was constructed by immobilizing glutamate dehydrogenase (GLDH)/Fe3O4/graphene (GR)/chitosan (CS) nanobiocomposite onto a glassy carbon electrode (GCE). On the GLDH/Fe3O4/GR/CS/GCE, GLDH catalyzed the reversible reaction, i.e., the reductive amination of α-ketoglutaric acid and the oxidative deamination of L-glutamate. The electrons produced in the enzymatic reactions were transferred to the surface of the electrode via the [Fe(CN)6]3−/4− couple, which helped for the amplification of the electrochemical signal. The electrochemical detection of NH4+ was based on the fact that the enhanced response current was proportional to the NH4+ concentration. Owing to the combination of the advantages of the synergistic effects of Fe3O4 nanospheres, GR and CS, a promising platform for NH4+ sensing was provided. Under optimum conditions, the introduced biosensor had a linear range of 0.4–2.0 μM for NH4+ with the detection and quantification limits of 0.08 and 0.27 μM, respectively. Moreover, the biosensor exhibited good sensitivity and excellent reproducibility. It could retain 91.8% of its original response after two weeks of storage at 4 °C, suggesting satisfactory stability. Additionally, the proposed biosensor was successfully applied to detect NH4+ levels in PM2.5 samples, indicating its feasibility for application in NH4+monitoring in the environmental fields." @default.
• W2906255712 created "2019-01-01" @default.
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• W2906255712 date "2019-05-01" @default.
• W2906255712 modified "2023-10-17" @default.
• W2906255712 title "A promising voltammetric biosensor based on glutamate dehydrogenase/Fe3O4/graphene/chitosan nanobiocomposite for sensitive ammonium determination in PM2.5" @default.
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• W2906255712 doi "https://doi.org/10.1016/j.talanta.2018.12.090" @default.
• W2906255712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30771985" @default.
• W2906255712 hasPublicationYear "2019" @default.
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