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- W2906460905 abstract "3D single-molecule localization microscopy relies on fitting the shape of point-spread-functions (PSFs) recorded on a wide-field detector. However, optical aberrations distort those shapes, which compromises the accuracy and precision of single-molecule localization microscopy. Here, we employ a computational phase retrieval based on a vectorial PSF model to quantify the spatial variance of optical aberrations in a two-channel ultrawide-field single-molecule localization microscope. The use of a spatially variant PSF model enables accurate and precise emitter localization in x-, y- and z-directions throughout the entire field of view." @default.
- W2906460905 created "2019-01-01" @default.
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- W2906460905 date "2019-04-19" @default.
- W2906460905 modified "2023-10-16" @default.
- W2906460905 title "Computational correction of spatially variant optical aberrations in 3D single-molecule localization microscopy" @default.
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- W2906460905 doi "https://doi.org/10.1364/oe.27.012582" @default.
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