FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2906495118> ?p ?o ?g. }

• W2906495118 abstract "Developmental biology studies the processes by which a cell divides and differentiates to generate a full functional organism.Up to the onset of gastrulation, cells from the embryo remain pluripotent, but precisely at this stage they differentiate towards mesoderm, endoderm, ectoderm and primordial germ cells. Pluripotency factors maintain uncommitted cells of the blastocyst and embryonic stem cells in culture in the pluripotent state. However, little is known about the role played by these factors during later development, despite their being expressed in the postimplantation epiblast. At this stage, progenitors of the first hematopoietic cells in the mouse arise in the early mesodermal progenitors in the posterior-proximal region of the epiblast, but the mechanisms that specify primitive blood cells are still largely unknown. At the same time, the embryo polarizes and asymmetry arises with the formation of the anterior-posterior axis.Using a dual transgene system for controlled expression at postimplantation stages, we found that NANOG, a pluripotency factor crucial for pluripotency maintenance, blocks primitive hematopoiesis in the gastrulating embryo, resulting in a loss of red blood cells and downregulation of erythropoietic genes. Accordingly, Nanog deficient embryonic stem cells are prone to erythropoietic differentiation. Moreover, Nanog expression in adults prevents the maturation of erythroid cells. By analysis of available data for NANOG binding during stem cell differentiation and CRISPR/Cas9 genome editing, we found that Tal1 is a direct target of NANOG.We also found that Nanog represses anteriorization of the epiblast. By analyzing NanogKO ES cell RNAseq during naive to primed transition and single cell RNAseq of gastrulating mouse embryos, we discovered that Nanog directly downregulates Pou3f1, that codes for a transcription factor important for anteriorization and neural development. Our results show that Nanog exerts crucial functions at the exit of pluripotency, and that it regulates primitive hematopoiesis and anteriorization of the embryo by directly repressing critical lineage specifiers." @default.
• W2906495118 created "2019-01-01" @default.
• W2906495118 creator A5059554444 @default.
• W2906495118 date "2018-07-13" @default.
• W2906495118 modified "2023-09-27" @default.
• W2906495118 title "NANOG at the exit of pluripotency: new roles in the gastrulating mouse embryo" @default.
• W2906495118 cites W147863779 @default.
• W2906495118 cites W1484163259 @default.
• W2906495118 cites W1608762409 @default.
• W2906495118 cites W1664587663 @default.
• W2906495118 cites W1829700783 @default.
• W2906495118 cites W1859466979 @default.
• W2906495118 cites W1874849227 @default.
• W2906495118 cites W1887248883 @default.
• W2906495118 cites W1927685549 @default.
• W2906495118 cites W1937332911 @default.
• W2906495118 cites W1957763880 @default.
• W2906495118 cites W1964081206 @default.
• W2906495118 cites W1965258554 @default.
• W2906495118 cites W1965716494 @default.
• W2906495118 cites W1966758700 @default.
• W2906495118 cites W1968721773 @default.
• W2906495118 cites W1969589057 @default.
• W2906495118 cites W1970051600 @default.
• W2906495118 cites W1973387539 @default.
• W2906495118 cites W1974365957 @default.
• W2906495118 cites W1974712344 @default.
• W2906495118 cites W1975736038 @default.
• W2906495118 cites W1976337831 @default.
• W2906495118 cites W1978031011 @default.
• W2906495118 cites W1981088384 @default.
• W2906495118 cites W1983146536 @default.
• W2906495118 cites W1985360296 @default.
• W2906495118 cites W1986227171 @default.
• W2906495118 cites W1988161053 @default.
• W2906495118 cites W1988294855 @default.
• W2906495118 cites W1988397986 @default.
• W2906495118 cites W1988571169 @default.
• W2906495118 cites W1990565971 @default.
• W2906495118 cites W1996300461 @default.
• W2906495118 cites W1997771807 @default.
• W2906495118 cites W1999047431 @default.
• W2906495118 cites W1999574084 @default.
• W2906495118 cites W2000292621 @default.
• W2906495118 cites W2000466286 @default.
• W2906495118 cites W2001429836 @default.
• W2906495118 cites W2003336545 @default.
• W2906495118 cites W2004196170 @default.
• W2906495118 cites W2006417453 @default.
• W2906495118 cites W2008558812 @default.
• W2906495118 cites W2016060629 @default.
• W2906495118 cites W2016680950 @default.
• W2906495118 cites W2017124906 @default.
• W2906495118 cites W2023262022 @default.
• W2906495118 cites W2023745283 @default.
• W2906495118 cites W2030535403 @default.
• W2906495118 cites W2031683488 @default.
• W2906495118 cites W2034609545 @default.
• W2906495118 cites W2035222904 @default.
• W2906495118 cites W2036530128 @default.
• W2906495118 cites W2036897871 @default.
• W2906495118 cites W2040576499 @default.
• W2906495118 cites W2041352113 @default.
• W2906495118 cites W2042982622 @default.
• W2906495118 cites W2044830131 @default.
• W2906495118 cites W2046516822 @default.
• W2906495118 cites W2048108183 @default.
• W2906495118 cites W2049595902 @default.
• W2906495118 cites W2051740914 @default.
• W2906495118 cites W2053482584 @default.
• W2906495118 cites W2053490054 @default.
• W2906495118 cites W2054911279 @default.
• W2906495118 cites W2057159461 @default.
• W2906495118 cites W2057375692 @default.
• W2906495118 cites W2065746091 @default.
• W2906495118 cites W2066972114 @default.
• W2906495118 cites W2067505689 @default.
• W2906495118 cites W2068780842 @default.
• W2906495118 cites W2070451931 @default.
• W2906495118 cites W2070700342 @default.
• W2906495118 cites W2072818545 @default.
• W2906495118 cites W2076153728 @default.
• W2906495118 cites W2077765163 @default.
• W2906495118 cites W2078997694 @default.
• W2906495118 cites W2081581923 @default.
• W2906495118 cites W2082414007 @default.
• W2906495118 cites W2086370314 @default.
• W2906495118 cites W2086979403 @default.
• W2906495118 cites W2087004817 @default.
• W2906495118 cites W2087634817 @default.
• W2906495118 cites W2088128367 @default.
• W2906495118 cites W2088257765 @default.
• W2906495118 cites W2088473598 @default.
• W2906495118 cites W2090260381 @default.
• W2906495118 cites W2091571816 @default.
• W2906495118 cites W2093339798 @default.
• W2906495118 cites W2100825889 @default.
• W2906495118 cites W2102235440 @default.
• W2906495118 cites W2103790825 @default.
• W2906495118 cites W2107665951 @default.

• next