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• W2906528872 startingPage "356" @default.
• W2906528872 abstract "Melittin is an extensively studied, 26-residue toxic peptide from honey bee venom. Because of its versatility in adopting a variety of secondary (helix or coil) and quaternary (monomer or tetramer) structures in various environments, melittin has been the focus of numerous investigations as a model peptide in protein folding studies as well as in studies involving binding to proteins, lipids, and polysaccharides. A significant body of evidence supports the view that melittin binds to these macromolecules in a predominantly helical conformation, but detailed structural knowledge of this conformation is lacking. In this report, we present nuclear magnetic resonance (NMR)-based structural insights into the helix formation of recombinant melittin in the presence of trifluoroethanol (TFE): a known secondary structure inducer in peptides. These studies were performed at neutral pH, with micromolar amounts of the peptide. Using nuclear Overhauser effect (NOE)-derived distance restraints from three-dimensional NMR spectra, we determined the atomic resolution solution NMR structure of recombinant melittin bearing a TFE-stabilized helix. To circumvent the complications with structure determination of small peptides with high conformational flexibility, we developed a workflow for enhancing proton NOEs by increasing the viscosity of the medium. In the TFE-containing medium, recombinant monomeric melittin forms a long, continuous helical structure, which consists of the N- and C-terminal α-helices and the noncanonical 310-helix in the middle. The noncanonical 310-helix is missing in the previously solved X-ray structure of tetrameric melittin and the NMR structure of melittin in methanol. Melittin's structure in TFE-containing medium provides insights into melittin's conformational transitions, which are relevant to the peptide's interactions with its biological targets." @default.
• W2906528872 created "2019-01-01" @default.
• W2906528872 creator A5042490894 @default.
• W2906528872 creator A5049479890 @default.
• W2906528872 creator A5084202200 @default.
• W2906528872 date "2018-12-20" @default.
• W2906528872 modified "2023-10-17" @default.
• W2906528872 title "Helical Structure of Recombinant Melittin" @default.
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• W2906528872 doi "https://doi.org/10.1021/acs.jpcb.8b08424" @default.
• W2906528872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6465175" @default.
• W2906528872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30570258" @default.
• W2906528872 hasPublicationYear "2018" @default.
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