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- W2906770339 abstract "The number of bacterial strains that are resistant to multiple conventional antimicrobial agents is increasing. In this context, natural products have been widely used as a strategy to treat diseases caused by bacteria. Infections by Helicobacter pylori have attracted attention because they are directly related to severe gastric medical conditions. Casearia sylvestris Swartz, popularly known as guaçatonga, is largely employed to treat gastric disorders in Brazilian folk medicine. This plant species has aroused much interest mainly because it displays anti-inflammatory activity and can act as an antiulcer agent.To evaluate the in vitro and in vivo anti-H. pylori action of C. sylvestris leaf derivatives incorporated or not in a nanostructured drug delivery system.The essential oil (obtained by hydrodistillation) and ethanolic extract (obtained by maceration) were obtained from C. sylvestris leaves. The ethanolic extract was submitted to fractionation through solid phase extraction and column chromatography, to yield the ethanolic fractions. Hydrolyzed casearin J was achieved by submitting isolated casearin J to acid hydrolysis. The derivatives were chemically characterized by nuclear magnetic resonance (NMR), gas chromatography (GC), and gas chromatography-mass spectrometry (GC-MS) analyses. A nanostructured lipid system was used as drug delivery system. To assess the in vitro antibacterial activity of C. sylvestris leaf essential oil, ethanolic extract, and derivatives, microdilution, biofilm, and time-kill assays were performed against H. pylori ATCC 43504. Finally, the in vivo action was investigated by employing male Wistar rats experimentally infected with H. pylori.Many C. sylvestris leaf derivatives presented significant in vitro activity against H. pylori. Among the derivatives, fraction 2 (F2) was the most effective. In vivo tests showed that both the ethanolic extract and F2 decreased the ulcerative lesion size, but only the ethanolic extract eradicated H. pylori from the gastric lesions. Incorporation of plant derivatives in nanostructured lipid system blunted the in vitro action, as demonstrated by the microdilution assay. However, this incorporation improved the ethanolic extract activity against biofilms.C. sylvestris leaf derivatives are effective against H. pylori both in vitro and in vivo. According to phytochemical analyses, these derivatives are rich in terpenoids, which could be related to the anti-H. pylori action. Synergism could also underlie C. sylvestris efficacy judging from the fact that the sub-fractions and isolated compounds had lower activity than the extract. Incorporation in a nanostructured lipid system did not improve the activity of the compounds in our in vivo protocol." @default.
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- W2906770339 date "2019-04-01" @default.
- W2906770339 modified "2023-10-12" @default.
- W2906770339 title "In vitro and in vivo anti-Helicobacter pylori activity of Casearia sylvestris leaf derivatives" @default.
- W2906770339 cites W1552337304 @default.
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- W2906770339 cites W1597004386 @default.
- W2906770339 cites W1740391705 @default.
- W2906770339 cites W1896191564 @default.
- W2906770339 cites W1973236424 @default.
- W2906770339 cites W1981292890 @default.
- W2906770339 cites W1982365935 @default.
- W2906770339 cites W1985697147 @default.
- W2906770339 cites W1990010926 @default.
- W2906770339 cites W2002807243 @default.
- W2906770339 cites W2006081560 @default.
- W2906770339 cites W2009228936 @default.
- W2906770339 cites W2011588489 @default.
- W2906770339 cites W2014620003 @default.
- W2906770339 cites W2023654730 @default.
- W2906770339 cites W2037737090 @default.
- W2906770339 cites W2041275896 @default.
- W2906770339 cites W2045141817 @default.
- W2906770339 cites W2048914205 @default.
- W2906770339 cites W2049748793 @default.
- W2906770339 cites W2057251858 @default.
- W2906770339 cites W2058967017 @default.
- W2906770339 cites W2060199055 @default.
- W2906770339 cites W2062004123 @default.
- W2906770339 cites W2064982059 @default.
- W2906770339 cites W2068704618 @default.
- W2906770339 cites W2071893133 @default.
- W2906770339 cites W2072650441 @default.
- W2906770339 cites W2077237199 @default.
- W2906770339 cites W2081455233 @default.
- W2906770339 cites W2082088208 @default.
- W2906770339 cites W2092598076 @default.
- W2906770339 cites W2096014362 @default.
- W2906770339 cites W2100222523 @default.
- W2906770339 cites W2104545570 @default.
- W2906770339 cites W2105371598 @default.
- W2906770339 cites W2110892287 @default.
- W2906770339 cites W2126481507 @default.
- W2906770339 cites W2128694323 @default.
- W2906770339 cites W2134879989 @default.
- W2906770339 cites W2140396634 @default.
- W2906770339 cites W2142272081 @default.
- W2906770339 cites W2143653864 @default.
- W2906770339 cites W2147496191 @default.
- W2906770339 cites W2149346046 @default.
- W2906770339 cites W2159769454 @default.
- W2906770339 cites W2162649783 @default.
- W2906770339 cites W2170703236 @default.
- W2906770339 cites W2199203801 @default.
- W2906770339 cites W2200895684 @default.
- W2906770339 cites W2328215289 @default.
- W2906770339 cites W2415250978 @default.
- W2906770339 cites W2505850911 @default.
- W2906770339 cites W2513606032 @default.
- W2906770339 cites W2562436083 @default.
- W2906770339 cites W2609752905 @default.
- W2906770339 cites W2771132608 @default.
- W2906770339 cites W2792352422 @default.
- W2906770339 cites W2793923874 @default.
- W2906770339 cites W4233258168 @default.
- W2906770339 cites W657083373 @default.
- W2906770339 cites W744693956 @default.
- W2906770339 doi "https://doi.org/10.1016/j.jep.2018.12.032" @default.
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