FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2906833114> ?p ?o ?g. }

• W2906833114 endingPage "498" @default.
• W2906833114 startingPage "493" @default.
• W2906833114 abstract "Immunostaining methods have generally been used not only for biological studies but also for clinical diagnoses for decades. However, recently, for these methods, improved rapidity and simplicity have been required for relevant techniques in laboratory research and medical applications. To this end, we present here a novel approach for designing fluorescent molecular rotor probes, i.e., N3-modified thioflavin T (ThT) derivatives, which enabled specific detection of interesting protein targets with sensitive fluorescence turn-on. As an example, we synthesized N3-( d-desthiobiotinyl-PEGylated) thioflavin T (ThT-PD) and N3-(cortisolyl-PEGylated) thioflavin T (ThT-PC) that carried d-desthiobioin and cortisol, respectively, via PEG linkers. Compared to those of the probes without the targets, ThT-PD and ThT-PC exhibited around 27- and 8-fold fluorescence intensities, respectively, with the target streptavidin and anti-cortisol antibody in excess of saturation, enabling quantitative detection of the targets. Furthermore, we successfully demonstrated the feasibility of ligand-tethering N3-ThT derivatives by the rapid specific staining of glucocorticoid receptors in cells, which was completed within only several minutes using ThT-PC after cell fixation, whereas it took ∼24 h for immunostaining to capture the corresponding fluorescence images." @default.
• W2906833114 created "2019-01-11" @default.
• W2906833114 creator A5017667026 @default.
• W2906833114 creator A5033203894 @default.
• W2906833114 creator A5045126234 @default.
• W2906833114 creator A5045249269 @default.
• W2906833114 creator A5049338628 @default.
• W2906833114 creator A5061134485 @default.
• W2906833114 creator A5081605522 @default.
• W2906833114 creator A5084905847 @default.
• W2906833114 date "2018-12-29" @default.
• W2906833114 modified "2023-09-25" @default.
• W2906833114 title "High-Contrast Facile Imaging with Target-Directing Fluorescent Molecular Rotors, the N3-Modified Thioflavin T Derivatives" @default.
• W2906833114 cites W1797211023 @default.
• W2906833114 cites W1965740503 @default.
• W2906833114 cites W1975247582 @default.
• W2906833114 cites W1975368779 @default.
• W2906833114 cites W1977790675 @default.
• W2906833114 cites W1982941258 @default.
• W2906833114 cites W1985709097 @default.
• W2906833114 cites W2015766045 @default.
• W2906833114 cites W2020746727 @default.
• W2906833114 cites W2033571739 @default.
• W2906833114 cites W2048031945 @default.
• W2906833114 cites W2051654117 @default.
• W2906833114 cites W2054184600 @default.
• W2906833114 cites W2059633037 @default.
• W2906833114 cites W2065749797 @default.
• W2906833114 cites W2066444150 @default.
• W2906833114 cites W2066767743 @default.
• W2906833114 cites W2078752284 @default.
• W2906833114 cites W2080961843 @default.
• W2906833114 cites W2093188138 @default.
• W2906833114 cites W2096799975 @default.
• W2906833114 cites W2110410009 @default.
• W2906833114 cites W2129157800 @default.
• W2906833114 cites W2144535585 @default.
• W2906833114 cites W2165596976 @default.
• W2906833114 cites W2270133955 @default.
• W2906833114 cites W2316724571 @default.
• W2906833114 cites W2324445849 @default.
• W2906833114 cites W2331054285 @default.
• W2906833114 cites W2334844468 @default.
• W2906833114 cites W2340453220 @default.
• W2906833114 cites W2413819133 @default.
• W2906833114 cites W2473454973 @default.
• W2906833114 cites W2553380770 @default.
• W2906833114 cites W2742098164 @default.
• W2906833114 cites W2755063207 @default.
• W2906833114 cites W2765677744 @default.
• W2906833114 cites W2789430139 @default.
• W2906833114 cites W2797505659 @default.
• W2906833114 cites W2809151709 @default.
• W2906833114 doi "https://doi.org/10.1021/acs.biochem.8b01181" @default.
• W2906833114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30595015" @default.
• W2906833114 hasPublicationYear "2018" @default.
• W2906833114 type Work @default.
• W2906833114 sameAs 2906833114 @default.
• W2906833114 citedByCount "4" @default.
• W2906833114 countsByYear W29068331142020 @default.
• W2906833114 countsByYear W29068331142021 @default.
• W2906833114 crossrefType "journal-article" @default.
• W2906833114 hasAuthorship W2906833114A5017667026 @default.
• W2906833114 hasAuthorship W2906833114A5033203894 @default.
• W2906833114 hasAuthorship W2906833114A5045126234 @default.
• W2906833114 hasAuthorship W2906833114A5045249269 @default.
• W2906833114 hasAuthorship W2906833114A5049338628 @default.
• W2906833114 hasAuthorship W2906833114A5061134485 @default.
• W2906833114 hasAuthorship W2906833114A5081605522 @default.
• W2906833114 hasAuthorship W2906833114A5084905847 @default.
• W2906833114 hasConcept C121332964 @default.
• W2906833114 hasConcept C12554922 @default.
• W2906833114 hasConcept C142724271 @default.
• W2906833114 hasConcept C185592680 @default.
• W2906833114 hasConcept C204232928 @default.
• W2906833114 hasConcept C2778896982 @default.
• W2906833114 hasConcept C2779134260 @default.
• W2906833114 hasConcept C4224716 @default.
• W2906833114 hasConcept C502032728 @default.
• W2906833114 hasConcept C62520636 @default.
• W2906833114 hasConcept C71924100 @default.
• W2906833114 hasConcept C86803240 @default.
• W2906833114 hasConcept C91881484 @default.
• W2906833114 hasConceptScore W2906833114C121332964 @default.
• W2906833114 hasConceptScore W2906833114C12554922 @default.
• W2906833114 hasConceptScore W2906833114C142724271 @default.
• W2906833114 hasConceptScore W2906833114C185592680 @default.
• W2906833114 hasConceptScore W2906833114C204232928 @default.
• W2906833114 hasConceptScore W2906833114C2778896982 @default.
• W2906833114 hasConceptScore W2906833114C2779134260 @default.
• W2906833114 hasConceptScore W2906833114C4224716 @default.
• W2906833114 hasConceptScore W2906833114C502032728 @default.
• W2906833114 hasConceptScore W2906833114C62520636 @default.
• W2906833114 hasConceptScore W2906833114C71924100 @default.
• W2906833114 hasConceptScore W2906833114C86803240 @default.
• W2906833114 hasConceptScore W2906833114C91881484 @default.
• W2906833114 hasFunder F4320311405 @default.
• W2906833114 hasFunder F4320334764 @default.

• next