FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2906993698> ?p ?o ?g. }

• W2906993698 endingPage "e0210297" @default.
• W2906993698 startingPage "e0210297" @default.
• W2906993698 abstract "Background Canine hemangiosarcoma (cHSA) is a highly metastatic mesenchymal cancer that disseminates by hematogenous and direct implantation routes. Therapies for cHSA are generally ineffective, in part due to advanced clinical disease stage at the time of diagnosis. The validation of conventional molecular methods for detecting novel biomarkers preferentially expressed by cHSA could lead to more timely diagnosis, earlier therapeutic interventions, and improved outcomes. In humans, prostate-specific membrane antigen (PSMA) is a transmembrane protein overexpressed by prostate carcinoma and tumor-associated endothelium of various solid cancer histologies. Importantly, the preferential overexpression of PSMA by certain cancers has been leveraged for the development of diagnostic molecular imaging reagents and targeted therapeutics. Recently, PSMA has been qualitatively demonstrated to be expressed in cHSA cell lines, however, quantitative PSMA expressions and the potential utility of PSMA transcript identification in biologic fluids to support the presence of microscopic cHSA burden has not been reported. Therefore, this study sought to characterize the differential quantitative expressions of PSMA between cHSA and non-malignant tissues, and to determine the potential diagnostic utility of PCR-generated PSMA amplicons as a surrogate of rare cHSA cells dwelling within peritoneal and pericardial cavities.Methods Quantitative gene and protein expressions for PSMA were compared between one normal endothelial and six cHSA cell lines by RT-PCR, western blot analysis, and fluorescent microscopy. Additionally, gene and protein expressions of PSMA in normal canine tissues were characterized. Graded expressions of PSMA were determined in spontaneously-arising cHSA tumor samples and the feasibility of qualitative PCR as a molecular diagnostic to detect PSMA transcripts in whole blood from healthy dogs and hemorrhagic effusions from cHSA-bearing dogs were evaluated.Results PSMA gene and protein expressions were elevated (up to 6-fold) in cHSA cells compared with non-malignant endothelium. By immunohistochemistry, protein expressions of PSMA were detectable in all cHSA tissue samples evaluated. As predicted by human protein atlas data, PSMA’s expression was comparably identified at substantial levels in select normal canine tissues including kidney, liver, and intestine. In young healthy pet dogs, PSMA amplicons could not be identified in circulating whole blood yet were detectable in hemorrhagic effusions collected from pet dogs with confirmed cHSA or PSMA-expressing cancer.Conclusions PSMA is quantitatively overexpressed in cHSA compared to normal endothelium, but its protein expression is not restricted to only cHSA tumor tissues, as specific visceral organs also substantively express PSMA. Optimized qualitative PCR methods failed to amplify PSMA amplicons sufficiently for visible detection in circulating whole blood derived from healthy young dogs, yet PSMA transcripts were readily identifiable in hemorrhagic effusions collected from pet dogs with histologically confirmed cHSA or PSMA-expressing cancer. While preliminary, findings derived from a limited cohort of normal and diseased pet dogs provocatively raise the potential value of PSMA amplicon detection as an ancillary molecular diagnostic test for supporting the presence of microscopic cHSA disease burden within hemorrhagic body cavity effusions." @default.
• W2906993698 created "2019-01-11" @default.
• W2906993698 creator A5012219010 @default.
• W2906993698 creator A5030545546 @default.
• W2906993698 creator A5050977078 @default.
• W2906993698 creator A5051370010 @default.
• W2906993698 creator A5069840927 @default.
• W2906993698 creator A5070343414 @default.
• W2906993698 creator A5080473106 @default.
• W2906993698 date "2019-01-02" @default.
• W2906993698 modified "2023-10-15" @default.
• W2906993698 title "Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions" @default.
• W2906993698 cites W1502093439 @default.
• W2906993698 cites W1588218207 @default.
• W2906993698 cites W1814183605 @default.
• W2906993698 cites W1963528534 @default.
• W2906993698 cites W1970720658 @default.
• W2906993698 cites W1971048935 @default.
• W2906993698 cites W1972922858 @default.
• W2906993698 cites W1979804110 @default.
• W2906993698 cites W2000206516 @default.
• W2906993698 cites W2005156700 @default.
• W2906993698 cites W2007747581 @default.
• W2906993698 cites W2010393075 @default.
• W2906993698 cites W2016610881 @default.
• W2906993698 cites W2016668321 @default.
• W2906993698 cites W2024811115 @default.
• W2906993698 cites W2026701007 @default.
• W2906993698 cites W2028821121 @default.
• W2906993698 cites W2060805145 @default.
• W2906993698 cites W2072451168 @default.
• W2906993698 cites W2074382993 @default.
• W2906993698 cites W2085217958 @default.
• W2906993698 cites W2093777757 @default.
• W2906993698 cites W2094484532 @default.
• W2906993698 cites W2101789570 @default.
• W2906993698 cites W2111791000 @default.
• W2906993698 cites W2114785296 @default.
• W2906993698 cites W2116672406 @default.
• W2906993698 cites W2116884624 @default.
• W2906993698 cites W2118292546 @default.
• W2906993698 cites W2119732961 @default.
• W2906993698 cites W2120567276 @default.
• W2906993698 cites W2134166614 @default.
• W2906993698 cites W2138610273 @default.
• W2906993698 cites W2146513847 @default.
• W2906993698 cites W2152869658 @default.
• W2906993698 cites W2158629604 @default.
• W2906993698 cites W2162407226 @default.
• W2906993698 cites W2163759862 @default.
• W2906993698 cites W2165085849 @default.
• W2906993698 cites W2198966903 @default.
• W2906993698 cites W2312684437 @default.
• W2906993698 cites W2407516299 @default.
• W2906993698 cites W2409065165 @default.
• W2906993698 cites W2513308461 @default.
• W2906993698 cites W2524354109 @default.
• W2906993698 cites W2529236338 @default.
• W2906993698 cites W2599298608 @default.
• W2906993698 cites W2607235507 @default.
• W2906993698 cites W2612027160 @default.
• W2906993698 cites W2750533313 @default.
• W2906993698 cites W2776393992 @default.
• W2906993698 cites W2799375096 @default.
• W2906993698 cites W2808713439 @default.
• W2906993698 cites W2885666779 @default.
• W2906993698 cites W335587689 @default.
• W2906993698 cites W4255434939 @default.
• W2906993698 cites W4296926832 @default.
• W2906993698 cites W569838262 @default.
• W2906993698 cites W2163702338 @default.
• W2906993698 doi "https://doi.org/10.1371/journal.pone.0210297" @default.
• W2906993698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6314605" @default.
• W2906993698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30601866" @default.
• W2906993698 hasPublicationYear "2019" @default.
• W2906993698 type Work @default.
• W2906993698 sameAs 2906993698 @default.
• W2906993698 citedByCount "6" @default.
• W2906993698 countsByYear W29069936982020 @default.
• W2906993698 countsByYear W29069936982021 @default.
• W2906993698 countsByYear W29069936982022 @default.
• W2906993698 crossrefType "journal-article" @default.
• W2906993698 hasAuthorship W2906993698A5012219010 @default.
• W2906993698 hasAuthorship W2906993698A5030545546 @default.
• W2906993698 hasAuthorship W2906993698A5050977078 @default.
• W2906993698 hasAuthorship W2906993698A5051370010 @default.
• W2906993698 hasAuthorship W2906993698A5069840927 @default.
• W2906993698 hasAuthorship W2906993698A5070343414 @default.
• W2906993698 hasAuthorship W2906993698A5080473106 @default.
• W2906993698 hasBestOaLocation W29069936981 @default.
• W2906993698 hasConcept C121608353 @default.
• W2906993698 hasConcept C126322002 @default.
• W2906993698 hasConcept C142724271 @default.
• W2906993698 hasConcept C147483822 @default.
• W2906993698 hasConcept C203014093 @default.
• W2906993698 hasConcept C20417620 @default.
• W2906993698 hasConcept C2776235491 @default.
• W2906993698 hasConcept C2779134260 @default.

• next