Matches in SemOpenAlex for { <https://semopenalex.org/work/W2907584513> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2907584513 endingPage "e372" @default.
- W2907584513 startingPage "e365" @default.
- W2907584513 abstract "In this study, we focused on the regulatory roles of miR-494 in the pathogenesis of intervertebral disk degeneration (IDD) and the related mechanism. First, rat IDD models were established, and the expression levels of miR-494 in IDDs of the rats were examined. Next, human nucleus pulposus (NP) cells were cultured and transfected with miR-494 mimics and inhibitors, and the roles of miR-494 on the proliferation and apoptosis of cells were determined using MTT cell proliferation assay and flow cytometry methods. Furthermore, the targeting relationship between miR-494 and neuro-oncological ventral antigen 1 (NOVA1) was examined by dual luciferase reporter assay. Finally, the expression of NOVA1, Caspase-3, Bcl-2–associated X protein (BAX), and B-cell lymphoma-2 (BCL-2) was examined using real-time quantitative polymerase chain reaction and western blot methods. The results demonstrated that the expression of miR-494 was significantly upregulated in IDD rats. Moreover, transfection of miR-494 inhibitors induced a significant increase in the proliferation and marked decrease in the apoptosis of the degenerated human NP cells. Transfection of miR-494 mimics has shown the opposite effects. Furthermore, NOVA1 has been confirmed as a target of miR-494, and the expressions of NOVA1 were significantly downregulated in IDD rats. In addition, transfection of miR-494 inhibitors significantly decreased the expression of Caspase-3 and BAX and markedly increased the expression of NOVA1 and BCL-2. Transfection of miR-494 mimics has shown the opposite effects. miR-494 was upregulated in IDD, and miR-494 might regulate the proliferation and apoptosis of NP cells through targeting NOVA1." @default.
- W2907584513 created "2019-01-11" @default.
- W2907584513 creator A5027475930 @default.
- W2907584513 creator A5071798264 @default.
- W2907584513 creator A5086664284 @default.
- W2907584513 date "2019-04-01" @default.
- W2907584513 modified "2023-09-23" @default.
- W2907584513 title "Roles of miR-494 in Intervertebral Disk Degeneration and the Related Mechanism" @default.
- W2907584513 cites W1755525288 @default.
- W2907584513 cites W2324864825 @default.
- W2907584513 cites W2514603268 @default.
- W2907584513 cites W2571845962 @default.
- W2907584513 cites W2591818121 @default.
- W2907584513 cites W2604279493 @default.
- W2907584513 cites W2605506485 @default.
- W2907584513 cites W2729026208 @default.
- W2907584513 cites W2744640215 @default.
- W2907584513 cites W2744746475 @default.
- W2907584513 cites W2768454775 @default.
- W2907584513 cites W2775040869 @default.
- W2907584513 cites W2775418754 @default.
- W2907584513 cites W2775826732 @default.
- W2907584513 cites W2789977071 @default.
- W2907584513 cites W2790598717 @default.
- W2907584513 cites W2791659991 @default.
- W2907584513 cites W2891933403 @default.
- W2907584513 cites W4236721785 @default.
- W2907584513 doi "https://doi.org/10.1016/j.wneu.2018.12.098" @default.
- W2907584513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30599248" @default.
- W2907584513 hasPublicationYear "2019" @default.
- W2907584513 type Work @default.
- W2907584513 sameAs 2907584513 @default.
- W2907584513 citedByCount "3" @default.
- W2907584513 countsByYear W29075845132022 @default.
- W2907584513 countsByYear W29075845132023 @default.
- W2907584513 crossrefType "journal-article" @default.
- W2907584513 hasAuthorship W2907584513A5027475930 @default.
- W2907584513 hasAuthorship W2907584513A5071798264 @default.
- W2907584513 hasAuthorship W2907584513A5086664284 @default.
- W2907584513 hasConcept C104317684 @default.
- W2907584513 hasConcept C127561419 @default.
- W2907584513 hasConcept C153911025 @default.
- W2907584513 hasConcept C190283241 @default.
- W2907584513 hasConcept C203014093 @default.
- W2907584513 hasConcept C2776415932 @default.
- W2907584513 hasConcept C2780783641 @default.
- W2907584513 hasConcept C502942594 @default.
- W2907584513 hasConcept C54009773 @default.
- W2907584513 hasConcept C54355233 @default.
- W2907584513 hasConcept C553184892 @default.
- W2907584513 hasConcept C55493867 @default.
- W2907584513 hasConcept C62112901 @default.
- W2907584513 hasConcept C71924100 @default.
- W2907584513 hasConcept C81885089 @default.
- W2907584513 hasConcept C86803240 @default.
- W2907584513 hasConcept C95444343 @default.
- W2907584513 hasConceptScore W2907584513C104317684 @default.
- W2907584513 hasConceptScore W2907584513C127561419 @default.
- W2907584513 hasConceptScore W2907584513C153911025 @default.
- W2907584513 hasConceptScore W2907584513C190283241 @default.
- W2907584513 hasConceptScore W2907584513C203014093 @default.
- W2907584513 hasConceptScore W2907584513C2776415932 @default.
- W2907584513 hasConceptScore W2907584513C2780783641 @default.
- W2907584513 hasConceptScore W2907584513C502942594 @default.
- W2907584513 hasConceptScore W2907584513C54009773 @default.
- W2907584513 hasConceptScore W2907584513C54355233 @default.
- W2907584513 hasConceptScore W2907584513C553184892 @default.
- W2907584513 hasConceptScore W2907584513C55493867 @default.
- W2907584513 hasConceptScore W2907584513C62112901 @default.
- W2907584513 hasConceptScore W2907584513C71924100 @default.
- W2907584513 hasConceptScore W2907584513C81885089 @default.
- W2907584513 hasConceptScore W2907584513C86803240 @default.
- W2907584513 hasConceptScore W2907584513C95444343 @default.
- W2907584513 hasLocation W29075845131 @default.
- W2907584513 hasLocation W29075845132 @default.
- W2907584513 hasOpenAccess W2907584513 @default.
- W2907584513 hasPrimaryLocation W29075845131 @default.
- W2907584513 hasRelatedWork W1511425935 @default.
- W2907584513 hasRelatedWork W2345180005 @default.
- W2907584513 hasRelatedWork W2348852207 @default.
- W2907584513 hasRelatedWork W2354760635 @default.
- W2907584513 hasRelatedWork W2371917364 @default.
- W2907584513 hasRelatedWork W2385612501 @default.
- W2907584513 hasRelatedWork W2389688926 @default.
- W2907584513 hasRelatedWork W2390313530 @default.
- W2907584513 hasRelatedWork W2555524919 @default.
- W2907584513 hasRelatedWork W3032579496 @default.
- W2907584513 hasVolume "124" @default.
- W2907584513 isParatext "false" @default.
- W2907584513 isRetracted "false" @default.
- W2907584513 magId "2907584513" @default.
- W2907584513 workType "article" @default.