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• W290767906 abstract "Research Article1 June 1982free access Seven mammalian aminoacyl-tRNA synthetases co-purified as high molecular weight entities are associated within the same complex. M. Mirande M. Mirande Search for more papers by this author Y. Gache Y. Gache Search for more papers by this author D. Le Corre D. Le Corre Search for more papers by this author J.P. Waller J.P. Waller Search for more papers by this author M. Mirande M. Mirande Search for more papers by this author Y. Gache Y. Gache Search for more papers by this author D. Le Corre D. Le Corre Search for more papers by this author J.P. Waller J.P. Waller Search for more papers by this author Author Information M. Mirande, Y. Gache, D. Le Corre and J.P. Waller The EMBO Journal (1982)1:733-736https://doi.org/10.1002/j.1460-2075.1982.tb01238.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Seven aminoacyl-tRNA synthetases from sheep liver were co-purified as high mol. wt. entities to constant specific activities. The purified multienzyme preparation displayed an apparent mol. wt. of approximately 10(6) and was composed of 11 distinct polypeptides, as revealed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). To test the assumption that all of these components were physically associated within the same complex, the purified preparation was subjected to immunoprecipitation by antibodies raised against its lysyl- or methionyl-tRNA synthetase component. Depending on the limiting concentrations of the specific antibodies used, from 5 to 40% of the input protein was recovered in the immunoprecipitate. Its polypeptide composition, as revealed by SDS-PAGE, was indistinguishable from that of the original material. The immunoprecipitation reaction was highly specific, as attested by the observation that IgG from nonimmunized rabbit failed to precipitate any of the 11 polypeptides, even when used in 30-fold molar excess over input protein. We conclude that co-precipitation of all of these polypeptides by antibodies directed against a single component of the purified preparation is a consequence of their physical association within the same multienzyme complex. Previous ArticleNext Article Volume 1Issue 61 June 1982In this issue RelatedDetailsLoading ..." @default.
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• W290767906 title "Seven mammalian aminoacyl-tRNA synthetases co-purified as high molecular weight entities are associated within the same complex." @default.
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• W290767906 doi "https://doi.org/10.1002/j.1460-2075.1982.tb01238.x" @default.
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