Matches in SemOpenAlex for { <https://semopenalex.org/work/W2907971844> ?p ?o ?g. }
- W2907971844 endingPage "56" @default.
- W2907971844 startingPage "48" @default.
- W2907971844 abstract "Sarcosine oxidase (SOX) gene from Thermomicrobium roseum DSM 5159 (GeneBank: ACM06094.1) was inserted into a pMA5 plasmid, expressed in Bacillus subtilis WB600 in soluble form, and then was purified. The purified TrSOX revealed a single band at approximately 43 kDa on 10% reducing SDS-PAGE gel, and was further confirmed by linear trap quadropole mass spectrometry (LTQ-MS) analysis. Regarding CD analysis, the secondary structure of TrSOX was composed of 32.7% α-helix, 17.8% β-sheet, 20.6% β-turn, and 28.5% random coil. Nano-DSC analysis showed that the melting temperature (Tm) and the denaturation enthalpy (ΔH) of TrSOX were 100 ℃ and 552.8 kJ/mol, which indicated that the enzyme should be with outstanding thermo stability. Using N-methylglycine (sarcosine) as substrate, the N-methyl group was depleted and hydrogen peroxide was produced, the Km, kcat and kcat/Km of TrSOX were 61.2 ± 4.3 mmol/L, 3.2 ± 0.2 min−1 and 52.2 L·mol−1 min−1, respectively; additionally, TrSOX showed well adaptation and stability in aqueous/solvent biphasic mixture. Using N-methyltryptophan as substrate, the Km, kcat and kcat/Km of TrSOX were 136.5 ± 7.8 mmol/L, 0.27 ± 0.02 min−1 and 1.98 L·mol−1 min−1, respectively. Additionally, TrSOX showed catalytic activity against other N-methyl or N-ethyl compounds; and for most of the N-methyl-L substrates, the enzyme revealed high chiral selectivity (>100)." @default.
- W2907971844 created "2019-01-11" @default.
- W2907971844 creator A5019712754 @default.
- W2907971844 creator A5024802061 @default.
- W2907971844 creator A5047193639 @default.
- W2907971844 creator A5066599847 @default.
- W2907971844 creator A5080577523 @default.
- W2907971844 date "2019-02-01" @default.
- W2907971844 modified "2023-10-17" @default.
- W2907971844 title "Soluble expression of Thermomicrobium roseum sarcosine oxidase and characterization of N-demethylation activity" @default.
- W2907971844 cites W1475435051 @default.
- W2907971844 cites W1550488132 @default.
- W2907971844 cites W1896971617 @default.
- W2907971844 cites W1954584384 @default.
- W2907971844 cites W1964065676 @default.
- W2907971844 cites W1967525201 @default.
- W2907971844 cites W1969336064 @default.
- W2907971844 cites W1974462461 @default.
- W2907971844 cites W1979639397 @default.
- W2907971844 cites W1980200511 @default.
- W2907971844 cites W1986764584 @default.
- W2907971844 cites W1990862957 @default.
- W2907971844 cites W1993775029 @default.
- W2907971844 cites W2003632243 @default.
- W2907971844 cites W2026742244 @default.
- W2907971844 cites W2037672521 @default.
- W2907971844 cites W2057642566 @default.
- W2907971844 cites W2066404668 @default.
- W2907971844 cites W2068610845 @default.
- W2907971844 cites W2068814309 @default.
- W2907971844 cites W2089461126 @default.
- W2907971844 cites W2093231216 @default.
- W2907971844 cites W2108102849 @default.
- W2907971844 cites W2108774921 @default.
- W2907971844 cites W2115889897 @default.
- W2907971844 cites W2131105522 @default.
- W2907971844 cites W2158082808 @default.
- W2907971844 cites W2173653744 @default.
- W2907971844 cites W2183185776 @default.
- W2907971844 cites W2317809844 @default.
- W2907971844 cites W2322949438 @default.
- W2907971844 cites W2404283831 @default.
- W2907971844 cites W2515376723 @default.
- W2907971844 cites W2525780049 @default.
- W2907971844 cites W2533015169 @default.
- W2907971844 cites W2623294445 @default.
- W2907971844 cites W2756497795 @default.
- W2907971844 cites W2791410839 @default.
- W2907971844 cites W2792947238 @default.
- W2907971844 cites W2802610943 @default.
- W2907971844 cites W2807373830 @default.
- W2907971844 cites W4256147669 @default.
- W2907971844 doi "https://doi.org/10.1016/j.mcat.2018.12.021" @default.
- W2907971844 hasPublicationYear "2019" @default.
- W2907971844 type Work @default.
- W2907971844 sameAs 2907971844 @default.
- W2907971844 citedByCount "6" @default.
- W2907971844 countsByYear W29079718442021 @default.
- W2907971844 countsByYear W29079718442022 @default.
- W2907971844 countsByYear W29079718442023 @default.
- W2907971844 crossrefType "journal-article" @default.
- W2907971844 hasAuthorship W2907971844A5019712754 @default.
- W2907971844 hasAuthorship W2907971844A5024802061 @default.
- W2907971844 hasAuthorship W2907971844A5047193639 @default.
- W2907971844 hasAuthorship W2907971844A5066599847 @default.
- W2907971844 hasAuthorship W2907971844A5080577523 @default.
- W2907971844 hasConcept C13965031 @default.
- W2907971844 hasConcept C161790260 @default.
- W2907971844 hasConcept C178790620 @default.
- W2907971844 hasConcept C181199279 @default.
- W2907971844 hasConcept C185592680 @default.
- W2907971844 hasConcept C18903297 @default.
- W2907971844 hasConcept C2777289219 @default.
- W2907971844 hasConcept C2777756961 @default.
- W2907971844 hasConcept C2779485144 @default.
- W2907971844 hasConcept C2779943223 @default.
- W2907971844 hasConcept C2781216036 @default.
- W2907971844 hasConcept C41183919 @default.
- W2907971844 hasConcept C43617362 @default.
- W2907971844 hasConcept C515207424 @default.
- W2907971844 hasConcept C55493867 @default.
- W2907971844 hasConcept C56856141 @default.
- W2907971844 hasConcept C71240020 @default.
- W2907971844 hasConcept C86803240 @default.
- W2907971844 hasConceptScore W2907971844C13965031 @default.
- W2907971844 hasConceptScore W2907971844C161790260 @default.
- W2907971844 hasConceptScore W2907971844C178790620 @default.
- W2907971844 hasConceptScore W2907971844C181199279 @default.
- W2907971844 hasConceptScore W2907971844C185592680 @default.
- W2907971844 hasConceptScore W2907971844C18903297 @default.
- W2907971844 hasConceptScore W2907971844C2777289219 @default.
- W2907971844 hasConceptScore W2907971844C2777756961 @default.
- W2907971844 hasConceptScore W2907971844C2779485144 @default.
- W2907971844 hasConceptScore W2907971844C2779943223 @default.
- W2907971844 hasConceptScore W2907971844C2781216036 @default.
- W2907971844 hasConceptScore W2907971844C41183919 @default.
- W2907971844 hasConceptScore W2907971844C43617362 @default.
- W2907971844 hasConceptScore W2907971844C515207424 @default.