Matches in SemOpenAlex for { <https://semopenalex.org/work/W2907992023> ?p ?o ?g. }
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- W2907992023 abstract "Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on capture of polyadenylated RNAs and cannot recover proteins that interact with nonadenylated RNAs, including long noncoding RNA, pre-mRNAs and bacterial RNAs. We present orthogonal organic phase separation (OOPS), which does not require molecular tagging or capture of polyadenylated RNA, and apply it to recover cross-linked protein-RNA and free protein, or protein-bound RNA and free RNA, in an unbiased way. We validated OOPS in HEK293, U2OS and MCF10A human cell lines, and show that 96% of proteins recovered were bound to RNA. We show that all long RNAs can be cross-linked to proteins, and recovered 1,838 RBPs, including 926 putative novel RBPs. OOPS is approximately 100-fold more efficient than existing methods and can enable analyses of dynamic RNA-protein interactions. We also characterize dynamic changes in RNA-protein interactions in mammalian cells following nocodazole arrest, and present a bacterial RNA-interactome for Escherichia coli. OOPS is compatible with downstream proteomics and RNA sequencing, and can be applied in any organism." @default.
- W2907992023 created "2019-01-11" @default.
- W2907992023 creator A5007256008 @default.
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- W2907992023 date "2019-01-03" @default.
- W2907992023 modified "2023-10-17" @default.
- W2907992023 title "Comprehensive identification of RNA–protein interactions in any organism using orthogonal organic phase separation (OOPS)" @default.
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