Matches in SemOpenAlex for { <https://semopenalex.org/work/W2908125437> ?p ?o ?g. }
- W2908125437 endingPage "583" @default.
- W2908125437 startingPage "572" @default.
- W2908125437 abstract "Endothelial cell (EC) dysfunction contributes to atherosclerosis, which is associated with arterial stiffening and fibronectin (FN) deposition, by ECs and smooth muscle cells (SMCs). The effect of stiffness on the EC/FN interaction and fibrillar adhesion formation has been poorly studied. An in vitro model was prepared that included FN-coated polydimethylsiloxane (PDMS) films with similar hydrophobicity and roughness but distinct Young's modulus values, mimicking healthy (1.0 MPa) and atherosclerotic (2.8 MPa) arteries. Human aortic abdominal endothelial cells (HAAECs) seeded on 1.0 MPa PDMS films spread over time and reached their maximum surface area faster than on 2.8 MPa PDMS films. In addition, HAAECs appeared to organize focal adhesion more rapidly on 1.0 MPa PDMS films, despite the similar cell binding domain accessibility to adsorbed FN. Interestingly, we also observed up to a ~5-fold increase in the percentage of HAAECs that had a well-developed fibrillar adhesion on 1.0 MPa compared to 2.8 MPa PDMS films as verified by integrin α5 subunits, tensin, and FN staining. This variation did not affect EC migration. These results suggest that there are favourable conditions for FN matrix assembly by ECs in early atherosclerosis rather than at advanced stages. Our in vitro model will therefore be helpful to understand the influence of bulk stiffness on cells involved in atherosclerosis." @default.
- W2908125437 created "2019-01-11" @default.
- W2908125437 creator A5003671277 @default.
- W2908125437 creator A5018756658 @default.
- W2908125437 creator A5022008825 @default.
- W2908125437 creator A5026323299 @default.
- W2908125437 creator A5054602562 @default.
- W2908125437 creator A5060505236 @default.
- W2908125437 creator A5062752837 @default.
- W2908125437 creator A5078656640 @default.
- W2908125437 date "2019-05-01" @default.
- W2908125437 modified "2023-10-18" @default.
- W2908125437 title "The effect of substrate bulk stiffness on focal and fibrillar adhesion formation in human abdominal aortic endothelial cells" @default.
- W2908125437 cites W1504989678 @default.
- W2908125437 cites W1789606850 @default.
- W2908125437 cites W1949072443 @default.
- W2908125437 cites W1962348298 @default.
- W2908125437 cites W1979712769 @default.
- W2908125437 cites W1981964901 @default.
- W2908125437 cites W2007684474 @default.
- W2908125437 cites W2012765742 @default.
- W2908125437 cites W2014220200 @default.
- W2908125437 cites W2018804148 @default.
- W2908125437 cites W2029271466 @default.
- W2908125437 cites W2041069947 @default.
- W2908125437 cites W2044895919 @default.
- W2908125437 cites W2053984681 @default.
- W2908125437 cites W2067282815 @default.
- W2908125437 cites W2084882688 @default.
- W2908125437 cites W2091422753 @default.
- W2908125437 cites W2093224327 @default.
- W2908125437 cites W2095994722 @default.
- W2908125437 cites W2096108882 @default.
- W2908125437 cites W2099423350 @default.
- W2908125437 cites W2100114559 @default.
- W2908125437 cites W2103674104 @default.
- W2908125437 cites W2106639911 @default.
- W2908125437 cites W2110389264 @default.
- W2908125437 cites W2110849081 @default.
- W2908125437 cites W2117362619 @default.
- W2908125437 cites W2118709967 @default.
- W2908125437 cites W2121961422 @default.
- W2908125437 cites W2127634832 @default.
- W2908125437 cites W2128777443 @default.
- W2908125437 cites W2139921980 @default.
- W2908125437 cites W2143311009 @default.
- W2908125437 cites W2146947344 @default.
- W2908125437 cites W2148038169 @default.
- W2908125437 cites W2152134291 @default.
- W2908125437 cites W2160152518 @default.
- W2908125437 cites W2165619954 @default.
- W2908125437 cites W2167909706 @default.
- W2908125437 cites W2252133910 @default.
- W2908125437 cites W2259256124 @default.
- W2908125437 cites W2275204317 @default.
- W2908125437 cites W2344056892 @default.
- W2908125437 cites W245208940 @default.
- W2908125437 cites W2472605685 @default.
- W2908125437 cites W2489907708 @default.
- W2908125437 cites W2516557409 @default.
- W2908125437 cites W2587625705 @default.
- W2908125437 cites W2589094127 @default.
- W2908125437 cites W2595645464 @default.
- W2908125437 cites W2599771473 @default.
- W2908125437 cites W2606706428 @default.
- W2908125437 cites W2617070021 @default.
- W2908125437 cites W2748137398 @default.
- W2908125437 cites W2760638133 @default.
- W2908125437 cites W2761716013 @default.
- W2908125437 cites W2782144965 @default.
- W2908125437 cites W2783409518 @default.
- W2908125437 cites W2889907755 @default.
- W2908125437 cites W573645376 @default.
- W2908125437 doi "https://doi.org/10.1016/j.msec.2018.12.130" @default.
- W2908125437 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30813060" @default.
- W2908125437 hasPublicationYear "2019" @default.
- W2908125437 type Work @default.
- W2908125437 sameAs 2908125437 @default.
- W2908125437 citedByCount "7" @default.
- W2908125437 countsByYear W29081254372019 @default.
- W2908125437 countsByYear W29081254372020 @default.
- W2908125437 countsByYear W29081254372022 @default.
- W2908125437 countsByYear W29081254372023 @default.
- W2908125437 crossrefType "journal-article" @default.
- W2908125437 hasAuthorship W2908125437A5003671277 @default.
- W2908125437 hasAuthorship W2908125437A5018756658 @default.
- W2908125437 hasAuthorship W2908125437A5022008825 @default.
- W2908125437 hasAuthorship W2908125437A5026323299 @default.
- W2908125437 hasAuthorship W2908125437A5054602562 @default.
- W2908125437 hasAuthorship W2908125437A5060505236 @default.
- W2908125437 hasAuthorship W2908125437A5062752837 @default.
- W2908125437 hasAuthorship W2908125437A5078656640 @default.
- W2908125437 hasConcept C12554922 @default.
- W2908125437 hasConcept C1491633281 @default.
- W2908125437 hasConcept C151166631 @default.
- W2908125437 hasConcept C159985019 @default.
- W2908125437 hasConcept C171250308 @default.
- W2908125437 hasConcept C185592680 @default.