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- W2908158667 abstract "以‘罗田甜柿'休眠芽茎尖诱导的愈伤组织为试材,对原生质体分离培养技术进行了研究.结果表明:(1)继代10 d的愈伤组织最适于分离原生质体;(2)优化的酶解体系为CPW+0.5%CellulaseOnoruka R-10+0.03%Pectinasefiom Aspergillus Niger+0.7 mol/L Mannitol+1%PVP～10;(3)原生质纯化方式以上浮法为好,100×g离心6 min;(4)琼脂糖念珠培养5～6 d观测到第1次分裂,多为不均等分裂,60～70d形成肉眼可见的小愈伤组织,愈伤组织增殖到5mm以上,分化成苗,转至生根培养基生根." @default.
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- W2908158667 date "2002-01-01" @default.
- W2908158667 modified "2023-09-26" @default.
- W2908158667 title "Studies on the Isolation, Culture of Protoplast and Plant Regerafion in Luofianfianshi Persimmon" @default.
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