FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2908780372> ?p ?o ?g. }

• W2908780372 endingPage "e0210970" @default.
• W2908780372 startingPage "e0210970" @default.
• W2908780372 abstract "The emergence of bacterial pathogens that are resistant to clinical antibiotics poses an increasing risk to human health. An important reservoir from which bacterial pathogens can acquire resistance is the human gut microbiota. However, thus far, a substantial fraction of the gut microbiota remains uncultivated and has been little-studied with respect to its resistance reservoir-function. Here, we aimed to isolate yet uncultivated resistant gut bacteria by a targeted approach. Therefore, faecal samples from 20 intensive care patients who had received the prophylactic antibiotic treatment selective digestive decontamination (SDD), i.e. tobramycin, polymyxin E, amphotericin B and cefotaxime, were inoculated anaerobically on porous aluminium oxide chips placed on top of poor and rich agar media, including media supplemented with the SDD antibiotics. Biomass growing on the chips was analysed by 16S rRNA gene amplicon sequencing, showing large inter-individual differences in bacterial cultivability, and enrichment of a range of taxonomically diverse operational taxonomic units (OTUs). Furthermore, growth of Ruminococcaceae (2 OTUs), Enterobacteriaceae (6 OTUs) and Lachnospiraceae (4 OTUs) was significantly inhibited by the SDD antibiotics. Strains belonging to 16 OTUs were candidates for cultivation to pure culture as they shared ≤95% sequence identity with the closest type strain and had a relative abundance of ≥2%. Six of these OTUs were detected on media containing SDD antibiotics, and as such were prime candidates to be studied regarding antibiotic resistance. One of these six OTUs was obtained in pure culture using targeted isolation. This novel strain was resistant to the antibiotics metrodinazole and imipenem. It was initially classified as member of the Ruminococcaceae, though later it was found to share 99% nucleotide identity with the recently published Sellimonas intestinalis BR72T. In conclusion, we show that high-throughput cultivation-based screening of microbial communities can guide targeted isolation of bacteria that serve as reservoirs of antibiotic resistance." @default.
• W2908780372 created "2019-01-25" @default.
• W2908780372 creator A5027532801 @default.
• W2908780372 creator A5040359679 @default.
• W2908780372 creator A5057074180 @default.
• W2908780372 creator A5068718584 @default.
• W2908780372 creator A5072770617 @default.
• W2908780372 date "2019-01-17" @default.
• W2908780372 modified "2023-09-27" @default.
• W2908780372 title "High throughput cultivation-based screening on porous aluminum oxide chips allows targeted isolation of antibiotic resistant human gut bacteria" @default.
• W2908780372 cites W1487425199 @default.
• W2908780372 cites W1741324201 @default.
• W2908780372 cites W1992298603 @default.
• W2908780372 cites W1992837433 @default.
• W2908780372 cites W1993645434 @default.
• W2908780372 cites W2012854889 @default.
• W2908780372 cites W2015761904 @default.
• W2908780372 cites W2027951608 @default.
• W2908780372 cites W2029036657 @default.
• W2908780372 cites W2033002674 @default.
• W2908780372 cites W2034285706 @default.
• W2908780372 cites W2037361537 @default.
• W2908780372 cites W2040382612 @default.
• W2908780372 cites W2042631505 @default.
• W2908780372 cites W2046501778 @default.
• W2908780372 cites W2047305814 @default.
• W2908780372 cites W2055043387 @default.
• W2908780372 cites W2057710367 @default.
• W2908780372 cites W2065088137 @default.
• W2908780372 cites W2067297267 @default.
• W2908780372 cites W2072706491 @default.
• W2908780372 cites W2072970694 @default.
• W2908780372 cites W2086606848 @default.
• W2908780372 cites W2089444561 @default.
• W2908780372 cites W2092901717 @default.
• W2908780372 cites W2099263747 @default.
• W2908780372 cites W2106524826 @default.
• W2908780372 cites W2115831350 @default.
• W2908780372 cites W2118838476 @default.
• W2908780372 cites W2122985424 @default.
• W2908780372 cites W2124372253 @default.
• W2908780372 cites W2128799206 @default.
• W2908780372 cites W2135801231 @default.
• W2908780372 cites W2144852012 @default.
• W2908780372 cites W2149290602 @default.
• W2908780372 cites W2151450070 @default.
• W2908780372 cites W2152609354 @default.
• W2908780372 cites W2154179335 @default.
• W2908780372 cites W2156978837 @default.
• W2908780372 cites W2164337734 @default.
• W2908780372 cites W2194052099 @default.
• W2908780372 cites W2258862982 @default.
• W2908780372 cites W2290520705 @default.
• W2908780372 cites W2326218659 @default.
• W2908780372 cites W2345813655 @default.
• W2908780372 cites W2393656244 @default.
• W2908780372 cites W2596882923 @default.
• W2908780372 cites W2619647536 @default.
• W2908780372 cites W2739852532 @default.
• W2908780372 doi "https://doi.org/10.1371/journal.pone.0210970" @default.
• W2908780372 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6336267" @default.
• W2908780372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30653573" @default.
• W2908780372 hasPublicationYear "2019" @default.
• W2908780372 type Work @default.
• W2908780372 sameAs 2908780372 @default.
• W2908780372 citedByCount "13" @default.
• W2908780372 countsByYear W29087803722019 @default.
• W2908780372 countsByYear W29087803722020 @default.
• W2908780372 countsByYear W29087803722021 @default.
• W2908780372 countsByYear W29087803722022 @default.
• W2908780372 countsByYear W29087803722023 @default.
• W2908780372 crossrefType "journal-article" @default.
• W2908780372 hasAuthorship W2908780372A5027532801 @default.
• W2908780372 hasAuthorship W2908780372A5040359679 @default.
• W2908780372 hasAuthorship W2908780372A5057074180 @default.
• W2908780372 hasAuthorship W2908780372A5068718584 @default.
• W2908780372 hasAuthorship W2908780372A5072770617 @default.
• W2908780372 hasBestOaLocation W29087803721 @default.
• W2908780372 hasConcept C203014093 @default.
• W2908780372 hasConcept C2777695942 @default.
• W2908780372 hasConcept C2779259085 @default.
• W2908780372 hasConcept C2779704485 @default.
• W2908780372 hasConcept C2780675486 @default.
• W2908780372 hasConcept C42062724 @default.
• W2908780372 hasConcept C501593827 @default.
• W2908780372 hasConcept C523546767 @default.
• W2908780372 hasConcept C539455810 @default.
• W2908780372 hasConcept C54355233 @default.
• W2908780372 hasConcept C86803240 @default.
• W2908780372 hasConcept C89423630 @default.
• W2908780372 hasConcept C94665300 @default.
• W2908780372 hasConceptScore W2908780372C203014093 @default.
• W2908780372 hasConceptScore W2908780372C2777695942 @default.
• W2908780372 hasConceptScore W2908780372C2779259085 @default.
• W2908780372 hasConceptScore W2908780372C2779704485 @default.
• W2908780372 hasConceptScore W2908780372C2780675486 @default.
• W2908780372 hasConceptScore W2908780372C42062724 @default.
• W2908780372 hasConceptScore W2908780372C501593827 @default.

• next