FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2909041805> ?p ?o ?g. }

• W2909041805 endingPage "734" @default.
• W2909041805 startingPage "715" @default.
• W2909041805 abstract "Campylobacter jejuni is a major gastrointestinal pathogen generally acquired via consumption of poorly prepared poultry. N-linked protein glycosylation encoded by the pgl gene cluster targets >80 membrane proteins and is required for both nonsymptomatic chicken colonization and full human virulence. Despite this, the biological functions of N-glycosylation remain unknown. We examined the effects of pgl gene deletion on the C. jejuni proteome using label-based liquid chromatography/tandem mass spectrometry (LC-MS/MS) and validation using data independent acquisition (DIA-SWATH-MS). We quantified 1359 proteins corresponding to ∼84% of the C. jejuni NCTC 11168 genome, and 1080 of these were validated by DIA-SWATH-MS. Deletion of the pglB oligosaccharyltransferase (ΔpglB) resulted in a significant change in abundance of 185 proteins, 137 of which were restored to their wild-type levels by reintroduction of pglB (Δaaz.batpglB::ΔpglB). Deletion of pglB was associated with significantly reduced abundances of pgl targets and increased stress-related proteins, including ClpB, GroEL, GroES, GrpE and DnaK. pglB mutants demonstrated reduced survival following temperature (4 °C and 46 °C) and osmotic (150 mm NaCl) shock and altered biofilm phenotypes compared with wild-type C. jejuni Targeted metabolomics established that pgl negative C. jejuni switched from aspartate (Asp) to proline (Pro) uptake and accumulated intracellular succinate related to proteome changes including elevated PutP/PutA (proline transport and utilization), and reduced DctA/DcuB (aspartate import and succinate export, respectively). ΔpglB chemotaxis to some substrates (Asp, glutamate, succinate and α-ketoglutarate) was reduced and associated with altered abundance of transducer-like (Tlp) proteins. Glycosylation negative C. jejuni were depleted of all respiration-associated proteins that allow the use of alternative electron acceptors under low oxygen. We demonstrate for the first time that N-glycosylation is required for a specific enzyme activity (Nap nitrate reductase) that is associated with reduced abundance of the NapAB glycoproteins. These data indicate a multifactorial role for N-glycosylation in C. jejuni physiology." @default.
• W2909041805 created "2019-01-25" @default.
• W2909041805 creator A5009846378 @default.
• W2909041805 creator A5028724289 @default.
• W2909041805 creator A5031503282 @default.
• W2909041805 creator A5033216655 @default.
• W2909041805 creator A5051257320 @default.
• W2909041805 creator A5053747472 @default.
• W2909041805 creator A5069339861 @default.
• W2909041805 creator A5074219458 @default.
• W2909041805 date "2019-04-01" @default.
• W2909041805 modified "2023-10-14" @default.
• W2909041805 title "Proteomics Reveals Multiple Phenotypes Associated with N-linked Glycosylation in Campylobacter jejuni" @default.
• W2909041805 cites W1542888060 @default.
• W2909041805 cites W1849301422 @default.
• W2909041805 cites W1964214446 @default.
• W2909041805 cites W1984646810 @default.
• W2909041805 cites W1985237517 @default.
• W2909041805 cites W1998453083 @default.
• W2909041805 cites W1999254509 @default.
• W2909041805 cites W2010927211 @default.
• W2909041805 cites W2018027663 @default.
• W2909041805 cites W2034400607 @default.
• W2909041805 cites W2034890237 @default.
• W2909041805 cites W2044608755 @default.
• W2909041805 cites W2049423615 @default.
• W2909041805 cites W2049565134 @default.
• W2909041805 cites W2063683785 @default.
• W2909041805 cites W2063731311 @default.
• W2909041805 cites W2065609478 @default.
• W2909041805 cites W2078703243 @default.
• W2909041805 cites W2088221752 @default.
• W2909041805 cites W2089058910 @default.
• W2909041805 cites W2089293580 @default.
• W2909041805 cites W2092995073 @default.
• W2909041805 cites W2100394298 @default.
• W2909041805 cites W2103740237 @default.
• W2909041805 cites W2104745339 @default.
• W2909041805 cites W2105118505 @default.
• W2909041805 cites W2116817862 @default.
• W2909041805 cites W2119492451 @default.
• W2909041805 cites W2120398179 @default.
• W2909041805 cites W2120904752 @default.
• W2909041805 cites W2127347515 @default.
• W2909041805 cites W2128981277 @default.
• W2909041805 cites W2133282365 @default.
• W2909041805 cites W2133676159 @default.
• W2909041805 cites W2135878201 @default.
• W2909041805 cites W2135973921 @default.
• W2909041805 cites W2141445066 @default.
• W2909041805 cites W2141898595 @default.
• W2909041805 cites W2142250977 @default.
• W2909041805 cites W2142342668 @default.
• W2909041805 cites W2144200574 @default.
• W2909041805 cites W2144611694 @default.
• W2909041805 cites W2150764734 @default.
• W2909041805 cites W2151144231 @default.
• W2909041805 cites W2151355155 @default.
• W2909041805 cites W2151582316 @default.
• W2909041805 cites W2152495803 @default.
• W2909041805 cites W2154592057 @default.
• W2909041805 cites W2155053954 @default.
• W2909041805 cites W2160197593 @default.
• W2909041805 cites W2161025101 @default.
• W2909041805 cites W2161171034 @default.
• W2909041805 cites W2165463709 @default.
• W2909041805 cites W2165851026 @default.
• W2909041805 cites W2165884082 @default.
• W2909041805 cites W2166211924 @default.
• W2909041805 cites W2168066825 @default.
• W2909041805 cites W2298081320 @default.
• W2909041805 cites W2328636580 @default.
• W2909041805 cites W2346707626 @default.
• W2909041805 cites W2400401435 @default.
• W2909041805 cites W2511075865 @default.
• W2909041805 cites W2572875517 @default.
• W2909041805 cites W2586315409 @default.
• W2909041805 cites W2587562821 @default.
• W2909041805 cites W2783262056 @default.
• W2909041805 cites W2803664483 @default.
• W2909041805 cites W2804977237 @default.
• W2909041805 cites W2808946469 @default.
• W2909041805 doi "https://doi.org/10.1074/mcp.ra118.001199" @default.
• W2909041805 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6442361" @default.
• W2909041805 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33451653" @default.
• W2909041805 hasPublicationYear "2019" @default.
• W2909041805 type Work @default.
• W2909041805 sameAs 2909041805 @default.
• W2909041805 citedByCount "58" @default.
• W2909041805 countsByYear W29090418052019 @default.
• W2909041805 countsByYear W29090418052020 @default.
• W2909041805 countsByYear W29090418052021 @default.
• W2909041805 countsByYear W29090418052022 @default.
• W2909041805 countsByYear W29090418052023 @default.
• W2909041805 crossrefType "journal-article" @default.
• W2909041805 hasAuthorship W2909041805A5009846378 @default.
• W2909041805 hasAuthorship W2909041805A5028724289 @default.
• W2909041805 hasAuthorship W2909041805A5031503282 @default.

• next