FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2909304876> ?p ?o ?g. }

• W2909304876 endingPage "e0210889" @default.
• W2909304876 startingPage "e0210889" @default.
• W2909304876 abstract "Histone modifications play a key role in the epigenetic regulation of gene transcription in cancer cells. Histone acetylations are regulated by two classes of enzymes, histone acetyltransferases (HATs) and histone deacetylases (HDACs). HDACs are increased in ovarian carcinomas and they are involved in carcinogenesis and resistance to chemotherapeutic agents. In our study we investigated anticancer effect of HDAC inhibitor sodium butyrate (NaBu) on cisplatin-sensitive and cisplatin-resistant ovarian cell lines A2780 and A2780cis. A2780 and A2780cis were treated with NaBu alone or in combination with cisplatin (CP). NaBu inhibited the growth of both cell lines and enhanced cytotoxic effect of CP. Exposure to NaBu for 24 h induced cell cycle arrest. The expressions of EMT-related genes and proteins were further investigated by qPCR and western blot analysis. Loss of E-cadherin has been shown to be crucial in ovarian cancer development. We found that NaBu dramatically induce expression of E-cadherin gene (CDH1) and protein levels in A2780 and A2780cis. We investigated correlation between transcription and methylation of CDH1gene. Methylation level analysis in 32 CpG sites in CDH1 gene (promoter/exon1 regions) was performed using bisulfite NGS (Next Generation Sequencing). We found that cisplatin-resistant cell line A2780cis cells differ from their cisplatin-sensitive counterparts in the CDH1 methylation. Methylation in A2780cis cells is elevated compared to A2780. However, NaBu-induced expression of CDH1 was not accompanied by CDH1 demethylation. NaBu treatment induced changes in expression of EMT-related genes and proteins. Interestingly E-cadherin zinc finger transcriptional repressor SNAIL1 was upregulated in both cell lines. Mesenchymal marker vimentin was downregulated. Matrix metalloproteases (MMPs) are necessary for pericellular proteolysis and facilitate migration and invasion of tumour cells. NaBu induced mRNA expression of MMPs, mild changes in activities of gelatinases MMP2 and MMP9 were detected. Our data demonstrate that NaBu sensitizes cisplatin-resistant ovarian cancer cells, re-established E-cadherin expression, but it was not able to reverse the EMT phenotype completely." @default.
• W2909304876 created "2019-01-25" @default.
• W2909304876 creator A5005255370 @default.
• W2909304876 creator A5022514220 @default.
• W2909304876 creator A5057865458 @default.
• W2909304876 creator A5079207718 @default.
• W2909304876 creator A5082282649 @default.
• W2909304876 creator A5082608608 @default.
• W2909304876 creator A5087493118 @default.
• W2909304876 creator A5090547366 @default.
• W2909304876 date "2019-01-17" @default.
• W2909304876 modified "2023-10-16" @default.
• W2909304876 title "The effect of sodium butyrate and cisplatin on expression of EMT markers" @default.
• W2909304876 cites W1185736310 @default.
• W2909304876 cites W13212225 @default.
• W2909304876 cites W1689351220 @default.
• W2909304876 cites W1875101685 @default.
• W2909304876 cites W1964847767 @default.
• W2909304876 cites W1970918455 @default.
• W2909304876 cites W1976731741 @default.
• W2909304876 cites W1977519421 @default.
• W2909304876 cites W1980561748 @default.
• W2909304876 cites W1991615588 @default.
• W2909304876 cites W1995417493 @default.
• W2909304876 cites W2013439755 @default.
• W2909304876 cites W2018590883 @default.
• W2909304876 cites W2037583144 @default.
• W2909304876 cites W2042083290 @default.
• W2909304876 cites W2058073053 @default.
• W2909304876 cites W2061396130 @default.
• W2909304876 cites W2070058781 @default.
• W2909304876 cites W2072003502 @default.
• W2909304876 cites W2072749701 @default.
• W2909304876 cites W2078945633 @default.
• W2909304876 cites W2080972062 @default.
• W2909304876 cites W2095382041 @default.
• W2909304876 cites W2096906921 @default.
• W2909304876 cites W2119108353 @default.
• W2909304876 cites W2121624265 @default.
• W2909304876 cites W2125970092 @default.
• W2909304876 cites W2126747397 @default.
• W2909304876 cites W2131099997 @default.
• W2909304876 cites W2131174392 @default.
• W2909304876 cites W2131250428 @default.
• W2909304876 cites W2132197309 @default.
• W2909304876 cites W2144481439 @default.
• W2909304876 cites W2144551784 @default.
• W2909304876 cites W2150416393 @default.
• W2909304876 cites W2152625770 @default.
• W2909304876 cites W2153104803 @default.
• W2909304876 cites W2161015001 @default.
• W2909304876 cites W2165901622 @default.
• W2909304876 cites W2171552768 @default.
• W2909304876 cites W2181788738 @default.
• W2909304876 cites W2541689020 @default.
• W2909304876 cites W2583446434 @default.
• W2909304876 cites W2587548185 @default.
• W2909304876 cites W2594404528 @default.
• W2909304876 doi "https://doi.org/10.1371/journal.pone.0210889" @default.
• W2909304876 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6336326" @default.
• W2909304876 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30653577" @default.
• W2909304876 hasPublicationYear "2019" @default.
• W2909304876 type Work @default.
• W2909304876 sameAs 2909304876 @default.
• W2909304876 citedByCount "28" @default.
• W2909304876 countsByYear W29093048762019 @default.
• W2909304876 countsByYear W29093048762020 @default.
• W2909304876 countsByYear W29093048762021 @default.
• W2909304876 countsByYear W29093048762022 @default.
• W2909304876 countsByYear W29093048762023 @default.
• W2909304876 crossrefType "journal-article" @default.
• W2909304876 hasAuthorship W2909304876A5005255370 @default.
• W2909304876 hasAuthorship W2909304876A5022514220 @default.
• W2909304876 hasAuthorship W2909304876A5057865458 @default.
• W2909304876 hasAuthorship W2909304876A5079207718 @default.
• W2909304876 hasAuthorship W2909304876A5082282649 @default.
• W2909304876 hasAuthorship W2909304876A5082608608 @default.
• W2909304876 hasAuthorship W2909304876A5087493118 @default.
• W2909304876 hasAuthorship W2909304876A5090547366 @default.
• W2909304876 hasBestOaLocation W29093048761 @default.
• W2909304876 hasConcept C104317684 @default.
• W2909304876 hasConcept C1491633281 @default.
• W2909304876 hasConcept C149402561 @default.
• W2909304876 hasConcept C150194340 @default.
• W2909304876 hasConcept C153911025 @default.
• W2909304876 hasConcept C185592680 @default.
• W2909304876 hasConcept C189235521 @default.
• W2909304876 hasConcept C190727270 @default.
• W2909304876 hasConcept C2776391639 @default.
• W2909304876 hasConcept C2776694085 @default.
• W2909304876 hasConcept C2777865847 @default.
• W2909304876 hasConcept C2778239845 @default.
• W2909304876 hasConcept C2778305200 @default.
• W2909304876 hasConcept C2778942008 @default.
• W2909304876 hasConcept C33288867 @default.
• W2909304876 hasConcept C41091548 @default.
• W2909304876 hasConcept C502942594 @default.
• W2909304876 hasConcept C54355233 @default.

• next