Matches in SemOpenAlex for { <https://semopenalex.org/work/W2909496509> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2909496509 endingPage "892" @default.
- W2909496509 startingPage "883" @default.
- W2909496509 abstract "Traditional enzyme-based methods for measurement of fructan were designed to measure just inulin and branched-type (agave) fructans. The enzymes employed, namely exo-inulinase and endo-inulinase, give incompletely hydrolysis of levan. Levan hydrolysis requires a third enzyme, endo-levanase. This paper describes a method and commercial test kit (Megazyme Fructan Assay Kit) for the determination of all types of fructan (inulin, levan, and branched) in a variety of animal feeds and pet foods. The method has been validated in a single laboratory for analysis of pure inulin, agave fructan, levan, and a range of fructan containing samples. Quantification is based on complete hydrolysis of fructan to fructose and glucose by a mixture of exo-inulinase, endo-inulinase, and endo-levanase, followed by measurement of these sugars using the PAHBAH reducing sugar method which gives the same color response with fructose and glucose. Before hydrolysis of fructan, interfering sucrose and starch in the sample are specifically hydrolyzed and removed by borohydride reduction. The single-laboratory validation (SLV) outlined in this document was performed on commercially available inulin (Raftiline) and agave fructan (Frutafit®), levan purified from Timothy grass, two grass samples, a sample of legume hay, two animal feeds and two barley flours, one of which (Barley MAX®) was genetically enriched in fructan through plant breeding. Parameters examined during the validation included working range, target selectivity, recovery, LOD, LOQ, trueness (bias), precision (repeatability and intermediate precision), robustness, and stability. The method is robust, quick, and simple." @default.
- W2909496509 created "2019-01-25" @default.
- W2909496509 creator A5027173851 @default.
- W2909496509 creator A5028497042 @default.
- W2909496509 creator A5058532631 @default.
- W2909496509 creator A5080820790 @default.
- W2909496509 creator A5086015781 @default.
- W2909496509 date "2019-05-01" @default.
- W2909496509 modified "2023-10-18" @default.
- W2909496509 title "Determination of Fructan (Inulin, FOS, Levan, and Branched Fructan) in Animal Food (Animal Feed, Pet Food, and Ingredients): Single-Laboratory Validation, First Action 2018.07" @default.
- W2909496509 doi "https://doi.org/10.5740/jaoacint.18-0330" @default.
- W2909496509 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30646972" @default.
- W2909496509 hasPublicationYear "2019" @default.
- W2909496509 type Work @default.
- W2909496509 sameAs 2909496509 @default.
- W2909496509 citedByCount "9" @default.
- W2909496509 countsByYear W29094965092020 @default.
- W2909496509 countsByYear W29094965092021 @default.
- W2909496509 countsByYear W29094965092022 @default.
- W2909496509 countsByYear W29094965092023 @default.
- W2909496509 crossrefType "journal-article" @default.
- W2909496509 hasAuthorship W2909496509A5027173851 @default.
- W2909496509 hasAuthorship W2909496509A5028497042 @default.
- W2909496509 hasAuthorship W2909496509A5058532631 @default.
- W2909496509 hasAuthorship W2909496509A5080820790 @default.
- W2909496509 hasAuthorship W2909496509A5086015781 @default.
- W2909496509 hasConcept C185592680 @default.
- W2909496509 hasConcept C2775884923 @default.
- W2909496509 hasConcept C2775980262 @default.
- W2909496509 hasConcept C2776970464 @default.
- W2909496509 hasConcept C2777108408 @default.
- W2909496509 hasConcept C2778456524 @default.
- W2909496509 hasConcept C2778597767 @default.
- W2909496509 hasConcept C2779742124 @default.
- W2909496509 hasConcept C2780236525 @default.
- W2909496509 hasConcept C31903555 @default.
- W2909496509 hasConcept C43617362 @default.
- W2909496509 hasConcept C55493867 @default.
- W2909496509 hasConcept C59822182 @default.
- W2909496509 hasConcept C73583747 @default.
- W2909496509 hasConcept C86803240 @default.
- W2909496509 hasConcept C94412978 @default.
- W2909496509 hasConceptScore W2909496509C185592680 @default.
- W2909496509 hasConceptScore W2909496509C2775884923 @default.
- W2909496509 hasConceptScore W2909496509C2775980262 @default.
- W2909496509 hasConceptScore W2909496509C2776970464 @default.
- W2909496509 hasConceptScore W2909496509C2777108408 @default.
- W2909496509 hasConceptScore W2909496509C2778456524 @default.
- W2909496509 hasConceptScore W2909496509C2778597767 @default.
- W2909496509 hasConceptScore W2909496509C2779742124 @default.
- W2909496509 hasConceptScore W2909496509C2780236525 @default.
- W2909496509 hasConceptScore W2909496509C31903555 @default.
- W2909496509 hasConceptScore W2909496509C43617362 @default.
- W2909496509 hasConceptScore W2909496509C55493867 @default.
- W2909496509 hasConceptScore W2909496509C59822182 @default.
- W2909496509 hasConceptScore W2909496509C73583747 @default.
- W2909496509 hasConceptScore W2909496509C86803240 @default.
- W2909496509 hasConceptScore W2909496509C94412978 @default.
- W2909496509 hasIssue "3" @default.
- W2909496509 hasLocation W29094965091 @default.
- W2909496509 hasLocation W29094965092 @default.
- W2909496509 hasOpenAccess W2909496509 @default.
- W2909496509 hasPrimaryLocation W29094965091 @default.
- W2909496509 hasRelatedWork W1995164356 @default.
- W2909496509 hasRelatedWork W2023517823 @default.
- W2909496509 hasRelatedWork W2055452315 @default.
- W2909496509 hasRelatedWork W2114083025 @default.
- W2909496509 hasRelatedWork W2134457396 @default.
- W2909496509 hasRelatedWork W2792548172 @default.
- W2909496509 hasRelatedWork W3193821736 @default.
- W2909496509 hasRelatedWork W4252403995 @default.
- W2909496509 hasRelatedWork W4288066519 @default.
- W2909496509 hasRelatedWork W86344671 @default.
- W2909496509 hasVolume "102" @default.
- W2909496509 isParatext "false" @default.
- W2909496509 isRetracted "false" @default.
- W2909496509 magId "2909496509" @default.
- W2909496509 workType "article" @default.