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• W2910216015 abstract "Event Abstract Back to Event Comparison of axonal-conduction velocity in developing primary cells and human iPSC-derived neurons Marie Engelene J. Obien1, 2*, Giulio Zorzi1, Michele Fiscella1, 2, Noelle Leary1 and Andreas R. Hierlemann1 1 Abteilung Biosysteme Wissenschaft und Technik, ETH Zürich, Switzerland 2 MaxWell Biosystems, Switzerland Neurons communicate through action potentials propagating along axons. In developing cell cultures, axonal arbor outgrowth indicates the formation of synaptic connections between neurons, which form networks. As axons regulate the transfer of information, we hypothesize that axonal conduction characteristics, e.g., axonal action potential amplitude and propagation velocity, may be indicative of the maturation state of cells and the strength of interneuronal connections. Here, we investigated the axonal-conduction characteristics of individual neurons, which have been tracked across multiple days. Using a high-density microelectrode array (HD-MEA, MaxOne, MaxWell Biosystems, Switzerland), we performed electrical imaging of 2-dimensional cell culture samples and identified single neurons. The HD-MEAs were used to detect extracellular action potentials (EAPs) at sub-cellular resolution and to extract the shapes of axonal arbors by means of spike-triggered averaging (Bakkum et al., Nature Communications, 2013). We plated the following cell types on the HD-MEA dishes: (1) primary cortical cells and (2) hippocampal cells from Wistar rats (embryonic day 18); human-induced-pluripotent-stem-cell (h-iPSC)-derived dopaminergic neurons (iCell DopaNeurons, Cellular Dynamics International or CDI, USA) in (3) monoculture and (4) in co-culture with h-iPSC-derived astrocytes (iCell Astrocytes, CDI, USA); (5) h-iPSC-derived dopaminergic neurons modeling Parkinson’s disease (myCell A53T DopaNeurons, CDI, USA) in monoculture and (6) in co-culture with h-iPSC-derived astrocytes. Upon extraction of axonal morphologies through spike-triggered averaging, we computed the propagation velocity of the axonal action potentials per neuron. We observed that across multiple days, both, the spike amplitude and propagation velocity increased during development of healthy cells in culture. We compared axonal conduction features between primary cells and h-iPSC derived neurons in healthy and diseased state and found significant differences. In summary, HD-MEAs with sufficient SNRs provided a reliable platform for label-free and long-term investigation of axonal functions in h-iPSC neuronal cultures. Our results indicated that axonal conduction velocity may be a valuable functional parameter for disease modeling, drug discovery, and safety pharmacology. Acknowledgements Financial support through the H2020 ERC Advanced Grant 2015 - 694829 “neuroXscales” (Microtechnology and integrated microsystems to investigate neuronal networks across scales) and Project CTI-No. 25933.2 PFLS-LS (Multi-well electrophysiology platform for high-throughput cell-based assays) are acknowledged. References Bakkum, Douglas J., et al. Tracking axonal action potential propagation on a high-density microelectrode array across hundreds of sites. Nature communications 4 (2013): 2181. Keywords: Axons, h-iPSC, axonal conduction speed, cell culture, Multielectrode array (MEA), CMOS-MEAs, neuronal development Conference: MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays, Reutlingen, Germany, 4 Jul - 6 Jul, 2018. Presentation Type: Poster Presentation Topic: Stem cell-derived applications Citation: Obien MJ, Zorzi G, Fiscella M, Leary N and Hierlemann AR (2019). Comparison of axonal-conduction velocity in developing primary cells and human iPSC-derived neurons. Conference Abstract: MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays. doi: 10.3389/conf.fncel.2018.38.00095 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Mar 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Marie Engelene J Obien, Abteilung Biosysteme Wissenschaft und Technik, ETH Zürich, Zurich, 4058, Switzerland, marie.obien@mxwbio.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marie Engelene J Obien Giulio Zorzi Michele Fiscella Noelle Leary Andreas R Hierlemann Google Marie Engelene J Obien Giulio Zorzi Michele Fiscella Noelle Leary Andreas R Hierlemann Google Scholar Marie Engelene J Obien Giulio Zorzi Michele Fiscella Noelle Leary Andreas R Hierlemann PubMed Marie Engelene J Obien Giulio Zorzi Michele Fiscella Noelle Leary Andreas R Hierlemann Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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• W2910216015 title "Comparison of axonal-conduction velocity in developing primary cells and human iPSC-derived neurons" @default.
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