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- W2910257908 abstract "Abstract A new label‐free in situ monitoring system for the hybridization chain reaction (HCR) based on DNA minor‐groove‐binding fluorophores [Hoechst 33258 (Hoe) or quinone cyanine‐dithiazole (QCy‐DT)] has been developed. Use of two unmodified hairpin oligodeoxyribonucleotides containing incomplete double‐stranded AATT sequences enabled target‐dependent formation of probe binding sites—that is, AATT double strand—in the HCR product, together with fluorescence enhancement of minor‐groove‐binding fluorophores in situ. This system allows target DNA to be detected through the fluorescence enhancement of Hoe and QCy‐DT in real time and in situ. Further development of a label‐free, isothermal detection system might provide a cost‐effective and user‐friendly method for nucleic acid detection." @default.
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- W2910257908 date "2019-03-26" @default.
- W2910257908 modified "2023-09-23" @default.
- W2910257908 title "Label‐Free in Situ Monitoring of the DNA Hybridization Chain Reaction by Using Sequence‐Selective Minor‐Groove‐Binding Fluorophores" @default.
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- W2910257908 doi "https://doi.org/10.1002/cbic.201800738" @default.
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