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• W2910264531 abstract "Three-dimensional structured illumination microscopy (3D-SIM) is a wide-field super-resolution technique in fluorescent imaging that can double the resolution beyond its classical limit. We introduce, to the best of our knowledge, a new 3D reconstruction algorithm based on the Richardson–Lucy deconvolution. The 3D-SIM imaging principle and the reconstruction steps are demonstrated in detail. Microspheres and biological specimen are used to present the performance of this method. The background of the out-of-focus portion is effectively suppressed, and true optical sectioning and super-resolution can be achieved simultaneously. For the custom-built 3D-SIM and this reconstruction algorithm, the measured resolution was 99.5±5 nm laterally and 294±9 nm axially." @default.
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• W2910264531 date "2019-01-14" @default.
• W2910264531 modified "2023-10-01" @default.
• W2910264531 title "Super-resolution algorithm based on Richardson–Lucy deconvolution for three-dimensional structured illumination microscopy" @default.
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• W2910264531 doi "https://doi.org/10.1364/josaa.36.000173" @default.
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