FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2910473118> ?p ?o ?g. }

• W2910473118 endingPage "596" @default.
• W2910473118 startingPage "575" @default.
• W2910473118 abstract "Abstract Super-resolution fluorescence microscopy, also known as nanoscopy, has provided us with a glimpse of future impacts on cell biology. Far-field optical nanoscopy allows, for the first time, the study of sub-cellular nanoscale biological structures in living cells, which in the past was limited to electron microscopy (EM) (in fixed/dehydrated) cells or tissues. Nanoscopy has particular utility in the study of “fenestrations” – phospholipid transmembrane nanopores of 50–150 nm in diameter through liver sinusoidal endothelial cells (LSECs) that facilitate the passage of plasma, but (usually) not blood cells, to and from the surrounding hepatocytes. Previously, these fenestrations were only discernible with EM, but now they can be visualized in fixed and living cells using structured illumination microscopy (SIM) and in fixed cells using single molecule localization microscopy (SMLM) techniques such as direct stochastic optical reconstruction microscopy. Importantly, both methods use wet samples, avoiding dehydration artifacts. The use of nanoscopy can be extended to the in vitro study of fenestration dynamics, to address questions such as the following: are they actually dynamic structures, and how do they respond to endogenous and exogenous agents? A logical further extension of these methodologies to liver research (including the liver endothelium) will be their application to liver tissue sections from animal models with different pathological manifestations and ultimately to patient biopsies. This review will cover the current state of the art of the use of nanoscopy in the study of liver endothelium and the liver in general. Potential future applications in cell biology and the clinical implications will be discussed." @default.
• W2910473118 created "2019-01-25" @default.
• W2910473118 creator A5000126709 @default.
• W2910473118 creator A5009909151 @default.
• W2910473118 creator A5040780171 @default.
• W2910473118 creator A5049964953 @default.
• W2910473118 creator A5057144544 @default.
• W2910473118 creator A5061532003 @default.
• W2910473118 creator A5078514852 @default.
• W2910473118 creator A5079112852 @default.
• W2910473118 date "2018-02-23" @default.
• W2910473118 modified "2023-10-17" @default.
• W2910473118 title "New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations" @default.
• W2910473118 cites W1480262878 @default.
• W2910473118 cites W1553693482 @default.
• W2910473118 cites W1677311279 @default.
• W2910473118 cites W1781806439 @default.
• W2910473118 cites W1885888394 @default.
• W2910473118 cites W1964487917 @default.
• W2910473118 cites W1966426192 @default.
• W2910473118 cites W1978249718 @default.
• W2910473118 cites W1981916955 @default.
• W2910473118 cites W1982807939 @default.
• W2910473118 cites W1985280536 @default.
• W2910473118 cites W1987029683 @default.
• W2910473118 cites W1987227626 @default.
• W2910473118 cites W1992858116 @default.
• W2910473118 cites W1994304532 @default.
• W2910473118 cites W1995067743 @default.
• W2910473118 cites W1995413508 @default.
• W2910473118 cites W1996221028 @default.
• W2910473118 cites W1999085806 @default.
• W2910473118 cites W1999450541 @default.
• W2910473118 cites W2001022761 @default.
• W2910473118 cites W2001083377 @default.
• W2910473118 cites W2003659611 @default.
• W2910473118 cites W2007198833 @default.
• W2910473118 cites W2008711781 @default.
• W2910473118 cites W2011359102 @default.
• W2910473118 cites W2012826930 @default.
• W2910473118 cites W2015600303 @default.
• W2910473118 cites W2016189298 @default.
• W2910473118 cites W2018986985 @default.
• W2910473118 cites W2022158172 @default.
• W2910473118 cites W2025794520 @default.
• W2910473118 cites W2028883391 @default.
• W2910473118 cites W2033667053 @default.
• W2910473118 cites W2033788106 @default.
• W2910473118 cites W2035988096 @default.
• W2910473118 cites W2041302772 @default.
• W2910473118 cites W2042507145 @default.
• W2910473118 cites W2044882055 @default.
• W2910473118 cites W2045006795 @default.
• W2910473118 cites W2047190365 @default.
• W2910473118 cites W2051206690 @default.
• W2910473118 cites W2053697885 @default.
• W2910473118 cites W2054358220 @default.
• W2910473118 cites W2054455698 @default.
• W2910473118 cites W2055451169 @default.
• W2910473118 cites W2056963450 @default.
• W2910473118 cites W2058612331 @default.
• W2910473118 cites W2059472882 @default.
• W2910473118 cites W2061422980 @default.
• W2910473118 cites W2062552264 @default.
• W2910473118 cites W2064173118 @default.
• W2910473118 cites W2069757245 @default.
• W2910473118 cites W2072759932 @default.
• W2910473118 cites W2075030758 @default.
• W2910473118 cites W2075119852 @default.
• W2910473118 cites W2076115100 @default.
• W2910473118 cites W2076425962 @default.
• W2910473118 cites W2077746083 @default.
• W2910473118 cites W2080371259 @default.
• W2910473118 cites W2087850846 @default.
• W2910473118 cites W2088939076 @default.
• W2910473118 cites W2089725545 @default.
• W2910473118 cites W2091119704 @default.
• W2910473118 cites W2092006545 @default.
• W2910473118 cites W2100577920 @default.
• W2910473118 cites W2101336703 @default.
• W2910473118 cites W2101489505 @default.
• W2910473118 cites W2106316394 @default.
• W2910473118 cites W2106395637 @default.
• W2910473118 cites W2109962207 @default.
• W2910473118 cites W2114797146 @default.
• W2910473118 cites W2115737236 @default.
• W2910473118 cites W2116065010 @default.
• W2910473118 cites W2118583944 @default.
• W2910473118 cites W2120504118 @default.
• W2910473118 cites W2120707422 @default.
• W2910473118 cites W2121642161 @default.
• W2910473118 cites W2122179897 @default.
• W2910473118 cites W2123759854 @default.
• W2910473118 cites W2123888680 @default.
• W2910473118 cites W2125442511 @default.
• W2910473118 cites W2125505095 @default.
• W2910473118 cites W2130905710 @default.
• W2910473118 cites W2135613921 @default.

• next