Matches in SemOpenAlex for { <https://semopenalex.org/work/W2910656020> ?p ?o ?g. }
- W2910656020 endingPage "1400" @default.
- W2910656020 startingPage "1386" @default.
- W2910656020 abstract "Fluorescence, visible circular dichroism (CD), absorption, and resonance Raman spectroscopy techniques were combined to explore structural changes of ferricytochrome c upon its binding to cardiolipin-containing liposomes (20% 1,1',1,2'-tetraoleyolcardiolipin and 1,2-deoleyol-sn-glycero-3-phosphocholine) at acidic pH (6.5). According to the earlier work of Kawai [J. Biol. Chem.2005, 280, 34709-347171],cytochrome c binding at this pH is governed by interactions between the phosphate head groups of cardiolipin and amino acid side chains of the so-called L-site, which contains the charged residues K22, K25, K27, and potentially H26 and H33. We found that L-site binding causes a conformational transition that involves a change of the protein's ligation and spin state. In this paper, we report spectroscopic responses to an increasing number of cardiolipin-containing liposomes at pH 6.5 in the absence and presence of NaCl. The latter was found to mostly inhibit protein binding already with 50 mM concentration. The inhibition effect can be quantitatively reproduced by applying the electrostatic theory of Heimburg [Biophys. J.1995, 68, 536-546]. A comparison with corresponding spectroscopic response data obtained at pH 7.4 reveals major differences in that the latter indicates hydrophobic binding, followed by an electrostatically driven conformational change. Visible CD data suggest that structural changes in the heme pocket of liposome-bound ferricytochrome c resemble to some extent those in the denatured protein in urea at neutral and acidic pH. The measured noncoincidence between absorption and CD Soret band of cytochrome c in the presence of a large access of cardiolipin is caused by the electric field at the membrane surface. The very fact that its contribution to the internal electric field in the heme pocket is detectable by spectroscopic means suggests some penetration of the protein into membrane surface." @default.
- W2910656020 created "2019-01-25" @default.
- W2910656020 creator A5027210122 @default.
- W2910656020 creator A5071942997 @default.
- W2910656020 creator A5086321022 @default.
- W2910656020 creator A5089130125 @default.
- W2910656020 date "2019-01-16" @default.
- W2910656020 modified "2023-09-30" @default.
- W2910656020 title "pH-Induced Switch between Different Modes of Cytochrome <i>c</i> Binding to Cardiolipin-Containing Liposomes" @default.
- W2910656020 cites W1491243950 @default.
- W2910656020 cites W1514650462 @default.
- W2910656020 cites W1551289009 @default.
- W2910656020 cites W1945465158 @default.
- W2910656020 cites W1963920961 @default.
- W2910656020 cites W1968361213 @default.
- W2910656020 cites W1974243561 @default.
- W2910656020 cites W1976112620 @default.
- W2910656020 cites W1976350497 @default.
- W2910656020 cites W1983644819 @default.
- W2910656020 cites W1988336048 @default.
- W2910656020 cites W1990118523 @default.
- W2910656020 cites W1999690636 @default.
- W2910656020 cites W1999774021 @default.
- W2910656020 cites W2000634454 @default.
- W2910656020 cites W2001608530 @default.
- W2910656020 cites W2002437297 @default.
- W2910656020 cites W2002621746 @default.
- W2910656020 cites W2006935098 @default.
- W2910656020 cites W2008767221 @default.
- W2910656020 cites W2011222301 @default.
- W2910656020 cites W2011830892 @default.
- W2910656020 cites W2012553260 @default.
- W2910656020 cites W2013299248 @default.
- W2910656020 cites W2016812184 @default.
- W2910656020 cites W2032347469 @default.
- W2910656020 cites W2033895258 @default.
- W2910656020 cites W2034273954 @default.
- W2910656020 cites W2036826621 @default.
- W2910656020 cites W2037650101 @default.
- W2910656020 cites W2037736115 @default.
- W2910656020 cites W2043392247 @default.
- W2910656020 cites W2044848883 @default.
- W2910656020 cites W2046152827 @default.
- W2910656020 cites W2048649216 @default.
- W2910656020 cites W2049463380 @default.
- W2910656020 cites W2054739775 @default.
- W2910656020 cites W2055567342 @default.
- W2910656020 cites W2074444981 @default.
- W2910656020 cites W2075974661 @default.
- W2910656020 cites W2078074876 @default.
- W2910656020 cites W2079371335 @default.
- W2910656020 cites W2081197800 @default.
- W2910656020 cites W2083492588 @default.
- W2910656020 cites W2084998268 @default.
- W2910656020 cites W2086522192 @default.
- W2910656020 cites W2088790846 @default.
- W2910656020 cites W2091755047 @default.
- W2910656020 cites W2099261279 @default.
- W2910656020 cites W2105051996 @default.
- W2910656020 cites W2112501934 @default.
- W2910656020 cites W2121725552 @default.
- W2910656020 cites W2122028337 @default.
- W2910656020 cites W2165898505 @default.
- W2910656020 cites W2259844124 @default.
- W2910656020 cites W2288318116 @default.
- W2910656020 cites W2295199541 @default.
- W2910656020 cites W2317722512 @default.
- W2910656020 cites W2321994809 @default.
- W2910656020 cites W2339294105 @default.
- W2910656020 cites W2542851683 @default.
- W2910656020 cites W2549155751 @default.
- W2910656020 cites W2553658517 @default.
- W2910656020 cites W2589213448 @default.
- W2910656020 cites W2606855035 @default.
- W2910656020 cites W2748439441 @default.
- W2910656020 cites W2762968408 @default.
- W2910656020 cites W2789465514 @default.
- W2910656020 cites W2891053995 @default.
- W2910656020 cites W4211002055 @default.
- W2910656020 cites W4242586143 @default.
- W2910656020 doi "https://doi.org/10.1021/acsomega.8b02574" @default.
- W2910656020 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6647999" @default.
- W2910656020 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31459406" @default.
- W2910656020 hasPublicationYear "2019" @default.
- W2910656020 type Work @default.
- W2910656020 sameAs 2910656020 @default.
- W2910656020 citedByCount "17" @default.
- W2910656020 countsByYear W29106560202019 @default.
- W2910656020 countsByYear W29106560202020 @default.
- W2910656020 countsByYear W29106560202021 @default.
- W2910656020 countsByYear W29106560202022 @default.
- W2910656020 countsByYear W29106560202023 @default.
- W2910656020 crossrefType "journal-article" @default.
- W2910656020 hasAuthorship W2910656020A5027210122 @default.
- W2910656020 hasAuthorship W2910656020A5071942997 @default.
- W2910656020 hasAuthorship W2910656020A5086321022 @default.
- W2910656020 hasAuthorship W2910656020A5089130125 @default.
- W2910656020 hasBestOaLocation W29106560201 @default.