FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2910786482> ?p ?o ?g. }

• W2910786482 abstract "Abstract Pseudomonas aeruginosa frequently resides among ethanol-producing microbes, making its response to these microbially-produced concentrations of ethanol relevant to understanding its biology. Our ranscriptome analysis found that the genes involved in trehalose metabolism were induced by low concentrations of ethanol, and levels of intracellular trehalose increased significantly upon growth with ethanol. The increase in trehalose was dependent on the TreYZ pathway, but not other trehalose metabolic enzymes TreS or TreA. The sigma factor AlgU (AlgT), a homolog of RpoE in other species, was required for increased expression of the treZ gene and trehalose levels, but induction was not controlled by the well-characterized proteolysis of its antisigma factor MucA. Growth with ethanol led to increased SpoT-dependent (p)ppGpp accumulation, which stimulates AlgU-dependent transcription of treZ and other AlgU-regulated genes through DksA, a (p)ppGpp and RNA polymerase binding protein. Ethanol stimulation of trehalose also required acylhomoserine lactone (AHL)-mediated quorum sensing, as induction was not observed in a Δ lasR Δ rhlR strain. A network analysis using a model, eADAGE, built from publicly available P. aeruginosa transcriptome datasets (1) provided strong support for our model that treZ and co-regulated genes are controlled by both AlgU and AHL-mediated QS (QS). Consistent with (p)ppGpp and AHL-mediated quorum sensing regulation, ethanol, even when added at the time of culture inoculation, stimulated treZ transcript levels and trehalose production in cells from post-exponential phase cultures but not from exponential phase cultures. These data highlight the integration of growth and cell density cues in the P. aeruginosa transcriptional response to ethanol. Importance Pseudomonas aeruginosa is often found with bacteria and fungi that produce fermentation products including ethanol. At concentrations similar to those produced by environmental microbes, we found that ethanol stimulated expression of trehalose biosynthetic genes and cellular levels of trehalose, a disaccharide that protects against environmental stresses. The induction of trehalose by ethanol required the alternative sigma factor AlgU through DksA and SpoT-dependent (p)ppGpp. Trehalose accumulation also required AHL quorum sensing and only occurred in post-exponential phase cultures. This work highlights how cells integrate cell-density and growth cues in their responses to products made by other microbes and a reveals a new role for (p)ppGpp in the regulation of AlgU activity." @default.
• W2910786482 created "2019-01-25" @default.
• W2910786482 creator A5005067201 @default.
• W2910786482 creator A5006355040 @default.
• W2910786482 creator A5013463468 @default.
• W2910786482 creator A5034521980 @default.
• W2910786482 creator A5037962789 @default.
• W2910786482 creator A5045250449 @default.
• W2910786482 creator A5069687040 @default.
• W2910786482 date "2019-01-18" @default.
• W2910786482 modified "2023-09-24" @default.
• W2910786482 title "Ethanol stimulates trehalose production through a SpoT-DksA-AlgU dependent pathway in Pseudomonas aeruginosa" @default.
• W2910786482 cites W1492989453 @default.
• W2910786482 cites W1497023129 @default.
• W2910786482 cites W1509847467 @default.
• W2910786482 cites W1565401097 @default.
• W2910786482 cites W1569981240 @default.
• W2910786482 cites W1579175606 @default.
• W2910786482 cites W1587799736 @default.
• W2910786482 cites W1597428765 @default.
• W2910786482 cites W1616230851 @default.
• W2910786482 cites W1634834580 @default.
• W2910786482 cites W1674719483 @default.
• W2910786482 cites W1781531090 @default.
• W2910786482 cites W1827921954 @default.
• W2910786482 cites W1867307269 @default.
• W2910786482 cites W1889683180 @default.
• W2910786482 cites W1943327251 @default.
• W2910786482 cites W1963825954 @default.
• W2910786482 cites W1963949581 @default.
• W2910786482 cites W1972864313 @default.
• W2910786482 cites W1977938917 @default.
• W2910786482 cites W1985692996 @default.
• W2910786482 cites W1989074746 @default.
• W2910786482 cites W1993521681 @default.
• W2910786482 cites W1993765915 @default.
• W2910786482 cites W1996050193 @default.
• W2910786482 cites W1999046178 @default.
• W2910786482 cites W1999073796 @default.
• W2910786482 cites W2002545509 @default.
• W2910786482 cites W2013323516 @default.
• W2910786482 cites W2019176322 @default.
• W2910786482 cites W2020057555 @default.
• W2910786482 cites W2020330274 @default.
• W2910786482 cites W2023359102 @default.
• W2910786482 cites W2025771184 @default.
• W2910786482 cites W2026789880 @default.
• W2910786482 cites W2029374202 @default.
• W2910786482 cites W2031043669 @default.
• W2910786482 cites W2033950520 @default.
• W2910786482 cites W2041036779 @default.
• W2910786482 cites W2044550483 @default.
• W2910786482 cites W2047752768 @default.
• W2910786482 cites W2048517791 @default.
• W2910786482 cites W2056128904 @default.
• W2910786482 cites W2056615937 @default.
• W2910786482 cites W2059167716 @default.
• W2910786482 cites W2066209597 @default.
• W2910786482 cites W2071525767 @default.
• W2910786482 cites W2073824449 @default.
• W2910786482 cites W2079656901 @default.
• W2910786482 cites W2081097674 @default.
• W2910786482 cites W2082316399 @default.
• W2910786482 cites W2085683856 @default.
• W2910786482 cites W2099209753 @default.
• W2910786482 cites W2099903899 @default.
• W2910786482 cites W2099925220 @default.
• W2910786482 cites W2100177366 @default.
• W2910786482 cites W2101530434 @default.
• W2910786482 cites W2102629944 @default.
• W2910786482 cites W2105028012 @default.
• W2910786482 cites W2106631643 @default.
• W2910786482 cites W2108312821 @default.
• W2910786482 cites W2110260627 @default.
• W2910786482 cites W2112890360 @default.
• W2910786482 cites W2112910900 @default.
• W2910786482 cites W2115815903 @default.
• W2910786482 cites W2116091360 @default.
• W2910786482 cites W2119871660 @default.
• W2910786482 cites W2126879410 @default.
• W2910786482 cites W2127060683 @default.
• W2910786482 cites W2132522708 @default.
• W2910786482 cites W2133425351 @default.
• W2910786482 cites W2133552928 @default.
• W2910786482 cites W2133875949 @default.
• W2910786482 cites W2136305645 @default.
• W2910786482 cites W2137215017 @default.
• W2910786482 cites W2137279412 @default.
• W2910786482 cites W2137483855 @default.
• W2910786482 cites W2137846679 @default.
• W2910786482 cites W2141603177 @default.
• W2910786482 cites W2142783692 @default.
• W2910786482 cites W2143862901 @default.
• W2910786482 cites W2144141558 @default.
• W2910786482 cites W2145123043 @default.
• W2910786482 cites W2145982040 @default.
• W2910786482 cites W2146120613 @default.
• W2910786482 cites W2148633540 @default.
• W2910786482 cites W2150977134 @default.
• W2910786482 cites W2152190430 @default.

• next