FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2911040396> ?p ?o ?g. }

• W2911040396 endingPage "e0007088" @default.
• W2911040396 startingPage "e0007088" @default.
• W2911040396 abstract "Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote. Amastigote is the replication stage in host mammalian cells, hence this stage of parasite has clinical significance in drug development research. Presence of extracellular amastigotes (EA) and their infection capability have been known for some decades. Here, we demonstrate that EA can be utilized as an axenic culture to aid in stage-specific study of T. cruzi. Amastigote-like property of axenic amastigote can be sustained in LIT medium at 37°C at least for 1 week, judging from their morphology, amastigote-specific UTR-regulated GFP expression, and stage-specific expression of selected endogenous genes. Inhibitory effect of benznidazole and nifurtimox on axenic amastigotes was comparable to that on intracellular amastigotes. Exogenous nucleic acids can be transfected into EA via conventional electroporation, and selective marker could be utilized for enrichment of transfectants. We also demonstrate that CRISPR/Cas9-mediated gene knockout can be performed in EA. Essentiality of the target gene can be evaluated by the growth capability of the knockout EA, either by continuation of axenic culturing or by host infection and following replication as intracellular amastigotes. By taking advantage of the accessibility and sturdiness of EA, we can potentially expand our experimental freedom in studying amastigote stage of T. cruzi." @default.
• W2911040396 created "2019-01-25" @default.
• W2911040396 creator A5069755098 @default.
• W2911040396 creator A5086909167 @default.
• W2911040396 creator A5088578550 @default.
• W2911040396 creator A5089840214 @default.
• W2911040396 date "2019-01-14" @default.
• W2911040396 modified "2023-09-27" @default.
• W2911040396 title "Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi" @default.
• W2911040396 cites W1128029328 @default.
• W2911040396 cites W1569560012 @default.
• W2911040396 cites W1592463599 @default.
• W2911040396 cites W164772869 @default.
• W2911040396 cites W1943700220 @default.
• W2911040396 cites W1964051867 @default.
• W2911040396 cites W1983471417 @default.
• W2911040396 cites W1984702947 @default.
• W2911040396 cites W1988762847 @default.
• W2911040396 cites W1990497537 @default.
• W2911040396 cites W1992760440 @default.
• W2911040396 cites W1995816408 @default.
• W2911040396 cites W1997427580 @default.
• W2911040396 cites W2019461837 @default.
• W2911040396 cites W2021231316 @default.
• W2911040396 cites W2027217743 @default.
• W2911040396 cites W2027713759 @default.
• W2911040396 cites W2030452820 @default.
• W2911040396 cites W2036910636 @default.
• W2911040396 cites W2036992538 @default.
• W2911040396 cites W2041757695 @default.
• W2911040396 cites W2062231382 @default.
• W2911040396 cites W2064309587 @default.
• W2911040396 cites W2064879562 @default.
• W2911040396 cites W2071459755 @default.
• W2911040396 cites W2072631488 @default.
• W2911040396 cites W2073173819 @default.
• W2911040396 cites W2073512884 @default.
• W2911040396 cites W2076073407 @default.
• W2911040396 cites W2084326885 @default.
• W2911040396 cites W2087142903 @default.
• W2911040396 cites W2087503596 @default.
• W2911040396 cites W2092321887 @default.
• W2911040396 cites W2097796255 @default.
• W2911040396 cites W2098429260 @default.
• W2911040396 cites W2104970998 @default.
• W2911040396 cites W2119318585 @default.
• W2911040396 cites W2121746118 @default.
• W2911040396 cites W2124769246 @default.
• W2911040396 cites W2125977755 @default.
• W2911040396 cites W2127055793 @default.
• W2911040396 cites W2128450160 @default.
• W2911040396 cites W2150179875 @default.
• W2911040396 cites W2158889972 @default.
• W2911040396 cites W2194509219 @default.
• W2911040396 cites W2333694147 @default.
• W2911040396 cites W2340759119 @default.
• W2911040396 cites W2395646452 @default.
• W2911040396 cites W2403381246 @default.
• W2911040396 cites W2443441049 @default.
• W2911040396 cites W2542183658 @default.
• W2911040396 cites W2580394284 @default.
• W2911040396 cites W2614044637 @default.
• W2911040396 cites W2618421521 @default.
• W2911040396 cites W2769108218 @default.
• W2911040396 cites W2771049087 @default.
• W2911040396 cites W2775883868 @default.
• W2911040396 doi "https://doi.org/10.1371/journal.pntd.0007088" @default.
• W2911040396 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6347291" @default.
• W2911040396 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30640901" @default.
• W2911040396 hasPublicationYear "2019" @default.
• W2911040396 type Work @default.
• W2911040396 sameAs 2911040396 @default.
• W2911040396 citedByCount "13" @default.
• W2911040396 countsByYear W29110403962019 @default.
• W2911040396 countsByYear W29110403962020 @default.
• W2911040396 countsByYear W29110403962021 @default.
• W2911040396 countsByYear W29110403962022 @default.
• W2911040396 countsByYear W29110403962023 @default.
• W2911040396 crossrefType "journal-article" @default.
• W2911040396 hasAuthorship W2911040396A5069755098 @default.
• W2911040396 hasAuthorship W2911040396A5086909167 @default.
• W2911040396 hasAuthorship W2911040396A5088578550 @default.
• W2911040396 hasAuthorship W2911040396A5089840214 @default.
• W2911040396 hasBestOaLocation W29110403961 @default.
• W2911040396 hasConcept C104317684 @default.
• W2911040396 hasConcept C136764020 @default.
• W2911040396 hasConcept C2777625466 @default.
• W2911040396 hasConcept C2778265057 @default.
• W2911040396 hasConcept C2781092759 @default.
• W2911040396 hasConcept C41008148 @default.
• W2911040396 hasConcept C523546767 @default.
• W2911040396 hasConcept C54355233 @default.
• W2911040396 hasConcept C71928629 @default.
• W2911040396 hasConcept C77957584 @default.
• W2911040396 hasConcept C86803240 @default.
• W2911040396 hasConcept C95444343 @default.
• W2911040396 hasConcept C98319499 @default.
• W2911040396 hasConceptScore W2911040396C104317684 @default.

• next