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• W2911570563 abstract "Heat stress promotes intramyocyte calcium concentration ([Ca 2+ ] i ) accumulation via transient receptor potential vanilloid 1 (TRPV1) channels. We tested the hypothesis that muscle contractile activity concomitant with heat stress would accelerate the increase in [Ca 2+ ] i via TRPV1, further impairing [Ca 2+ ] i homeostasis. Spinotrapezius muscles of adult Wistar rats were exteriorized in vivo and loaded with the fluorescent Ca 2+ probe fura 2-AM. Heat stress (muscle surface temperature 40°C) was used as TRPV1 activator. An isometric contraction (100 Hz, 5–10 V, 30 s) was induced electrically concomitant with heat stress. [Ca 2+ ] i was determined for 20 min using in vivo fluorescence microscopy, and the phosphorylation response of TRPV1 was determined by Western blotting. Heat stress induced a significant [Ca 2+ ] i increase of 18.5 ± 8.1% at 20 min and TRPV1 phosphorylation (+231%), which was inhibited by addition of the TRPV1 inhibitor (capsazepine). However, contrary to expectations, the heat stress and isometric contraction condition almost completely inhibited TRPV1 phosphorylation and the consequent [Ca 2+ ] i elevation (<2.8% accumulation during heat stress, P > 0.05). In conclusion, this in vivo physiological model demonstrated that isometric muscle contraction(s) can suppress the phosphorylation response of TRPV1 and maintain [Ca 2+ ] i homeostasis during heat stress. NEW & NOTEWORTHY This investigation is the first document the dynamics of intramyocyte calcium concentration ([Ca 2+ ] i ) increase in the myoplasm of skeletal muscle fibers in response to heat stress where the muscle blood flow is preserved. Heat stress at 40°C drives a myoplasmic [Ca 2+ ] i accumulation in concert with transient receptor potential vanilloid 1 (TRPV1) phosphorylation. However, muscle contraction caused TRPV1 channel deactivation by dephosphorylation of TRPV1. TRPV1 inactivation via isometric contraction(s) permits maintenance of [Ca 2+ ] i homeostasis even under high imposed muscle temperature." @default.
• W2911570563 created "2019-02-21" @default.
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• W2911570563 date "2019-03-01" @default.
• W2911570563 modified "2023-10-16" @default.
• W2911570563 title "Accumulation of intramyocyte TRPV1-mediated calcium during heat stress is inhibited by concomitant muscle contractions" @default.
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• W2911570563 doi "https://doi.org/10.1152/japplphysiol.00668.2018" @default.
• W2911570563 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30676872" @default.
• W2911570563 hasPublicationYear "2019" @default.
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